33 results about "Cells isolation" patented technology

A system and method for early detection of thermal runaway and electrochemical degradation in battery cells and packs includes a sensor with at least two conductive electrodes separated by a sub-millimeter gap and biased by a controlled voltage. When thermal runaway byproducts enter the gap, current between the electrodes is altered to produce a measurable signal, conditioned by onboard or remote circuitry through amplification / filtering to generate a processed signal. This signal is transmitted to a battery control system to initiate protective actions (e.g., shutdown, cooling activation, or cell isolation). The sensor may be implemented on a printed circuit board or as discrete electrodes and may include a gas-permeable filter that admits vapors and fine aerosols while blocking liquids and debris. The disclosed system enables real-time, sub-second detection of early thermal runaway precursors before temperature rise or combustion occurs, enhancing safety and reliability of lithium-ion and other electrochemical energy storage systems.

A system and method for early detection of thermal runaway and electrochemical degradation in battery cells and packs includes a sensor with at least two conductive electrodes separated by a sub-millimeter gap and biased by a controlled voltage. When thermal runaway byproducts enter the gap, current between the electrodes is altered to produce a measurable signal, conditioned by onboard or remote circuitry through amplification / filtering to generate a processed signal. This signal is transmitted to a battery control system to initiate protective actions (e.g., shutdown, cooling activation, or cell isolation). The sensor may be implemented on a printed circuit board or as discrete electrodes and may include a gas-permeable filter that admits vapors and fine aerosols while blocking liquids and debris. The disclosed system enables real-time, sub-second detection of early thermal runaway precursors before temperature rise or combustion occurs, enhancing safety and reliability of lithium-ion and other electrochemical energy storage systems.

The invention relates to a cell separating element (10) for arrangement in an intermediate space (42) between two battery cells (36) of a cell stack (16) arranged adjacent to one another in a stacking direction (y), wherein the cell separating element (10) has a first flexible outer wall (12) and a second flexible outer wall (14) which are at least partially connected to one another circumferentially at the edges, and wherein an interior space (30) of the cell separating element (10) is located between the first and second outer walls (12, 14). The cell separation element (10) comprises an intermediate plate (18) which is arranged between the first and second outer wall (12, 14), on which at least one first resilient support element (20a) is arranged, which resiliently supports the intermediate plate (18) against the first outer wall (12), and on which at least one second resilient support element (20b) is arranged, which resiliently supports the intermediate plate (18) against the second outer wall (14).

Novel nucleic acid compositions, vector systems, modified cells, isolated peptides, isolated nucleic acid sequences and pharmaceutical compositions that encode or express T cell receptor components directed against Jchain are provided herein. These novel components may be used to enhance an immune response in a subject diagnosed with a B cell associated disease or condition. Associated methods for treating such subjects are also provided herein.

The present invention provides an AutoCell Platform (ACP), an advanced, fully automated, functionally closed system designed to revolutionize the manufacturing of genetically modified cell therapies. This innovative platform integrates automation, closed-loop processing, and novel technologies to address the inefficiencies and high costs of traditional methods. Key features include automated centrifugal seeding for enhanced genetic modification efficiency, microbubble-assisted cell selection for precise cell isolation, and a modular design for scalable amplification across clinical and research environments. By reducing production timelines from 30-40 days to less than 3 days and orders of magnitude in cost, the ACP enables instant manufacturing, decentralization, and broader accessibility. Advanced quality control measures and standardized design ensure compliance and consistent therapeutic outcomes. The ACP supports a variety of applications from CAR-T therapies to regenerative medicine, representing a transformative leap in global accessibility of precision medicine and life-saving cell therapies.

The present disclosure relates to novel Spodoptera frugiperda cell lines free of Sf9 rhabdovirus, methods for making and methods for using the cell lines. In one aspect, the method includes single cell isolation to obtain a cloned cell line and to identify deletion of Sf9 rhabdovirus. In one aspect, the disclosure provides novel cell lines and kits comprising the cell lines. In one aspect, the disclosure provides a method for producing a biological product using the cell line.

