14 results about "Cells isolation" patented technology

A system and method for early detection of thermal runaway and electrochemical degradation in battery cells and packs includes a sensor with at least two conductive electrodes separated by a sub-millimeter gap and biased by a controlled voltage. When thermal runaway byproducts enter the gap, current between the electrodes is altered to produce a measurable signal, conditioned by onboard or remote circuitry through amplification / filtering to generate a processed signal. This signal is transmitted to a battery control system to initiate protective actions (e.g., shutdown, cooling activation, or cell isolation). The sensor may be implemented on a printed circuit board or as discrete electrodes and may include a gas-permeable filter that admits vapors and fine aerosols while blocking liquids and debris. The disclosed system enables real-time, sub-second detection of early thermal runaway precursors before temperature rise or combustion occurs, enhancing safety and reliability of lithium-ion and other electrochemical energy storage systems.

A system and method for early detection of thermal runaway and electrochemical degradation in battery cells and packs includes a sensor with at least two conductive electrodes separated by a sub-millimeter gap and biased by a controlled voltage. When thermal runaway byproducts enter the gap, current between the electrodes is altered to produce a measurable signal, conditioned by onboard or remote circuitry through amplification / filtering to generate a processed signal. This signal is transmitted to a battery control system to initiate protective actions (e.g., shutdown, cooling activation, or cell isolation). The sensor may be implemented on a printed circuit board or as discrete electrodes and may include a gas-permeable filter that admits vapors and fine aerosols while blocking liquids and debris. The disclosed system enables real-time, sub-second detection of early thermal runaway precursors before temperature rise or combustion occurs, enhancing safety and reliability of lithium-ion and other electrochemical energy storage systems.

The present disclosure relates to novel Spodoptera frugiperda cell lines free of Sf9 rhabdovirus, methods for making and methods for using the cell lines. In one aspect, the method includes single cell isolation to obtain a cloned cell line and to identify deletion of Sf9 rhabdovirus. In one aspect, the disclosure provides novel cell lines and kits comprising the cell lines. In one aspect, the disclosure provides a method for producing a biological product using the cell line.

The present invention belongs to the technical field of biomedicine. Disclosed are stem-cell-derived apoptotic extracellular vesicles (ApoEVs) having an iron homeostasis regulatory effect and the use thereof in the preparation of a drug for treating iron deficiency anemia. ApoEVs are extracellular vesicles isolated from apoptotic cells derived from human periodontal ligament stem cells, and can effectively restore low intracellular iron levels induced by deferoxamine mesylate (DFOM), upregulate the expression of iron storage proteins and regulate the levels of iron export proteins, thereby improving cellular iron homeostasis. Moreover, the ApoEVs have good biocompatibility and safety, and provide a new strategy for the treatment of iron deficiency anemia.

The invention belongs to the technical field of biology, and particularly relates to a nucleus pulposus cell separation and culture method. According to the method, a step-by-step enzyme digestion method is adopted, NB6 collagenase is firstly used for primary digestion, and then compound enzyme liquid composed of trypsin and hyaluronidase is adopted for subsequent digestion; and culturing in combination with a culture vessel coated with a specific concentration of extracellular matrix component and a special culture medium. According to the method, on the premise of not depending on an immortalization technology, the primary nucleus pulposus cells with good activity can be efficiently obtained, stable amplification and long-term passage of the primary nucleus pulposus cells in vitro are realized, and specific marker expression and biological functions of the nucleus pulposus cells can be effectively maintained. The invention provides a stable cell source and technical platform for the development of cell therapy products and the research of spinal degenerative diseases.

A 3D NAND flash memory device and fabricating method thereof, comprises: First, a semiconductor substrate layer is provided, wherein cell isolation structures, channel structures and source lead-out spaces are formed on the semiconductor substrate layer, with the cell isolation structures including cell isolation layers and memory cell-occupied spaces. First dielectric layers and second dielectric layers are sequentially deposited, the first dielectric layer covering at least an exposed surface of the cell isolation structure. The second dielectric layers are then etched to remove all of the second dielectric layers on the side surface and at least part of the second dielectric layers on the second surface, and all of the second dielectric layers on the first surface is retained, with the remaining second dielectric layer forming at least one electron capture layer for memory cell structures. The memory cell structures and electrode structures for the flash memory device are finally fabricated.

According to the embodiments provided herein, a photovoltaic device may have one or more cells with in-cell isolation.

The invention provides a sheep embryo fibroblast line and application thereof, and belongs to the technical field of cell biology. The name of the sheep embryo fibroblast line is sheep embryo fibroblast OEF, the sheep embryo fibroblast line is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is January 26, 2022, and the preservation number is CCTCC NO: C202212. The sheep embryo fibroblast line can be used for separation and culture of bovine and sheep pox viridae viruses such as LSDV, GTPV, SPPV and ORFV, has the characteristics of short proliferation time and high virus titer, and can avoid the risk that other viruses are polluted by viruses generated by primary cell separation.

