229 results about "In vitro cell culture" patented technology

This invention relates to polymeric nanoparticles useful for drug delivery with target molecules bonded to the surface of the particles and having sizes of up to 1000 nm, preferably 1 nm to 400 nm, more preferably 1 nm to 200 nm, that are dispersed homogeneously in aqueous solution. The target drug/target substance is covalently bonded to the novel polymeric nanoparticles to secure them from outer intervention in vivo or cell culture in vitro until they are exposed at the target site within the cell. This invention also relates to microemulsion polymerization techniques useful for preparing the novel nanoparticles.

Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.

Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.

The invention relates to a chitosan modified alginate hydrogel three-dimensional porous bracket with specific in-vitro degradability and a preparation method thereof. The method comprises the following steps: dissolving sodium alga acid serving as a raw material in phosphate buffer solution; performing amidation reaction of a carboxyl group in the sodium alga acid and an amino group in a cross-linking agent cystamine or dimethyl cystinate under the activation of water-soluble carbodiimide to form a chemically crosslinked hydrogel; performing freeze drying on the hydrogel to obtain a porous bracket material of the hydrogel; and performing surface modification on the porous bracket by using chitosan. In solution of a reducing agent such as cysteine with an appropriate concentration, a disulfide bond in a hydrogel cross-bridge is degraded through a disulfide bond-sulfydryl conversion reaction, so the porous bracket is decomposed and dissolved and disappears. Therefore, the porous bracket can be used as an in-vitro cell culture template material. The hydrogel porous bracket researched by the invention has the characteristics of simple preparation, rich raw material source, low cost and availability. Various physiochemical performances, mechanical strength, degradation rate and surface properties of the bracket material are controllable within a large range.

Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.

The invention provides an application of a type of berbamine derivatives and salts thereof in the preparation of drugs for the treatment of tumors, which is mainly applied in the preparation of the drugs for the prevention and treatment of nuclear transcription factor NF-kBp65 activity-related diseases and BCR/ABL transcription activity-related diseases. The drugs are combined and prepared by the compounds of the invention and one or more pharmaceutically acceptable excipients. The preparation forms comprise solid preparations, semi-solid preparations or liquid preparations. The type of berbamine derivatives and the salts thereof provided by the invention have broader and stronger anti-leukemia and anti-solid-tumor activity, the tumors proved to be sensitive are leukemia, multiple myeloma, liver cancer, osteosarcoma and breast cancer; the toxicity and the side effects are lighter. An in vitro cell culture system and animal experiments confirm that the berbamine derivatives and the salts thereof have no significant toxicity or side effects to the growth of normal human hematopoietic cells and experimental animals under the anti-tumor dosage, which are superior to the commonly used chemotherapy drugs.

Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.

The invention discloses a nanometer colloid particle-assembled high-strength self-repairing injectable composite colloid gel material and a preparation method and application thereof. According to the preparation method, two-phase colloid particles with opposite electric charges are uniformly blended in an alkaline or acidic environment, an acidifying agent or alkalizing agent is added to induce the pH of the solution to recover the neutrality, electrostatic self-assembly among the two-phase colloid particles is initiated, and a uniformly-dispersed composite gel network is formed, wherein the gel is high in mechanical strength and wide in regulation and control range, the elasticity modulus of the gel can be regulated and controlled within the range of 10 Pa-100 kPa, and the self-repairing efficiency of the gel is higher than or equal to 100%. The silicon dioxide-gelatin composite colloid gel which has both the high mechanical strength and the self-repairing performance is finally and successfully prepared, and it is verified that the composite gel still can quickly recover the mechanical strength after being broken by shear force many times; in addition, the composite gel has the excellent injectable property and plasticity and can be used for an in-vitro cell culture matrix material by serving as an injectable biological material.