The present invention belongs to the technical field of biomedicine. Disclosed are stem-cell-derived apoptotic extracellular vesicles (ApoEVs) having an iron homeostasis regulatory effect and the use thereof in the preparation of a drug for treating iron deficiency anemia. ApoEVs are extracellular vesicles isolated from apoptotic cells derived from human periodontal ligament stem cells, and can effectively restore low intracellular iron levels induced by deferoxamine mesylate (DFOM), upregulate the expression of iron storage proteins and regulate the levels of iron export proteins, thereby improving cellular iron homeostasis. Moreover, the ApoEVs have good biocompatibility and safety, and provide a new strategy for the treatment of iron deficiency anemia.

The invention belongs to the technical field of biology, and particularly relates to a nucleus pulposus cell separation and culture method. According to the method, a step-by-step enzyme digestion method is adopted, NB6 collagenase is firstly used for primary digestion, and then compound enzyme liquid composed of trypsin and hyaluronidase is adopted for subsequent digestion; and culturing in combination with a culture vessel coated with a specific concentration of extracellular matrix component and a special culture medium. According to the method, on the premise of not depending on an immortalization technology, the primary nucleus pulposus cells with good activity can be efficiently obtained, stable amplification and long-term passage of the primary nucleus pulposus cells in vitro are realized, and specific marker expression and biological functions of the nucleus pulposus cells can be effectively maintained. The invention provides a stable cell source and technical platform for the development of cell therapy products and the research of spinal degenerative diseases.

A functionally closed cell processing platform designed to minimize contamination and automate cell processing. The platform integrates multiple specialized cassettes, including a reagent sample cassette (RSC) for reagent delivery and sample collection, a process fluids cassette (PFC) for waste and buffer management, and a cell processing cassette (CPC) for cell handling, along with a transfer syringe cassette (TSC) for precise fluid transfer. The system features a centrifuge capable of multi-mode operation for cell concentration and mixing, pneumatic controls for fluid and microbubble manipulation, and UV sanitizers to maintain aseptic conditions. Advanced functionalities include optical and thermal sensors for real-time monitoring, automated control of fluid and reagent handling, and a microbubble cell selection system for precise target cell isolation. This innovative platform ensures efficient, contamination-free cell processing, supporting applications in gene therapy, cell manufacturing, and biomedical research.

The present invention relates to a probe primer, a probe and an application for labeling the branchial renal cells of Exopalaemon carinicauda, belonging to the field of aquatic biotechnology. The primer sequences are shown as SEQ.ID NO 1 and SEQ.ID NO 2, and the RNA probe sequence is shown as SEQ.ID NO 3. The present invention also provides a kit for labeling the branchial renal cells of Exopalaemon carinicauda, and the kit contains the RNA probe. The present invention also provides the application of the RNA probe in labeling the branchial renal cells of Exopalaemon carinicauda. The present invention locates the branchial renal cells of Exopalaemon carinicauda through fluorescence in situ hybridization of the NAR6 gene, providing theoretical and technical support for exploring the functions of renal cells and cell isolation and culture research.

A 3D NAND flash memory device and fabricating method thereof, comprises: First, a semiconductor substrate layer is provided, wherein cell isolation structures, channel structures and source lead-out spaces are formed on the semiconductor substrate layer, with the cell isolation structures including cell isolation layers and memory cell-occupied spaces. First dielectric layers and second dielectric layers are sequentially deposited, the first dielectric layer covering at least an exposed surface of the cell isolation structure. The second dielectric layers are then etched to remove all of the second dielectric layers on the side surface and at least part of the second dielectric layers on the second surface, and all of the second dielectric layers on the first surface is retained, with the remaining second dielectric layer forming at least one electron capture layer for memory cell structures. The memory cell structures and electrode structures for the flash memory device are finally fabricated.

