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Photon stimulated cell desorption method and cell culture implement used by same

A cell culture and in vitro cell culture technology, applied in the field of tissue engineering, can solve the problems of damaged cells and long time, and achieve the effects of small cell damage, high efficiency and easy realization

Inactive Publication Date: 2012-07-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it still takes a long time to use temperature as an external field, and the required low temperature process can damage the function of cells to a certain extent

Method used

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  • Photon stimulated cell desorption method and cell culture implement used by same
  • Photon stimulated cell desorption method and cell culture implement used by same
  • Photon stimulated cell desorption method and cell culture implement used by same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Titanium oxide micro-nano lattices were prepared on the cell contact surface of quartz glass cell culture vessels by phase-splitting self-assembly method, the process is as follows: according to the preparation of ethanol: deionized water: acetylacetone = 0.3: 1: 1 (in molar ratio) solution, and add tetra-n-butyl titanate by 0.02mol / L, and add polyvinylpyrrolidone (K-30) by 40g / L to form a precursor solution, which is added dropwise on the cell contact surface of the quartz glass cell culture vessel, Spin coating at a speed of 6000 rpm for 40 s, and then heat-treat at 500 °C for two hours to obtain titanium oxide micro-nano lattices as a cell culture surface. The density of nano-dots in the titanium oxide micro-nano lattice is 1.0×10 10 / cm 2 , The dot size is 200nm ~ 500nm.

[0030] Bone cells were cultured in vitro on the cell culture surface of the above-mentioned quartz glass cell culture vessel. After three days of culture, ultraviolet light with a wavelength of ...

Embodiment 2

[0036] A zinc oxide thin film is prepared on the cell contact surface of a silicate glass cell culture vessel by a chemical vapor deposition method as a cell culture surface, the grain size of the zinc oxide thin film is 2-10nm, and the thickness is 50nm.

[0037] Fibroblasts were cultured in vitro on the cell culture surface of the above-mentioned silicate glass cell culture vessel. After culturing for three days, ultraviolet light with a wavelength of 300 nanometers was incident from the bottom of the cell culture vessel and irradiated for 1 minute to make 70% of the cells from Surface detachment.

Embodiment 3

[0039] A titanium oxide thin film is prepared on the cell contact surface of the polylactic acid cell culture vessel by a physical vapor deposition method as the cell culture surface, the grain size of the titanium oxide thin film is 10-30nm, and the thickness is 1000nm.

[0040] Fibroblasts were cultured in vitro on the cell culture surface of the above-mentioned polylactic acid cell culture vessel. After three days of culture, ultraviolet light with a wavelength of 365 nanometers was incident from the bottom of the cell culture vessel and irradiated for 25 minutes to detach 74% of the cells from the surface. .

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Abstract

The invention discloses a photon stimulated cell desorption method and a cell culture implement used by the same. The method comprises the following steps: preparing a photosensitive semiconductor structure layer on the cell contact surface of a cell culture vessel as the cell culture surface; then performing in-vitro cell culture on the cell culture surface of the cell culture vessel; and after completing the in-vitro cell culture, performing ultraviolet light or visible light irradiation treatment to desorb cells growing on the cell culture surface from the cell culture vessel. According to the method disclosed by the invention, injuries to the cells can be reduced to the greatest extent during cell desorption. The cell culture implement used in the method comprises the cell culture vessel, and the photosensitive semiconductor structure layer is prepared on the cell contact surface of the cell culture vessel as the cell culture surface. The implement can be realized by performing very small improvement on the basis of the prior art and has the advantages of low cost and easiness in popularization and application.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a photoinduced cell detachment method and a cell culture device used therein. Background technique [0002] In vitro cell culture (in vitro cell culture) is an indispensable technology in the research of biomedical technology and biomaterials. How to reduce the adverse effects of external uncertain factors on cell behavior in in vitro cell culture, make it effectively attach, proliferate, differentiate, and separate from the medium when needed, so as to reflect the response to drugs or materials at the cell level, these Directly affect the research and development of new drugs and new medical materials. [0003] Many of the cells involved in in vitro cell culture are adherent cells. Cell culture is usually carried out in polystyrene cell culture equipment, and adherent cells are generally relatively firmly attached to the surface of the cell culture equipment throu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/077C12N5/071C12M3/00
CPCC12M25/00
Inventor 程逵翁文剑洪逸
Owner ZHEJIANG UNIV
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