93 results about "Cells isolation" patented technology

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and / or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to Outage notification system features, and corresponding methodology and apparatus subject matters, both at the network and device level.

The invention discloses a method and an automatic instrument device for enriching and extracting target cells and separating single cells by using a positive magnetic bead method and performing immunity and molecular biology identification and analysis on single cells. By the method and the instrument device, the on / off of a capture magnet and a release magnet is controlled by various methods to complete target cell searching, capturing, cleaning and releasing operation once or for multiple times, so that the target cell detection sensitivity and stability are improved. When the capture magnet searches and captures the target cells, the search line is circular, square, comb-shaped, S-shaped or U-shaped. In addition, the captured substances are filtered, most free micro magnetic beads are removed, the purity of the product is further improved and the product can be used as a good experimental material. By combining a special filter and the adsorption of the capture magnet, more than 95 percent of free micro magnetic beads can be effectively removed. Meanwhile, the types of the single cells are identified and biological characteristics of the single cells are analyzed by an immunofluorescence staining method and a method in molecular biology, and effective biological indexes are provided for clinical diagnosis and treatment of cancers.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to broadcast acknowledgement features.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to Downlink routing mechanisms.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to real time clock distribution and recovery.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to cell size management.

In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

Embodiments of the present invention provide methods for fabricating a solar cell on a substrate that have proportionally reduced current to minimize or reduce the likelihood of shading of a portion of the solar cell causing damage to the formed device. In one embodiment, a method for fabricating a series of solar cell arrays on a substrate includes providing a substrate having a TCO layer formed thereon, forming a first plurality of vertical scribing lines and a first plurality of horizontal scribing lines in the TCO layer, forming a film stack and a back metal layer on the scribed TCO layer, and forming a second plurality of the horizontal scribing lines in the film stack and the back metal layer, wherein the second plurality of horizontal scribing lines comprise pairs of scribing lines formed adjacent to each respective one of the first plurality of the horizontal scribing lines formed in the TCO layer.

The present invention provides a blood cell separation membrane for separating blood into serum or plasma and blood cells while preventing hemolysis of the blood cells. Such a blood cell separation membrane can be obtained by impregnating a porous membrane for separating blood cells from blood supplied thereto with a solution containing a hydrophobic aminocarboxylic acid, a protein derived from silk, Tris, TES, ε-aminohexanoic acid, tranexamic acid, or heparin and then drying the membrane.

A battery system includes a multi-cell battery pack in electrical communication with a load. The voltages of each cell are individually monitored by the microcontroller, such as with a high-impedance input terminal. Across each of the cells is a transistor-resistor combination such that by providing a voltage to the gate of each of the transistors, a short-circuit is created through the corresponding cell thereby providing an additional current drain on the cell. More specifically, by turning on the transistor, a short-circuit current (ISQ1) is drawn from the cell through resistor (R1) to provide for the isolated discharge of the specific cell. By selectively measuring each of the cells in a multi-cell battery pack to determine if any of the cells are over-voltage, and if so, by increasing the current drain on that specific cell, the overall maximum amount of energy can be transferred to a load across the battery pack. Moreover, this selectively isolation and discharge provides a mechanism for maintaining a constant charge across all batteries in a multi-cell batter pack.

A semiconductor device is provided, which prevents a case where the widths of word lines become uneven because of a stress developing at the border between a memory cell area and a peripheral circuit area. The semiconductor device 1 has a semiconductor substrate 2 on which a memory cell area MC defined by a peripheral isolation region 3c. The memory cell area MC has multiple cell active regions k defined by multiple cell isolation regions 3a, 3b. Guard active regions GLa, GLb made of the semiconductor substrate are disposed in the border between the memory cell area MC and the peripheral isolation region 3c to separate the memory cell isolation regions 3a, 3b from the peripheral isolation region 3c.

The present disclosure relates to devices, systems, and methods for single cell isolation and analysis. In particular, the present disclosure relates to systems and methods for isolating single cells present at low numbers.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters concerning the handling of Beacon Requests and Registered State bit resolving to avoid circular routes.