The invention relates to the technical field of battery production, and particularly discloses a battery cell isolation film tearing structure which comprises a workbench, a film tearing mechanism is arranged on the workbench, an isolation film body comprises a middle layer and release films on the two sides, easy-to-tear parts are arranged at the two ends of each release film, and the film tearing mechanism is used for adsorbing and loosening the easy-to-tear parts of the release films; the film tearing mechanism comprises two sets of suction cups, the suction cups are connected with an air pump, and the two sets of suction cups face the two easy-to-tear parts of the release film correspondingly. The air pump is arranged on the mounting plate, and the mounting plate is connected with the lifting plate. In the film tearing process, the two sides of a middle layer are pressed through the film pressing roller, the lifting mechanism drives the film tearing mechanism to continue to move upwards, a release film can be separated from the middle layer, in the process, the middle layer can be prevented from being lifted through extrusion of the film pressing roller, the middle layer after film tearing can be neatly arranged on the workbench, and the film tearing efficiency is improved. And when the intermediate layer is subsequently attached to the battery cell, the attached intermediate layer is relatively tidy.

According to the embodiments provided herein, a photovoltaic device may have one or more cells with in-cell isolation.

The invention discloses a culture method of horse colonic epithelial cells. Belongs to the technical field of cell separation culture. According to the method, the horse colonic epithelial cell model is established in vitro by adopting the colonic tissue of the large monogastric herbivorous animal horse for the first time, the method is simple and convenient in procedure and low in cost, the influence of in-vivo complex environment, specific research conditions and treatment on the cell growth state can be avoided, the influence caused by individual differences is avoided, and the method is suitable for large-scale popularization and application. Meanwhile, impurity cells generated in the separation culture process can be effectively removed, so that the separation efficiency of the colonic epithelial cells is improved. The establishment of the in-vitro model of the colon epithelial cells of the horses can provide a basis for the subsequent research on intestinal diseases. Compared with an enzyme digestion method, the method disclosed by the invention has the advantages that the primary cells can reach about 80% after being cultured for 13 days, and passage operation can be carried out.

Disclosed herein are methods, systems, and kits for separating a cell in an aqueous sample, cell comprising a primary binding member, a magnetic particle comprising a particle binding site, a secondary binding member comprising a primary binding member binding site reactive to the primary binding member, and a binding site reactive to the particle binding site. The methods and systems expose the aqueous sample to the secondary binding member and to the magnetic particle to form a selected mixture including: a magnetically tagged cell of the secondary binding member bound to the particle binding site of the magnetic particle, and the secondary binding member bound to the primary binding member of the cell. Further, the selected mixture is placed on a surface of a substrate having an oil and is exposed to a magnetic field, magnetically attracting the magnetically tagged cell to the magnetic field, thereby separating the magnetically tagged cell from the aqueous sample to produce a separated aqueous sample.

The invention provides a sheep embryo fibroblast line and application thereof, and belongs to the technical field of cell biology. The name of the sheep embryo fibroblast line is sheep embryo fibroblast OEF, the sheep embryo fibroblast line is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is January 26, 2022, and the preservation number is CCTCC NO: C202212. The sheep embryo fibroblast line can be used for separation and culture of bovine and sheep pox viridae viruses such as LSDV, GTPV, SPPV and ORFV, has the characteristics of short proliferation time and high virus titer, and can avoid the risk that other viruses are polluted by viruses generated by primary cell separation.

The invention provides an improved method for separating liver parenchymal cells from non-parenchymal cells of a mouse with a non-alcoholic fatty liver disease induced by a high fat diet. According to the method, the NAFLD mouse model is successfully constructed by feeding a high-fat feed containing 60% of fat for 12-16 weeks. An improved two-step collagenase perfusion technology is adopted, a low-concentration enzyme solution is combined, and digestion time is accurately controlled, so that cell damage is effectively reduced. The efficient separation and purification of parenchymal hepatic cells and non-parenchymal cells are realized through a Percoll solution with a specific proportion by utilizing a density gradient centrifugation method. Flow cytometry and molecular biological technical verification show that the separated cells are high in survival rate and excellent in purity. The method provides a high-quality primary cell material for research on NAFLD pathogenesis and drug target discovery, and has important application value.

