Pulse leaf cell separation flexible monomer immersion separation device and immersion separation method

Abstract

The present application relates to the field of cell separation, especially to the flexible monomer soaking separation device and soaking separation method for mottled bean cotyledon cell separation. When the multi-purpose plug (6) is properly moved upwards, the gap (20) appears; the upper support (1) fixes the sieve fixing support (9) downwards, and the sieve (15) is fixed in the middle of the sieve fixing support (9); the heating device (12) is an electric heating device. The present application innovatively integrates soaking, water-bean separation and extrusion crushing separation in one device, and can complete all the above processes by using one device; the demand for workstations is small, the land occupation is small, the cost is extremely low, and the device can be packaged and sold as an integrated device. 1. Multi-station integration; 2. The multi-purpose container (8) is used as a suspended soaking heating device, which directly falls after soaking is completed, realizing water-bean separation; 3. The lifting structure can be used as a gap blocking device and also as a downward extrusion device.

Description

Technical Field

[0001] This invention relates to the field of cell separation, and more particularly to a flexible monomer soaking and separation device and method for separating cotyledon cells from mixed beans. Background Technology

[0002] In the process of deep processing and research of mixed beans, after removing the seed coat, at least two steps are required. The first step is to soak the beans in a solution while heating them. The second step is to press them.

[0003] This process involves soaking the beans, separating the beans from the water, and soft crushing the beans to achieve cell separation.

[0004] Three different sets of equipment are needed.

[0005] Combination Figure 1 The existing technology shown is convenient for soaking during experiments, but the transfer of beans is a major problem during pilot testing.

[0006] Pilot-scale testing from the laboratory requires significant costs. In other words, the shortcomings of existing technologies are: excessive workstation requirements; high costs; and excessive floor space requirements.

[0007] A search of the Chinese patent database for "mixed beans and cells and separation" yielded no results. Summary of the Invention

[0008] Purpose of the invention: To provide a more effective flexible monomer soaking and separation device and method for separating cotyledon cells of mixed beans, the specific purpose of which is described in the specific implementation section for several substantial technical effects.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: A flexible monomer soaking and separation device for separating cotyledon cells of mixed beans, characterized in that... The soaking and separating device includes an upper support 1, on which water inlet 2 and bean inlet 3 are arranged; The multipurpose container 8 is fixed to the two side walls of the upper bracket 1 by the multipurpose container fixing shaft 13; A lifting structure is arranged downwards on the upper support 1. The lifting structure has a lifting shaft 4 extending out, which is connected to a multi-purpose plug 6. The multi-purpose plug 6 has a conical surface 5 on its upper part. The multi-purpose plug 6 can move up and down in the multi-purpose container 8. The bottom of the inner wall of the multi-purpose container 8 is provided with a side wall inner protrusion 14. When the multi-purpose plug 6 moves downwards, it can be blocked by the side wall inner protrusion 14 to prevent it from falling further and thus limit its position. When the edge of the multi-purpose plug 6 fits the side wall inner protrusion 14, the bottom of the multi-purpose container 8 can be relatively closed. When the multi-purpose plug 6 moves upwards appropriately, a gap 20 can appear. The upper support 1 is fixed downwards to the sieve fixing support 9, and the sieve 15 is fixed in the middle of the sieve fixing support 9; A shaft is arranged downward on the upper support 1, and a heating device 12 is arranged at the end of the shaft. The heating device 12 is an electric heating device.

[0010] A further technical solution of the present invention is that the multi-purpose plug 6 is made entirely of rubber.

[0011] A further technical solution of the present invention is that the sieve 15 includes a ring of arc-shaped side arc-shaped soft wall 16.

[0012] A further technical solution of the present invention is that a bottom container 11 is arranged under the sieve 15.

[0013] A further technical solution of the present invention is that a sieve fixing bracket 9 fixes multiple sieves 15 from top to bottom through a sieve fixing shaft 10, and the aperture of the multiple sieves 15 gradually decreases from top to bottom; an auxiliary plug is connected to the bottom position of the multi-purpose plug 6 through a plug linkage shaft 7, and each auxiliary plug corresponds to a sieve 15.

[0014] A further technical solution of the present invention is that the sieve fixing shaft 10 and the plug linkage shaft 7 are arranged in a staggered manner.

