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Polymeric microspheres and preparation method therefor

A technology of polymers and microspheres, applied in the field of biomimetic materials, can solve the problems of difficult washing and removal, polymers containing cross-linking agents, and difficulty in obtaining microsphere materials with an extracellular matrix-like structure, etc., and achieve good adhesion. Proliferative properties, high purity effect

Active Publication Date: 2015-11-25
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology

[0005] However, it is difficult to obtain microsphere materials with a structure imitating extracellular matrix in the currently disclosed microsphere preparation methods, and the cross-linking process is chemical cross-linking, and the cross-linking agents used are all highly toxic chemical reagents, making cross-linking difficult. Linked polymers contain toxic crosslinkers that are difficult to remove by washing

Method used

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  • Polymeric microspheres and preparation method therefor
  • Polymeric microspheres and preparation method therefor
  • Polymeric microspheres and preparation method therefor

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preparation example Construction

[0026] The invention provides a method for preparing polymer microspheres, comprising:

[0027] The emulsified polymer droplets are mixed with a solution containing a fixative to obtain polymer microspheres through physical cross-linking.

[0028] According to the present invention, the present invention obtains polymer microspheres through physical crosslinking by mixing emulsified polymer droplets with a solution containing a fixative;

[0029] Wherein, the polymer in the emulsified polymer droplets is preferably one or more of chitosan, collagen, silk protein, gelatin and sodium alginate, more preferably chitosan or sodium alginate;

[0030] The solution containing the fixing agent is alcohol solution of sodium hydroxide, alcohol solution of potassium hydroxide, alcohol solution of calcium chloride, alcohol solution of calcium nitrate, acetone or methanol; the alcohol in the alcohol solution is methanol and ethanol One or both of them; when the solution containing the fixi...

Embodiment 1

[0040] Weigh 0.5g of chitosan and dissolve it in 50ml of 1% acetic acid aqueous solution to prepare a uniform chitosan aqueous solution; use a 10ml syringe to extract 5-8ml of the prepared chitosan solution and insert it into the inner tube of the tube-in-tube microfluidic device Utilize a 100ml syringe to extract 50-80ml cyclohexane (containing 3% Span-80) as the extrusion phase, and insert it into the outer tube of the tube casing microfluidic device; The diameter of the tube is 60 microns, the extrusion speed is 0.01ml / min, the outer tube is directly 300 microns, and the extrusion speed is 0.4ml / min), the NaOH ethanol solution of 0.3mol / L is prepared as a solution containing a fixative to collect the extruded liquid microspheres; obtaining cross-linked chitosan microspheres with a diameter of 100 micrometers; washing the cross-linked chitosan microspheres with ethanol and finally supercritical drying to obtain chitosan microspheres.

[0041] In the same way, by controlling ...

Embodiment 2

[0047] Weigh 0.5g of sodium alginate and dissolve it in 50ml of water to prepare a uniform sodium alginate aqueous solution; use a 10ml syringe to extract 5-8ml of the prepared sodium alginate solution, insert it into the inner tube of the tube-in-tube microfluidic device, and use a 100ml syringe Extract 50-80ml cyclohexane (containing 3% Span-80) as the extrusion phase, insert the outer tube of the tube casing microfluidic device; use a micro injection pump to control the extrusion speed of the two syringes respectively (the inner tube is directly 60 microns , the extrusion speed is 0.03ml / min, the outer tube is directly 300 microns, the extrusion speed is 0.6ml / min), and the CaCl of 0.01mol / L is prepared 2 The ethanol solution is used as a solution containing a fixative to collect the extruded liquid microspheres; obtain cross-linked sodium alginate microspheres; wash the cross-linked sodium alginate microspheres with ethanol and finally undergo supercritical drying to obtain...

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Abstract

The invention provides polymeric microspheres and a preparation method therefore. The polymeric microspheres are obtained through physical crosslinking by mixing emulsified polymeric liquid drops and a solution containing a fixing agent. Compared to the prior art, by adopting physical crosslinking, in a preparation process, no crosslinking agents for chemical crosslinking with relatively great poison are not introduced, so that the purity of the obtained polymeric microspheres is relatively high; moreover, the polymeric microspheres have extracellular matrix simulating structures which are three-dimensional nano fiber network structures. Furthermore, the polymeric microspheres in an in vitro cell culture test show a good adhesive proliferation characteristic, and cartilage tissue blocks are constructed in vitro successfully after cartilage cells are compounded, so that the polymeric microspheres have important application value in the bio-medical field.

Description

technical field [0001] The invention belongs to the field of bionic materials, in particular to a polymer microsphere and a preparation method thereof. Background technique [0002] Biological tissue provides a three-dimensional growth environment for cells through the network structure of extracellular matrix nanofibers and determines the fate of cells; tissue regenerative medicine is the use of tissue engineering scaffold materials combined with target cells for artificial tissue culture in vitro or directly grafting scaffold materials to defective tissues. In vivo tissue reconstruction; therefore, as a tissue engineering scaffold material, it is necessary to simulate the structural characteristics of the extracellular matrix to the greatest extent, so as to be suitable for cell adhesion and growth. [0003] At present, researchers have adopted an effective idea to build cell microcarrier scaffold materials as the carrier of cell adhesion growth, which can not only obtain ...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L5/08C08L89/00C08L5/04B01J13/14A61L27/20A61L27/24A61L27/22A61L27/50
Inventor 俞书宏高怀岭邹多宏周咏
Owner UNIV OF SCI & TECH OF CHINA
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