The invention relates to a multi-foot composite material with a controllable surface topological structure and a preparation method and application thereof, a multi-foot structure grows on the surface of the material, the length of each foot is 50-500nm, and the diameter of each foot is 50-150nm; the preparation method comprises the following steps: reacting in a water-ethanol mixed system, and growing a multi-sufficient structure on the surface of a raw material by taking hexadecyl trimethyl ammonium bromide as a structure-directing agent, 1, 2-bis (triethoxysilyl) ethane as a silicon source and ammonia water as a catalyst; the prepared multi-legged composite material can be applied to the fields of oral administration, strain screening, cell separation and detection and the like. Compared with the prior art, the method has the advantages that the morphology of the surface of the material can be regulated and controlled, and the method has important practical application value and is a universal method for regulating and controlling the interaction force of the material-biological interface.

Novel nucleic acid compositions, vector systems, modified cells, isolated peptides, isolated nucleic acid sequences and pharmaceutical compositions that encode or express T cell receptor components directed against haematopoietic-restricted minor histocompatibility antigens (MiHAs) are provided herein. These novel components may be used in the treatment of a subject having a haematological malignancy, particularly after the subject has undergone allogeneic stem cell transplantation (alloSCT). Associated methods for treating such subjects are also provided herein.

The present application relates to the field of cell separation, especially to the flexible monomer soaking separation device and soaking separation method for mottled bean cotyledon cell separation. When the multi-purpose plug (6) is properly moved upwards, the gap (20) appears; the upper support (1) fixes the sieve fixing support (9) downwards, and the sieve (15) is fixed in the middle of the sieve fixing support (9); the heating device (12) is an electric heating device. The present application innovatively integrates soaking, water-bean separation and extrusion crushing separation in one device, and can complete all the above processes by using one device; the demand for workstations is small, the land occupation is small, the cost is extremely low, and the device can be packaged and sold as an integrated device. 1. Multi-station integration; 2. The multi-purpose container (8) is used as a suspended soaking heating device, which directly falls after soaking is completed, realizing water-bean separation; 3. The lifting structure can be used as a gap blocking device and also as a downward extrusion device.

A semiconductor device includes: a cell vertical active pattern and a peripheral vertical active pattern at the same vertical height; an on-cell extension source / drain pattern and a cell contact plug sequentially stacked on the cell vertical active pattern; a peripheral upper extension source / drain pattern and a peripheral contact plug sequentially stacked on the peripheral vertical active pattern; a cell isolation pattern on a side surface of the on-cell extension source / drain pattern and a side surface of the cell contact plug; a peripheral isolation pattern on a side surface of the peripheral upper extended source / drain pattern and a side surface of the peripheral contact plug; and an upper interconnect on the peripheral contact plug and the peripheral isolation pattern. A first distance between a vertical height of an upper end of the cell contact plug and a vertical height of an upper end of the cell isolation pattern is different from a second distance between a vertical height of an upper end of the peripheral contact plug and a vertical height of an upper end of the peripheral isolation pattern.

Embodiments herein are directed to a system and a method of selectively manipulating magnetically-barcoded materials from background magnetic materials. Magnetic barcodes include layers of magnetic anisotropy. These are then manipulated by a magnetic system that can drive spatio-temporal magnetic fields that can “match” a barcode to drive a specific interaction, thereby providing a “lock-key” interaction. This technique is able to selectively manipulate magnetically-barcoded materials, and can have applications across a variety of magnetic systems such as cell separation, drug delivery, valves, and motors.

Novel nucleic acid compositions, vector systems, modified cells, isolated peptides, isolated nucleic acid sequences and pharmaceutical compositions that encode or express T cell receptor components directed against haematopoietic-restricted minor histocompatibility antigens (MiHAs) are provided herein. These novel components may be used in the treatment of a subject having a haematological malignancy, particularly after the subject has undergone allogeneic stem cell transplantation (alloSCT). Associated methods for treating such subjects are also provided herein.

The present invention relates to compositions suitable for cell detachment, comprising a polypeptide having protease activity. The invention further relates to polypeptides having protease activity, polynucleotides encoding said polypeptides, nucleic acid constructs and expression vectors comprising said polynucleotides, recombinant host cells comprising said nucleic acid constructs or expression vectors, methods for producing said polypeptides, granules comprising said polypeptides, fermentation broth formulations comprising said polypeptides, and methods and uses utilizing said polypeptides.