The invention provides a mesenchymal stem cell serum-free medium. The mesenchymal stem cell serum-free medium is prepared from, by volume, 94-97 parts of DMEM / F12, 2-5 parts of human blood platelet lysate and 1 part of non-essential amino acid. The invention further provides application of the medium and isolation and cultivation methods of the mesenchymal stem cell serum-free medium. The serum-free medium can be used for effectively separating and cultivating mesenchymal stem cells derived from placentae and umbilical cords, the effects are superior to commercially available media, the cost is low, the safety is high, and the application prospect is good.

Systems and methods for generating a high voltage pulse. A series of voltage cells are connected such that charging capacitors can be charged in parallel and discharged in series. Each cell includes a main switch and a return switch. When the main switches are turned on, the capacitors in the cells are in series and discharge. When the main switches are turned off and the return switches are turned on, the capacitors charge in parallel. One or more of the cells can be inactive without preventing a pulse from being generated. The amplitude, duration, rise time, and fall time can be controlled with the voltage cells. Each voltage cell may also include a balance network to match the stray capacitance seen by each voltage cell. Droop compensation is also enabled. Isolation diodes ensure that a discharge current can bypass inoperable voltage cells.

The invention relates to a multifunctional bioreactor for cell sorting and cell culture in vitro. Said bioreactor comprises five main elements, including an adjustable magnetic field, a multifunctional cell supporting system, a protective perfusion system and a computerized control system. Said methods are for the application of the said bioreactor. The said bioreactor has the functions of cell expansion, cell directed differentiation and cell separation (sorting). It allows its all functions carried out in one reaction chamber.

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and / or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

The invention discloses a Fe3O4-Au magnetic nano composite material, and a preparation method and an application thereof. The preparation method of the Fe3O4-Au magnetic nano composite material comprises the following steps: preparing oil-phase Fe3O4 nano particles; taking 2,3-dimercaptosuccinic acid as a phase transfer modifier, thus obtaining water-soluble Fe3O4 nano particles; and adding a reducing agent and taking chloroauric acid as the raw material to synthesize the Fe3O4-Au magnetic nano composite material through one step in the aqueous solution of Fe3O4. The preparation method is characterized in that small-particle size gold nano particles can be generated on the surface of the Fe3O4 transformed into the water phase from the oil phase through one step, the gold nano particles do not need to be extra synthesized and purified, and therefore, the experiment step is simplified and the particles obtained are even in size. In the meantime, the Fe3O4-Au magnetic nano composite material obtained by the preparation method can be applied to catalysis, biological detection, cell separation and targeted drug delivery.

The present technology relates to protocols relative to utility meters associated with an open operational framework. More particularly, the present subject matter relates to protocol subject matter for advanced metering infrastructure, adaptable to various international standards, while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field. The present subject matter supports meters within an ANSI standard C12.22 / C12.19 system while economically supporting a 2-way mesh network solution in a wireless environment, such as for operating in a residential electricity meter field, all to permit cell-based adaptive insertion of C12.22 meters within an open framework. Cell isolation is provided through quasi-orthogonal sequences in a frequency hopping network. Additional features relate to apparatus and methodology subject matters relating to crystal drift compensation in a mesh network.

The present invention is apllied in fields like biological medicine and tissue engineering. A fluid control system in a cell isolation and culture system is used to automatically process sample preparation, circulating tumor cell (CTC) isolation, plate changing and cell culturing. By using the present invention, time and labor are saved; moreover, the present invention has a small size and is easily carried.