The invention provides an improved method for separating liver parenchymal cells from non-parenchymal cells of a mouse with a non-alcoholic fatty liver disease induced by a high fat diet. According to the method, the NAFLD mouse model is successfully constructed by feeding a high-fat feed containing 60% of fat for 12-16 weeks. An improved two-step collagenase perfusion technology is adopted, a low-concentration enzyme solution is combined, and digestion time is accurately controlled, so that cell damage is effectively reduced. The efficient separation and purification of parenchymal hepatic cells and non-parenchymal cells are realized through a Percoll solution with a specific proportion by utilizing a density gradient centrifugation method. Flow cytometry and molecular biological technical verification show that the separated cells are high in survival rate and excellent in purity. The method provides a high-quality primary cell material for research on NAFLD pathogenesis and drug target discovery, and has important application value.

The present application relates to the field of cell separation, especially to the flexible monomer soaking separation device and soaking separation method for mottled bean cotyledon cell separation. When the multi-purpose plug (6) is properly moved upwards, the gap (20) appears; the upper support (1) fixes the sieve fixing support (9) downwards, and the sieve (15) is fixed in the middle of the sieve fixing support (9); the heating device (12) is an electric heating device. The present application innovatively integrates soaking, water-bean separation and extrusion crushing separation in one device, and can complete all the above processes by using one device; the demand for workstations is small, the land occupation is small, the cost is extremely low, and the device can be packaged and sold as an integrated device. 1. Multi-station integration; 2. The multi-purpose container (8) is used as a suspended soaking heating device, which directly falls after soaking is completed, realizing water-bean separation; 3. The lifting structure can be used as a gap blocking device and also as a downward extrusion device.

A semiconductor device includes: a cell vertical active pattern and a peripheral vertical active pattern at the same vertical height; an on-cell extension source / drain pattern and a cell contact plug sequentially stacked on the cell vertical active pattern; a peripheral upper extension source / drain pattern and a peripheral contact plug sequentially stacked on the peripheral vertical active pattern; a cell isolation pattern on a side surface of the on-cell extension source / drain pattern and a side surface of the cell contact plug; a peripheral isolation pattern on a side surface of the peripheral upper extended source / drain pattern and a side surface of the peripheral contact plug; and an upper interconnect on the peripheral contact plug and the peripheral isolation pattern. A first distance between a vertical height of an upper end of the cell contact plug and a vertical height of an upper end of the cell isolation pattern is different from a second distance between a vertical height of an upper end of the peripheral contact plug and a vertical height of an upper end of the peripheral isolation pattern.

The invention discloses application of IMR-1A in isolated culture of bovine embryonic stem cells, and belongs to the technical field of embryo engineering. When IMR-1A with the final concentration of 0.5-2 [mu] M is added into a conventional bovine embryonic stem cell culture solution, the establishment efficiency of the bovine embryonic stem cells can be effectively improved to 80%, and the pluripotency of the separated stem cells can be effectively maintained. Compared with a conventional bovine embryonic stem cell culture solution, after the IMR-1A is added, the expression levels of pluripotent related genes and proteins of the stem cells are remarkably improved. The research result is of great significance in improving a breeding system, improving genetic screening efficiency and breaking through germplasm breeding bottlenecks of cattle.

The invention belongs to the technical field of biology, and particularly relates to an isolated culture method of dairy cow fat-derived macrophages and application of the isolated culture method. According to the method for separating and culturing the dairy cow fat-derived macrophages, the dairy cow fat tissue serves as a research object, the ATM is separated through the immunomagnetic bead sorting technology, the treatment mode of the dairy cow fat tissue and the cell separation and culture method are optimized, operability is high, the method is simple, the physiological state is stable, and the ATM is promoted to be differentiated to the functional M1 / M2 phenotype. The invention provides a reliable cell model for research on fat metabolism, inflammation regulation and related diseases of dairy cows. The popularization and application of the invention are expected to provide new tools and ideas for health research and disease prevention and treatment of dairy cows, and the sustainable development of the breeding industry is promoted.

In alternative embodiments, provided herein are methods for isolating primary retinal cells obtained or isolated from a human sample. In alternative embodiments, provided are compositions and methods for treating, ameliorating or preventing a retinal disease or condition; improving a photopic (day light) vision; for improving correcting visual acuity, improving macular function, improving a visual field, or improving scotopic (night) vision by administration of retinal progenitor cells or regenerating a macular and / or a scotopic visual function.