[0015] A further technical solution of the present invention is that each additional plug is provided with an additional plug conical surface 18.

[0016] A further technical solution of the present invention is that multiple material trays 21 with multiple layers can be placed in the bottom container 11.

[0017] A further technical solution of the present invention is that a temperature sensor and a level gauge are arranged in the multipurpose container 8.

[0018] A method for separating flexible monomers from cotyledon cells of mixed beans by soaking, characterized in that... Using any of the above-described soaking separation apparatus, the following steps are included: The same container enables soaking, water-bean separation, and crushing separation; Step 1: Soaking: When the edge of the multi-purpose plug 6 fits into the inner protrusion 14 of the side wall, the bottom of the multi-purpose container 8 can be relatively sealed; at this time, water is added to the water inlet 2 and peeled mixed beans are added to the bean inlet 3; then the heating device is activated to soak in warm water. Step 2: Separating water and beans: When the lifting shaft 4 moves upward appropriately, gap 20 appears, at which point the water beans fall from the gap and collect on the sieve 15; Step 3: Extrusion, crushing, and separation: Subsequently, the lifting structure reciprocates, and the multi-purpose plug 6 gradually squeezes the mixed beans, causing them to break down and undergo multi-stage crushing to achieve separation. During multi-stage crushing, the additional plug cone surface 18 guides the flow of broken beans. At this time, water is added to the side for auxiliary rinsing.

[0019] The present invention, employing the above technical solution, has the following advantages over the prior art: Compared to the shortcomings of the prior art, it combines... Figure 1 The existing technology, while convenient for soaking during experiments, presents a significant challenge in the transfer of beans during pilot-scale testing. Conducting pilot-scale testing from the laboratory requires substantial costs. In other words, the shortcomings of the existing technology are: excessive workstation requirements; high costs; and excessive floor space requirements. This patent innovatively integrates soaking, water-bean separation, and extrusion crushing into a single device, enabling all the above processes to be completed with one unit; it requires few workstations, occupies little space, and has extremely low cost, making it suitable for unified packaging and sales as an integrated device.

[0020] This patent has the following outstanding features: 1. Multi-station integration; 2. Innovative use of the multi-purpose container 8 as a suspended soaking and heating device, which falls directly after soaking to achieve water-bean separation; 3. Innovative use of the lifting structure as a gap blocking device and also as a downward squeezing device. Attached Figure Description

[0021] To further illustrate the present invention, the following description is provided in conjunction with the accompanying drawings: Figure 1 This is a diagram illustrating the process steps of the invention. Figure 2 A diagram showing the overall structural implementation of the invention; Figure 3 To create a perspective view for a single sieve; Figure 4 This is a partial structural diagram of the invention; Figure 5 This is a partial structural diagram of the invention; Figure 6 This is a partial structural diagram of the invention; Figure 7 This is a partial structural diagram of the invention; Figure 8 A structural diagram of the material tray for the invention; Figure 9 This is a partial structural diagram of the invention; The components are: 1. Upper support, 2. Water inlet, 3. Bean inlet, 4. Lifting shaft, 5. Conical surface, 6. Multi-purpose plug, 7. Plug linkage shaft, 8. Multi-purpose container, 9. Sieve fixing support, 10. Sieve fixing shaft, 11. Bottom container, 12. Heating device, 13. Multi-purpose container fixing shaft, 14. Side wall inner convex ridge, 15. Sieve, 16. Side arc soft wall, 17. Connecting part, 18. Additional plug conical surface, 19. Additional plug, 20. Gap, 21. Material tray. Detailed Implementation

[0022] The present invention will be further illustrated below with reference to the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," "top," and "bottom," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. In addition, unless otherwise expressly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication of two components. For those skilled in the art, the specific meaning of the above terms in the present invention can be understood according to the specific circumstances.

[0023] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0024] This patent provides multiple parallel solutions; the different descriptions represent improved solutions or parallel solutions based on the basic solution. Each solution has its own unique characteristics. Furthermore, the technical features involved in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other. Fixing methods not described herein can be any type of fixing, such as threaded fixing, bolt fixing, or adhesive bonding.

