86 results about "Cells isolation" patented technology

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22/C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to Outage notification system features, and corresponding methodology and apparatus subject matters, both at the network and device level.

The invention discloses a method and an automatic instrument device for enriching and extracting target cells and separating single cells by using a positive magnetic bead method and performing immunity and molecular biology identification and analysis on single cells. By the method and the instrument device, the on/off of a capture magnet and a release magnet is controlled by various methods to complete target cell searching, capturing, cleaning and releasing operation once or for multiple times, so that the target cell detection sensitivity and stability are improved. When the capture magnet searches and captures the target cells, the search line is circular, square, comb-shaped, S-shaped or U-shaped. In addition, the captured substances are filtered, most free micro magnetic beads are removed, the purity of the product is further improved and the product can be used as a good experimental material. By combining a special filter and the adsorption of the capture magnet, more than 95 percent of free micro magnetic beads can be effectively removed. Meanwhile, the types of the single cells are identified and biological characteristics of the single cells are analyzed by an immunofluorescence staining method and a method in molecular biology, and effective biological indexes are provided for clinical diagnosis and treatment of cancers.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22/C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to broadcast acknowledgement features.

Systems and methods for generating a high voltage pulse. A series of voltage cells are connected such that charging capacitors can be charged in parallel and discharged in series. Each cell includes a main switch and a return switch. When the main switches are turned on, the capacitors in the cells are in series and discharge. When the main switches are turned off and the return switches are turned on, the capacitors charge in parallel. One or more of the cells can be inactive without preventing a pulse from being generated. The amplitude, duration, rise time, and fall time can be controlled with the voltage cells. Each voltage cell may also include a balance network to match the stray capacitance seen by each voltage cell. Droop compensation is also enabled. Isolation diodes ensure that a discharge current can bypass inoperable voltage cells.

Embodiments of the present invention provide methods for fabricating a solar cell on a substrate that have proportionally reduced current to minimize or reduce the likelihood of shading of a portion of the solar cell causing damage to the formed device. In one embodiment, a method for fabricating a series of solar cell arrays on a substrate includes providing a substrate having a TCO layer formed thereon, forming a first plurality of vertical scribing lines and a first plurality of horizontal scribing lines in the TCO layer, forming a film stack and a back metal layer on the scribed TCO layer, and forming a second plurality of the horizontal scribing lines in the film stack and the back metal layer, wherein the second plurality of horizontal scribing lines comprise pairs of scribing lines formed adjacent to each respective one of the first plurality of the horizontal scribing lines formed in the TCO layer.

The present invention provides a blood cell separation membrane for separating blood into serum or plasma and blood cells while preventing hemolysis of the blood cells. Such a blood cell separation membrane can be obtained by impregnating a porous membrane for separating blood cells from blood supplied thereto with a solution containing a hydrophobic aminocarboxylic acid, a protein derived from silk, Tris, TES, ε-aminohexanoic acid, tranexamic acid, or heparin and then drying the membrane.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22/C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters concerning the handling of Beacon Requests and Registered State bit resolving to avoid circular routes.

The present invention is apllied in fields like biological medicine and tissue engineering. A fluid control system in a cell isolation and culture system is used to automatically process sample preparation, circulating tumor cell (CTC) isolation, plate changing and cell culturing. By using the present invention, time and labor are saved; moreover, the present invention has a small size and is easily carried.

The invention discloses a cell-culture micro-fluidic chip with a self-antibacterial function. The cell-culture micro-fluidic chip comprises a flexible polymer surface-layer thin film, an upper-layer chip, a lower-layer chip, a micro valve and a guide pipe, wherein the flexible polymer surface-layer thin film is provided with a cell culture chamber, a culture-solution guide-pipe through hole, a waste-culture-solution micro-valve through hole, a buffer-solution guide-pipe through hole and an enrichment-cavity micro-valve through hole; the upper-layer chip is provided with a cell-culture-chamber bottom groove, a waste-culture-solution micro-valve bottom groove, a waste-culture-solution outlet, a buffer-solution guide-pipe bottom groove, a cell-separation flow channel, an enrichment cavity, an enrichment-cavity micro-valve bottom groove and a waste-separation-solution outlet; the upper surface of the lower-layer chip is paved with driving electrodes corresponding to the positions of the cell-culture-chamber bottom groove, the cell-separation flow channel and the enrichment cavity; the guide pipe comprises a culture-solution guide pipe and a buffer-solution guide pipe; and the micro valve comprises a waste-culture-solution micro valve and an enrichment-cavity micro valve. The cell-culture micro-fluidic chip disclosed by the invention has the advantages of minimization, high efficiency, low cost, repeatable use and self-antibacterial function and the like, and has large use prospect in the biomedicine.

