Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for improving the endosperm vigor of Nepal pauliata seeds after cryopreservation

A technology for cryopreservation and seed embryos, which is applied in the field of plant germplasm resource preservation and can solve problems such as no research reports.

Active Publication Date: 2021-04-09
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research report on the storage of wild papaya seeds in Nepal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for improving the endosperm vigor of Nepal pauliata seeds after cryopreservation
  • A method for improving the endosperm vigor of Nepal pauliata seeds after cryopreservation
  • A method for improving the endosperm vigor of Nepal pauliata seeds after cryopreservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Materials and methods:

[0020] 1.1 Research materials:

[0021] In October 2014, mature fresh Mallotus nepalensis seeds were collected in Xintian Village, Fuxi Township, Huizhou District, Huangshan, Anhui. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0022] 1.2 Research methods:

[0023] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in step 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On September 14, 2015, the airtight container with the seeds was placed in a refrigerator at -18°C to...

Embodiment 2

[0037] 1. Materials and methods:

[0038] 1.1 Research materials:

[0039] On September 4, 2012, mature fresh Mallotus nepalensis seeds were collected in Wenshan County, Wenshan Prefecture, Yunnan Province. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0040] 1.2 Research methods:

[0041] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. On August 6, 2013, the airtight container with the seeds was put into -18°C to -20°C for refrigeration. After refri...

Embodiment 3

[0053] 1. Materials and methods:

[0054] 1.1 Research materials:

[0055] On October 08, 2009, fresh mature Mallotus nepalensis seeds were collected in Yuanjiujia Township, Pu'er Township, Yunnan Province. After collection, they were transported back to the Southwest Wild Organism Germplasm Bank Seed Preservation Center of Kunming Institute of Botany.

[0056] 1.2 Research methods:

[0057] 1.2.1 Drying and refrigerating and returning to temperature after refrigerating: put the seeds collected in 1.1 in a drying room at 15°C and 15% relative humidity to dry. Regularly use the Hygrolab C1unit relative humidity measuring instrument produced by Rotronic Ltd. combined with the Rotronic HC 2-AW probe produced by the same company to measure the relative humidity of the seeds, and put them into a closed container after the equilibrium relative humidity reaches 15%. In September 2011, the airtight container containing the seeds was placed in a refrigerator at -18°C to -20°C. Remo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for improving the vigor of endosperm of Nepal pauliata seeds after low-temperature storage, comprising: taking out the dry seeds stored at low temperature from -18°C to -20°C together with the airtight container for storing the seeds; The seeds were placed in an incubator at a temperature of 45°C for 24 hours, and the seeds were taken out after heat treatment for 6 hours. After the seeds returned to room temperature, they were placed on 1% agar medium, and placed in a 20°C culture room for 24 hours; the treated seeds were cut through the seed coat and endosperm, soaked in 1% triphenyltetrazolium chloride (TTC ) solution, placed in the dark at 30°C for 48 hours; the soaked seeds were taken out and cut to observe the endosperm staining, and the endosperm with vitality showed a uniform red color. The method significantly improves the endosperm vigor of the Nepal pauliata seeds stored at low temperature for different periods of time in different places of production. Solved the problem that the vigor of endosperm decreased after cryopreservation of wild pauliata seeds in Nepal.

Description

technical field [0001] The invention belongs to the technical field of preservation of plant germplasm resources, and in particular relates to a method for improving the endosperm vigor of jacaranda seeds preserved at low temperature (-18°C to -20°C). Background technique [0002] Mallotus nepalensis Müll.Arg. is a deciduous shrub or small tree species of the genus Mallotus Lour. in the family Euphorbiaceae. Distributed in the hillsides or valleys at an altitude of 1,700 to 2,500 meters in Yunnan, Tibet, and mainly distributed in Bhutan, Northeast India, Myanmar, and Nepal in foreign countries. There are few domestic research reports. Foreign reports have reported that its extract has a mild inhibitory effect on the central nervous system. There is no research report on the storage of wild papaya seeds in Nepal. [0003] Drying normal seeds and storing them at low temperature (-18°C to -20°C) can greatly prolong their lifespan, which has reached an international consensus, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 杨娟胡枭剑杜燕刘成何华杰杨娅娟李慧杨湘云
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com