Coxsackie virus A16-type virus strain and applications thereof

Abstract

The invention provides a coxsackie virus A16-type virus strain. The collection number of the coxsackie virus A16-type virus strain is CGMCC No.5372, wherein CGMCC refers to China General Microbiological Culture Collection Center. The virus is a 20-face stereoscopic symmetrical sphere under observation through an electron microscope, and the diameter of the virus is 23-30nm. VP1 conserved region sequence analysis and mass spectrum analysis are respectively performed on the virus strain, and a result shows a CA16 virus. The CA16 virus can be efficiently proliferated in Vero cells (African green monkey kidney cells), and the virus titer can reach 6.61g CCID50 / ml. Moreover, the virus strain has no external pollution, better immunogenicity and a good effect.

Description

technical field

[0001] The invention relates to the fields of virology and molecular biology technology, in particular to a new Coxsackievirus A16 virus strain and application thereof. Background technique

[0002] Hand foot mouth disease (HFMD) is an infectious disease caused by enterovirus, which mostly occurs in children under 5 years old, and can cause herpes on the hands, feet, mouth and other parts, and a small number of children can cause pulmonary edema, asymptomatic Bacterial meningoencephalitis and other complications. In some severely ill children, if the disease develops rapidly, it will lead to death. There are more than 20 types (types) of enteroviruses that cause hand, foot and mouth disease. Coxsackievirus types 16, 4, 5, 9, and 10 in group A, types 2 and 5 in group B, and enterovirus type 71 are all It is the most common pathogen of hand, foot and mouth disease. It is mainly transmitted through fecal-oral, respiratory and close contact, and can be transmi...

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