The invention belongs to the technical field of cell culture in vitro, and particularly relates to a preparation method of high-purity, high-multiplication capacity and high-cytotoxin activity CIK (cytokine induced kill) cell. The method comprises the following steps: collecting and separating peripheral blood mononuclear cell of a patient, eliminating CD4+CD25+Treg cell by means of Mini MACS (magnetic active cell sorting) method, and sorting to obtain CD3+, CD4+ and CD8+T cells; and putting the obtained cells into culture solution containing phytohemagglutinin (PHA), so that the PHA concentration in the suspension liquid is 100ng/ml, hatching for 24h under the culture condition of 5% CO2 at 37 DEG C, transferring the hatched suspension liquid into a cell culture bottle coated by CD3 monoclonal antibody (1mug/ml), adding IFN (interferon)-gamma (1000U/ml), adding IL (interleukin)-2(500U/ml) and IL (interleukin)-21(1000U/ml) after 48h, compensating sodium selenite-containing (0.005mg/L)cell culture after four days, and continuously culturing for 7-14 days to obtain the high-purity, high-multiplication capacity and high-cytotoxin activity CIK (cytokine induced kill) cell. The quantity, the activity and the purity of the CIK cell which is prepared by the method and amplified in vitro are improved, so that the antineoplastic function of the CIK is enhanced.

The invention provides in vitro cell culture compositions consisting of neurospheres and culture medium, wherein the neurospheres consist of undifferentiated cells that are nestin⁺, glial fibrillary acid protein (GFAP)⁻, neurofilament (NF)⁻, and myelin basic protein (MBP)⁻ and are not nestin⁻.

The invention relates to the technical field of medicaments. Dimethyldiguanide belongs to a biguanides oral hypoglycemic medicament and is widely applied to pharmaceutical therapy of type 2 diabetes. The invention aims at providing a novel application of the dimethyldiguanide and particularly an application of the dimethyldiguanide in preparation of medicaments for preventing or treating the hepatocellular carcinoma. According to the application disclosed by the invention, the restriction effect of the dimethyldiguanide on the hepatocellular carcinoma is found by research from two aspects, namely, cell culture in vitro and animal experiment in vivo. Experiments find that the dimethyldiguanide has the capabilities of restricting proliferation and clone formation of a hepatocellular carcinoma cell line and causing the cell cycle arrest and apoptosis increase of the hepatocellular carcinoma cell line, and also has the capability of enhancing the hepatocellular carcinoma resisting effect of chemotherapeutics when combined with the chemotherapeutics, such as cis-platinum, doxorubicin and the like. The invention provides the novel application of the dimethyldiguanide and also provides a novel concept for prevention and treatment of the hepatocellular carcinoma.

The invention discloses an in vitro cell culture device. The in vitro cell culture device comprises a bioreactor, a double nutrient solution irrigating system, a loading device and a monitoring system. The invention also discloses a method for culturing the cell by using the in vitro cell culture device. The method comprises the following steps: biologically simulating a physiological status, establishing the double nutrient solution irrigating system, and supplying different nutrients to different tissue blocks; allocating two pipe systems for each double culture cup, taking the culture tissue as a spacer layer of two culture solutions, culturing by simultaneously using two culture solutions, automatically irrigating the culture solutions, timely and quantificationally changing the cell culture solution, regulating the irrigating speed of the culture solution and constantly providing various nutrients and stable pH value for the cell. A dynamic pressurized environment is adopted for the cell culture device and the cell culture method; a pressure is exerted on the culture tissue by exerting a controllable axial compressive deformation load; the pressure and the compressive deformation of each culture tissue can be monitored and analyzed in real time and the pressure can be adjusted.

The invention belongs to the technical field of in-vitro cell culture, and relates to a culture medium for culturing T lymphocyte. The culture medium comprises a basic culture medium and an additive for replacing human serum or cattle serum, and the additive comprises recombinant human albumin, recombinant human transferrin, recombinant human insulin, cholesterol, oleic acid, and linoleic acid. The provided culture medium has the following advantages: (1) the provided culture medium is safer, and the safety problem caused by human original and animal original components is avoided; (2) the proliferation multiple of T lymphocyte, which is cultured by the provided culture medium, is higher than that of T lymphocyte, which is cultured by a comparative culture medium.

