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Hydrogel with micro-flow passage, as well as preparation method and application thereof

A technology of microfluidic channels and hydrogels, which is applied in biochemical equipment and methods, microbial measurement/inspection, medical science, etc., can solve problems such as high cost, cell damage, and complicated preparation methods, and achieve good mechanical strength, High controllability, support adhesion effect

Inactive Publication Date: 2013-10-23
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Therefore, the object of the present invention is to provide a hydrogel with microfluidic channels based on protein fiber generation, which has the disadvantages of complex preparation methods, high cost, uncontrollable structure of microfluidic channels, and damage to cells. The hydrogel of the channel and its preparation method and application, which are generated based on protein fibers, and the structure of the microfluidic channel is highly controllable, can be infused, has good biocompatibility, and has no harm to cells

Method used

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  • Hydrogel with micro-flow passage, as well as preparation method and application thereof
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  • Hydrogel with micro-flow passage, as well as preparation method and application thereof

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Effect test

Embodiment approach 1

[0054] Such as Figure 1-2 and Figure 5-7 As shown, the hydrogel 1 with a microfluidic channel according to the present invention includes a hydrogel base layer 7 and a hydrogel structure layer 6 bonded thereto, and the hydrogel structure layer 6 and the hydrogel base A microfluidic groove is provided on the bonded surface of the bottom layer 6, and the microfluidic groove and the hydrogel base layer 7 jointly form a microfluidic channel 2 for fluid circulation, and the hydrogel structural layer 6 or water On the other surface of the gel base layer 7 away from the bonding, perforations are respectively provided at positions corresponding to the two ports of the microfluidic channel 2 to form the channel inlet 4 and the channel outlet 5 of the microfluidic channel, so The hydrogel base layer 7 and the hydrogel structure layer 6 are all made of hydrogel containing sodium alginate and fibrous protein, and the hydrogel also contains alkaline earth metal salts, and the hydrogel b...

Embodiment approach 2

[0056] Such as Figure 3-4 As shown, the hydrogel with microfluidic channels described in the present invention has the same structure as the hydrogel with microfluidic channels in Embodiment 1, except that the shape of the microfluidic channels 2 is different.

[0057] Test materials used in the following examples: human umbilical vein endothelial cells (HUVEC cells) were purchased from the cell bank of Peking Union Medical College Hospital; MDCK kidney epithelial cells were purchased from the cell bank of Peking Union Medical College Hospital.

Embodiment 1

[0059] The first step: according to the shape of the required microfluidic groove, the specific size of the first mold with a protrusion compatible with the microfluidic groove is designed by CAD software, and the specific size of the second mold, said The width of the microfluidic groove is 200 μm, and the height is 200 μm, and the first and second molds of silicon rubber are made of silicon rubber materials by methods including photolithography and overmolding (see Microfluidic Chip Laboratory, Science Press, 2006); then on ice, mixed with sodium alginate (3% w / w) and type I collagen (3 mg / mL), and 10% DMEM 10X, 10% PBS 10X, 5 μL 1M NaOH and 100 μL deionized A hydrogel prepolymer in water is poured over the silicone rubber first mold.

[0060] The second step: increasing the temperature (to 37°C) and adding calcium chloride aqueous solution (60mM) to solidify type I collagen and sodium alginate hydrogel successively to form a hydrogel structure layer with microfluidic groove...

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Abstract

The invention provides hydrogel with a micro-flow passage, as well as a preparation method and an application thereof. The hydrogel comprises a hydrogel base layer, and a hydrogel structural layer bonded with the hydrogel base layer. The surface which is bonded with the hydrogel base layer, of the hydrogel structural layer is provided with a groove structure; the groove structure, together with the hydrogel base layer, forms a micro-flow passage for allowing a fluid to flow in; the other surface of the hydrogel structural layer or the hydrogel base layer which deviates from bonding is respectively provided with through holes corresponding to two ports of the micro-flow passage to form a passage inlet and a passage outlet of the micro-flow passage; the hydrogel base layer and the hydrogel structural layer are both prepared from hydrogel containing sodium alginate and fibrous protein; and the hydrogel base layer and the hydrogel structural layer are bonded by protein fibers formed by the fibrous protein. The hydrogel with the micro-flow passage can be applied to a vascular system in a simulated living body or applied to preparation of kits for biological detection.

Description

technical field [0001] The invention relates to a hydrogel with a microfluidic channel, a preparation method and application thereof, and belongs to the technical field of tissue engineering. Background technique [0002] Hydrogels with microchannel structures can play a very important role in the preparation of functional materials, as well as in the simulation and reconstruction of vascularized tissues in vitro. By fabricating suitable microchannel structures in hydrogels, it is possible to simulate the vasculature in organisms (such as blood vessels, lymphatic vessels, renal tubules, and plant ducts). This is also the basis for mimicking tissue function in vivo in vitro. In addition, microchannels in hydrogels can also be used to control the local physical and chemical properties of hydrogels. However, hydrogel is a kind of hydrophilic polymer material, and its unique physical and chemical properties determine that it is extremely difficult to construct the required mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/52A61L27/50A61L29/12A61L29/14C12Q1/02G01N33/50
Inventor 蒋兴宇慕轩肖乐张伟郑文富
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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