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Application of vitamin B12 in preparation of medicine for resisting novel coronavirus SARS-CoV-2

A coronavirus, B12 technology, applied in the field of medicine, can solve problems such as the application of vitamin B12 that has not yet been seen

Pending Publication Date: 2022-02-22
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of medical treatment, vitamin B12 is mainly used to prevent and treat anemia caused by various reasons, and to supplement the increased demand for vitamin B12 caused by wasting diseases, hyperthyroidism, pregnancy, breast-feeding, etc. In addition, it is also used for neuritis, Non-specific treatment of neuralgia, etc.; the application of vitamin B12 in inhibiting the infection and replication of coronaviruses, especially novel coronaviruses, has not been seen

Method used

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  • Application of vitamin B12 in preparation of medicine for resisting novel coronavirus SARS-CoV-2
  • Application of vitamin B12 in preparation of medicine for resisting novel coronavirus SARS-CoV-2
  • Application of vitamin B12 in preparation of medicine for resisting novel coronavirus SARS-CoV-2

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Real-time quantitative PCR (qRT-PCR) method was used to detect the virus copy number of Vero-E6 cell supernatant to verify the inhibitory effect of vitamin B12 on the infection of SARS-CoV-2:

[0029] 1. Inoculate Vero-E6 cell suspension (4×10 4 per well), the culture plate was placed in the incubator for pre-cultivation for 12 hours, and the cells adhered to the wall for growth;

[0030] 2. Add the corresponding concentration of vitamin B12 to treat for 1 hour in advance, add DMSO to the solvent control, and add no drugs to the negative control;

[0031] 3. After that, add 200PFU SARS-CoV-2 virus (GenBank: MT121215.1) to each well except the negative control. After 12 hours of infection, wash twice with PBS, add new medium containing vitamin B12, and incubate at 37°C After 48 hours, the cytopathic changes were observed under the microscope.

[0032] 4. After 48 hours, collect the cell supernatant for RNA extraction.

[0033] RNA extraction

[0034] 1. Add 100 μL of...

Embodiment 2

[0045] To detect the toxic effect of vitamin B12 on Vero-E6 cells:

[0046] 1. Inoculate Vero-E6 cell suspension (1×10 4 per well), the culture plate was placed in the incubator for 12 hours;

[0047] 2. Add different concentrations of the drug to be tested to the culture well, and the control group is the solvent DMSO;

[0048] 3. After 48 hours, detect the effect of the drug on cell proliferation. Use colleagues CCK-8 endpoint method kit (ck04). Discard the excess medium in the well, and add 100 μL of serum-free medium containing 10% CCK8 solution to each well;

[0049] 4. Place the culture plate in the incubator and incubate for 1-4 hours;

[0050] 5. Then use a microplate reader to measure the absorbance at 450 nm.

[0051] Experimental results such as image 3 as shown,

[0052] Vitamin B12 has no cytotoxicity to Vero-E6 cells when the action concentration is below 320μM; its half cytotoxic concentration (CC50) is higher than 640μM.

[0053] In summary, the experi...

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Abstract

The invention belongs to the technical field of medicines, and relates to a novel application of vitamin B12 in pharmacy, in particular to an application of vitamin B12 in preparation of a medicine for resisting novel coronavirus. Experiments prove that the vitamin B12 can inhibit infection of the novel coronavirus on in-vitro cell culture Vero-E6 (African green monkey kidney cells), new application of the vitamin B12 in treatment of the novel coronavirus caused by the novel coronavirus is developed, and a new medicine is provided for treatment and control of the novel coronavirus.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to the new application of vitamin B12 in pharmacy, in particular to the application of vitamin B12 in the preparation of anti-new coronavirus SARS-CoV-2 drugs. Background technique [0002] The prior art discloses that novel coronavirus disease (Corona Virus Disease 2019, COVID-19), also known as novel coronavirus pneumonia, is a severe acute respiratory infectious disease. The disease is highly infectious and has a high fatality rate. The pathogen that causes the new coronavirus disease is a new type of coronavirus, which was named 2019-nCoV by the World Health Organization on January 2, 2020, and named SARS-CoV-2 by the International Virus Taxonomy Committee on February 11, 2020. SARS-CoV-2 and the known severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) belong to the betacoronavirus genus of the Coronaviridae family...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/714A61P31/14A61P11/00
CPCA61K31/714A61P31/14A61P11/00
Inventor 谢幼华吴旸谷陈建郭慧敏朱园飞邓强刘晶
Owner FUDAN UNIV
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