The invention discloses a cell-culture micro-fluidic chip with a self-antibacterial function. The cell-culture micro-fluidic chip comprises a flexible polymer surface-layer thin film, an upper-layer chip, a lower-layer chip, a micro valve and a guide pipe, wherein the flexible polymer surface-layer thin film is provided with a cell culture chamber, a culture-solution guide-pipe through hole, a waste-culture-solution micro-valve through hole, a buffer-solution guide-pipe through hole and an enrichment-cavity micro-valve through hole; the upper-layer chip is provided with a cell-culture-chamber bottom groove, a waste-culture-solution micro-valve bottom groove, a waste-culture-solution outlet, a buffer-solution guide-pipe bottom groove, a cell-separation flow channel, an enrichment cavity, an enrichment-cavity micro-valve bottom groove and a waste-separation-solution outlet; the upper surface of the lower-layer chip is paved with driving electrodes corresponding to the positions of the cell-culture-chamber bottom groove, the cell-separation flow channel and the enrichment cavity; the guide pipe comprises a culture-solution guide pipe and a buffer-solution guide pipe; and the micro valve comprises a waste-culture-solution micro valve and an enrichment-cavity micro valve. The cell-culture micro-fluidic chip disclosed by the invention has the advantages of minimization, high efficiency, low cost, repeatable use and self-antibacterial function and the like, and has large use prospect in the biomedicine.

The invention belongs to the technical field of nanometer magnetic materials used for biology, relates to a nanometer magnetic particle, particularly to an immune nanometer cellulose magnetic liposome and a preparation method thereof. The immune nanometer cellulose magnetic liposome adopts a core-shell structure; the inner core adopts a magnetic material, Fe3O4; a middle layer adopts the mixed material of the liposome and the nanometer cellulose; an outermost layer adopts an antibody wrapping the middle layer; the quality content of the nanometer cellulose is 0.1%-50%; the quality content of the liposome composition is 0.5%-90%; the quality content of the magnetic material is 0.1%-50%. The immune nanometer cellulose magnetic liposome provided by the invention is excellent in biocompatibility; through coupling with the aglucon inside the bioactive substance, the immune nanometer cellulose magnetic liposome can identify and be combined with corresponding antigen, antibody or nucleic acid and the like, so as to rapidly conduct separation and enrichment in an external magnetic field; the immune nanometer cellulose magnetic liposome can be used for detection, separation and purification of biomaterial and has a broad application prospect in biological areas of cell separation, immobilized enzyme, targeted drug, immune detection and the like.

The embodiment of the invention provides a micro-fluidic chip for isolating and capturing a single cell. The micro-fluidic chip comprises a chip substrate; the chip substrate is provided with a sampleinjection hole and a plurality of sampling holes; the sampling injection hole is located in the center of the chip substrate, wherein the sample injection hole communicates with at least two groups of multistage flow channels, each group of multistage flow channel comprises a main flow channel, a first branch flow channel and a plurality of second branch flow channels; one end of the main flow channel communicates with the sample injection hole, and the other end of the main flow channel communicates with the first branch flow channel; and the plurality of second branch flow channel are symmetrically arranged at two sides of the first branch flow channel, one end of each of the plurality of second branch flow channels communicates with the first branch flow channel, the other end of eachof the second branch flow channels is connected with at least one of the sampling holes, and each of the sampling holes communicates with one of the second branch flow channels. By using the micro-fluidic chip, the single chip can be rapidly and simply isolated and captured with low cost and low threshold. The invention further provides a preparation method and application of the micro-fluidic chip.

A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out.

The invention discloses a microfluidic chip for accurately controlling and pairing single particles. The chip comprises a channel layer and a control layer. The channel layer comprises a plurality ofunits for capturing and transferring single particles, and each unit consists of a capture flow channel, a capture chamber, a capture gap, a transfer flow channel, a pairing chamber and a pairing gap.The control layer is positioned below the capture flow channel and a pairing flow channel, is vertical to the capture flow channel and the pairing flow channel, and is isolated by a diaphragm. The chip can efficiently and accurately control the capture and transfer of single particles, and can realize high-throughput and high-efficiency single-particle pairing after different rounds of single-particle capture and transfer, and the number and the type of the paired particles are controllable. The chip can be widely applied to single-cell isolation and culture, single-cell heterogeneity analysis, multi-cell co-culture, multi-cell interaction, potential mechanism disclosure and the like.