The invention relates to the field of biological three-dimensional printed meniscus bracket, in particular to a biological three-dimensional printing preparation method of a chondrocyte loading anti-inflammatory meniscus bracket. The biological three-dimensional printing preparation method of the chondrocyte loading anti-inflammatory meniscus bracket can well simulate a three-dimensional macroscopic morphology, interior microcosmic collagen fibers and a porous structure of meniscus tissues, is beneficial to cell growth and exchange of nutrients and promotes regeneration of the meniscus tissues. For the biological three-dimensional printing preparation method of the chondrocyte loading anti-inflammatory meniscus bracket, by selecting polycaprolactone to print a frame structure of the meniscus bracket, enough mechanics is provided to joint support; meanwhile, by loading chondrocytes, operation of cell culture in vitro after bracket printing is avoided; and by loading a fullerene material, the bracket has the anti-inflammatory effect.

A 3D scaffold is prepared from the vegetative gliadin, the pore-forming agent chosen from edible salt, ammonium dicarbonate, ammonium acetate, etc. the polylactic acid or polyhydroxyacetic acid, and the biomedical material (collagen,chitosan, etc) through proportional mixing and natural shaping or die pressing. Its advantages are high degradability and bacterial resistance, low immunogenicity and proper mechanical strength.

The invention discloses a photon stimulated cell desorption method and a cell culture implement used by the same. The method comprises the following steps: preparing a photosensitive semiconductor structure layer on the cell contact surface of a cell culture vessel as the cell culture surface; then performing in-vitro cell culture on the cell culture surface of the cell culture vessel; and after completing the in-vitro cell culture, performing ultraviolet light or visible light irradiation treatment to desorb cells growing on the cell culture surface from the cell culture vessel. According to the method disclosed by the invention, injuries to the cells can be reduced to the greatest extent during cell desorption. The cell culture implement used in the method comprises the cell culture vessel, and the photosensitive semiconductor structure layer is prepared on the cell contact surface of the cell culture vessel as the cell culture surface. The implement can be realized by performing very small improvement on the basis of the prior art and has the advantages of low cost and easiness in popularization and application.

The invention discloses a three-dimensional micro-carrier cell adsorption culture method which comprises the following steps: 1) carrying out cell inoculation, namely mixing a cell suspension with a dried three-dimensional micro-carrier so as to obtain a micro-carrier mixed with the cell suspension; 2) carrying out cell adsorption, namely incubating the micro-carrier mixed with the cell suspensionobtained in the step 1) by using a rotational dynamic adsorption method, and enabling cells to be adhered to the three-dimensional micro-carrier; 3) carrying out cell culturing, namely culturing witha complete culture medium after the cells in the step 2) are adhered. By adopting the method, a large growth area is provided for cells, operation that the cells are inoculated with the micro-carrieris simplified, and the risk of contamination is avoided; the method is applicable to culture of large-scale bioreactors; due to the unique imbibitions property of the three-dimensional micro-carrier,a great number of cells can be fed into pores communicated with the micro-carrier, then a bionic three-dimensional growth mode can be well formed, and functions can be brought into play in the in-vitro cell culture process.

The invention provides polymeric microspheres and a preparation method therefore. The polymeric microspheres are obtained through physical crosslinking by mixing emulsified polymeric liquid drops and a solution containing a fixing agent. Compared to the prior art, by adopting physical crosslinking, in a preparation process, no crosslinking agents for chemical crosslinking with relatively great poison are not introduced, so that the purity of the obtained polymeric microspheres is relatively high; moreover, the polymeric microspheres have extracellular matrix simulating structures which are three-dimensional nano fiber network structures. Furthermore, the polymeric microspheres in an in vitro cell culture test show a good adhesive proliferation characteristic, and cartilage tissue blocks are constructed in vitro successfully after cartilage cells are compounded, so that the polymeric microspheres have important application value in the bio-medical field.