Porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and preparation method thereof

Abstract

The invention relates to a porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and a preparation method of the porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine. The porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine is prepared by performing virus amplification on a swine testicular cell line (ST cells) or an African green monkey kidney cell line (Vero cells) by using a self-attenuated and preserved transmissible gastroenteritis virus SD / L strain and a self-attenuated and preserved porcine epidemic diarrhea virus LW / L strain, and carrying out the steps of harvesting, uniformly mixing, freeze-drying and the like. The porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine can effectively prevent two diseases namely swine transmissible gastroenteritis and epidemic diarrhea.

Description

technical field [0001] The invention relates to a porcine transmissible gastroenteritis and epidemic diarrhea dual live vaccine, which belongs to the field of veterinary biological products. Background technique [0002] Porcine transmissible gastroenteritis is a highly contagious, fatal infectious disease of pigs caused by Transmissible gastroenteritis virus (TGEV), which causes vomiting, severe diarrhea and dehydration in piglets under 2 weeks of age as the main feature. Among them, the mortality rate of piglets within 2 weeks of age can reach 100%, and the mortality rate of pigs over 5 weeks of age is relatively low. Larger or adult pigs hardly die, but it can cause fat loss, reduce feed remuneration, increase medicine and manpower expenses, etc. , causing serious losses. The disease has been widely prevalent in the United States since 1933. The pathogen of the disease was determined by Doyle et al. in 1946. After that, many countries and regions in the world (Japan, Br...

AI Technical Summary

Problems solved by technology

For domestic porcine transmissible gastroenteritis and porcine epidemic diarrhea dual live vaccines, the cell matrix for virus culture is mostly primary cells, and primary cells are composed of various types of cells. The cell types are more complex and the characteristics of cells are different. , there are differences among individual animals, and there are disadvantages such as difficulty in ensuring the consistency of cell quality, high risk of contamination by exogenous pathogenic microorganisms, and high production costs, which bring difficulties to the improvement of vaccine yield and efficacy, and are difficult to meet large-scale production

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