The invention discloses application of IMR-1A in isolated culture of bovine embryonic stem cells, and belongs to the technical field of embryo engineering. When IMR-1A with the final concentration of 0.5-2 [mu] M is added into a conventional bovine embryonic stem cell culture solution, the establishment efficiency of the bovine embryonic stem cells can be effectively improved to 80%, and the pluripotency of the separated stem cells can be effectively maintained. Compared with a conventional bovine embryonic stem cell culture solution, after the IMR-1A is added, the expression levels of pluripotent related genes and proteins of the stem cells are remarkably improved. The research result is of great significance in improving a breeding system, improving genetic screening efficiency and breaking through germplasm breeding bottlenecks of cattle.

The utility model relates to the field of biomedical engineering, in particular to a multi-channel cell separation device based on magnetic nanoparticles, which comprises an incubator, a first input pipe and a second input pipe communicated with the incubator, a plurality of channel pipes communicated with the incubator, a temporary storage pipe communicated with each channel pipe, and a plurality of magnetic nanoparticles communicated with the temporary storage pipe. One end, far away from the channel pipe, of the temporary storage pipe is arranged upwards, a coil is arranged on the temporary storage pipe, one end, far away from the incubator, of the channel pipe is provided with a first control valve, one end, far away from the channel pipe, of the temporary storage pipe is provided with a second control valve, and the second control valve is communicated with a collecting chamber; and a valve is arranged between each channel pipe and the incubator. The cell separation device has the effects that the cell separation operation is simplified, and the cell separation efficiency is improved.

A functionally closed cell processing platform designed to minimize contamination and automate cell processing. The platform integrates multiple specialized cartridges, including a reagent sample cartridge (RSC) for reagent delivery and sample collection, a process fluid cartridge (PFC) for waste and buffer management, a cell processing cartridge (CPC) for cell manipulation, and a transfer syringe cartridge (TSC) for precise fluid transfer. System features include a centrifuge capable of multi-modal operation for cell concentration and mixing, pneumatic controls for fluid and microbubble manipulation, and a UV sterilizer for maintaining sterile conditions. Its advanced functionalities include optical and thermal sensors for real-time monitoring, automated control over fluid and reagent processing, and a microbubble cell selection system for precise target cell isolation. This innovative platform ensures efficient, contamination-free cell processing, supporting applications in gene therapy, cell manufacturing, and biomedical research.

The invention belongs to the technical field of biology, and particularly relates to an isolated culture method of dairy cow fat-derived macrophages and application of the isolated culture method. According to the method for separating and culturing the dairy cow fat-derived macrophages, the dairy cow fat tissue serves as a research object, the ATM is separated through the immunomagnetic bead sorting technology, the treatment mode of the dairy cow fat tissue and the cell separation and culture method are optimized, operability is high, the method is simple, the physiological state is stable, and the ATM is promoted to be differentiated to the functional M1 / M2 phenotype. The invention provides a reliable cell model for research on fat metabolism, inflammation regulation and related diseases of dairy cows. The popularization and application of the invention are expected to provide new tools and ideas for health research and disease prevention and treatment of dairy cows, and the sustainable development of the breeding industry is promoted.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a grape stem cell separation, culture and identification method based on stem tip meristem. Aiming at the problems that the existing grape stem cell separation steps are complicated, the induction efficiency is low and a standardized identification method is lacked, the method is realized by the following steps: collecting terminal buds or immature embryos of grapes, sterilizing, and screening and separating stem tip meristem tissues by using a sucrose solution; inoculating to an induction culture medium (containing gibberellin, kinetin, indoleacetic acid and other components), carrying out dark culture at 22 DEG C for 20-30 hours, and carrying out two-stage filtration to obtain the embryonic stem cells; then carrying out enlarged culture in a liquid culture medium (containing 2, 4-dichlorphenoxyacetic acid, gibberellin, kinetin and the like) for 20 days; and finally, detecting the expression quantity of WUS and CLV3 genes through qRT-PCR (quantitative real-time polymerase chain reaction), and verifying the characteristics of the stem cells by taking EF1a as an internal reference. According to the method, the induction time is remarkably shortened, the pollution risk is reduced, a molecular level identification standard is established, the method is suitable for the field of grape germplasm resource preservation and breeding, and an efficient and universal solution is provided for a plant stem cell technology.