The embodiment of the invention provides a micro-fluidic chip for isolating and capturing a single cell. The micro-fluidic chip comprises a chip substrate; the chip substrate is provided with a sampleinjection hole and a plurality of sampling holes; the sampling injection hole is located in the center of the chip substrate, wherein the sample injection hole communicates with at least two groups of multistage flow channels, each group of multistage flow channel comprises a main flow channel, a first branch flow channel and a plurality of second branch flow channels; one end of the main flow channel communicates with the sample injection hole, and the other end of the main flow channel communicates with the first branch flow channel; and the plurality of second branch flow channel are symmetrically arranged at two sides of the first branch flow channel, one end of each of the plurality of second branch flow channels communicates with the first branch flow channel, the other end of eachof the second branch flow channels is connected with at least one of the sampling holes, and each of the sampling holes communicates with one of the second branch flow channels. By using the micro-fluidic chip, the single chip can be rapidly and simply isolated and captured with low cost and low threshold. The invention further provides a preparation method and application of the micro-fluidic chip.

A method for analyzing single cells by mass spectrometry includes the steps of providing a plurality of cells in a liquid medium and placing the cells and liquid medium in a single cell isolation and ejection system. Liquid medium containing a single cell is released from the single cell isolation and ejection system. The liquid medium and single cell are captured in a capture probe containing a flowing capture probe solvent. The cell is lysed by a lysis inducer in the capture probe to disperse single cell components into the medium. The lysed single cell components are transported to a mass spectrometer, where the lysed single cell components entering the mass spectrometer are spatially and temporally separated from any dispersed components of another single cell from the sample entering the mass spectrometer. Mass spectrometry is conducted on the lysed single-cell components. A system for analyzing single cells by mass spectrometry is also disclosed.

A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out.

The invention discloses a microfluidic chip for accurately controlling and pairing single particles. The chip comprises a channel layer and a control layer. The channel layer comprises a plurality ofunits for capturing and transferring single particles, and each unit consists of a capture flow channel, a capture chamber, a capture gap, a transfer flow channel, a pairing chamber and a pairing gap.The control layer is positioned below the capture flow channel and a pairing flow channel, is vertical to the capture flow channel and the pairing flow channel, and is isolated by a diaphragm. The chip can efficiently and accurately control the capture and transfer of single particles, and can realize high-throughput and high-efficiency single-particle pairing after different rounds of single-particle capture and transfer, and the number and the type of the paired particles are controllable. The chip can be widely applied to single-cell isolation and culture, single-cell heterogeneity analysis, multi-cell co-culture, multi-cell interaction, potential mechanism disclosure and the like.

The present invention, relates to novel methods of isolating and expanding pluripotent stem cells, including multi-lineage stress enduring (MUSE) cells.

Particles, such as cells, are isolated for conducting single-particle measurement. Isolation of rare cells, such as circulating tumor cells (CTCs), from blood is technically challenging because they are small in numbers. An integrated microfluidic biochip, dubbed as CTC chip, was designed and fabricated for conducting tumor cell isolation. As CTCs are usually multidrug resistance (MDR), the effect of MDR inhibitors on chemotherapeutic drug accumulation in the isolated single tumor cell is measured. In this invention, label-free isolation of the rare tumor cells was conducted based on cell size difference. The major advantages of the CTC chip are the ability of fast cell isolation, followed by multiple rounds of single-cell measurement, suggesting a potential assay for detecting the drug responses based on the liquid biopsy of cancer patients.