[0025] Example 1: Referring to all the accompanying drawings; a flexible monomer soaking and separation device for separating cotyledon cells of mixed beans, characterized in that, The soaking and separating device includes an upper support 1, on which water inlet 2 and bean inlet 3 are arranged; The multipurpose container 8 is fixed to the two side walls of the upper bracket 1 by the multipurpose container fixing shaft 13; A lifting structure is arranged downwards on the upper support 1. The lifting structure has a lifting shaft 4 extending out, which is connected to a multi-purpose plug 6. The multi-purpose plug 6 has a conical surface 5 on its upper part. The multi-purpose plug 6 can move up and down in the multi-purpose container 8. The bottom of the inner wall of the multi-purpose container 8 is provided with a side wall inner protrusion 14. When the multi-purpose plug 6 moves downwards, it can be blocked by the side wall inner protrusion 14 to prevent it from falling further and thus limit its position. When the edge of the multi-purpose plug 6 fits the side wall inner protrusion 14, the bottom of the multi-purpose container 8 can be relatively closed. When the multi-purpose plug 6 moves upwards appropriately, a gap 20 can appear. The upper support 1 is fixed downwards to the sieve fixing support 9, and the sieve 15 is fixed in the middle of the sieve fixing support 9; A shaft is arranged downward on the upper support 1, and a heating device 12 is arranged at the end of the shaft. The heating device 12 is an electric heating device.

[0026] The substantive technical effects and implementation process of the technical solution described herein, i.e., its basic functions, are as follows: A flexible monomer soaking and separation method for separating cotyledon cells of mixed beans, characterized in that... Using any of the above-described soaking separation apparatus, the following steps are included: The same container enables soaking, water-bean separation, and crushing separation; Step 1: Soaking: When the edge of the multi-purpose plug 6 fits into the inner protrusion 14 of the side wall, the bottom of the multi-purpose container 8 can be relatively sealed; at this time, water is added to the water inlet 2 and peeled mixed beans are added to the bean inlet 3; then the heating device is activated to soak in warm water. Step 2: Separating water and beans: When the lifting shaft 4 moves upward appropriately, gap 20 appears, at which point the water beans fall from the gap and collect on the sieve 15; Step 3: Extrusion, crushing, and separation: Subsequently, the lifting structure reciprocates, and the multi-purpose plug 6 gradually squeezes the mixed beans, causing them to break down and undergo multi-stage crushing to achieve separation. During multi-stage crushing, the additional plug cone surface 18 guides the flow of broken beans. At this time, water is added to the side for auxiliary rinsing.

[0027] Compared to the shortcomings of existing technologies: "combination" Figure 1The existing technology, while convenient for soaking during experiments, presents a significant challenge in the transfer of beans during pilot-scale testing. Conducting pilot-scale testing from the laboratory requires substantial costs. In other words, the shortcomings of the existing technology are: excessive workstation requirements; high costs; and excessive floor space requirements. This patent innovatively integrates soaking, water-bean separation, and extrusion crushing into a single device, enabling all the above processes to be completed with one unit; it requires few workstations, occupies little space, and has extremely low cost, making it suitable for unified packaging and sales as an integrated device.

[0028] This patent has the following outstanding features: 1. Multi-station integration; 2. Innovative use of the multi-purpose container 8 as a suspended soaking and heating device, which falls directly after soaking to achieve water-bean separation; 3. Innovative use of the lifting structure as a gap blocking device and also as a downward squeezing device.

[0029] Example 2: As a further improvement, parallel, or optional independent solution, the multi-purpose plug 6 is made entirely of rubber. The substantive technical effect and its implementation process, i.e., the basic function, of this technical solution are as follows: the rubber material provides flexible extrusion of beans, without damaging the beans themselves, resulting in better separation.

[0030] Example 3: As a further improvement, parallel, or optional independent solution, the sieve 15 includes a ring of arc-shaped side curved soft walls 16. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: the side curved soft walls 16 can guide the beans and water, but do not affect the movement of the overall side device, especially the overall movement of the multi-stage stopper linkage shaft 7.

[0031] Example 4: As a further improvement, parallel, or optional independent solution, a bottom container 11 is arranged under the sieve 15. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: The bottom container 11 is used to contain the mud-like material that falls and is squeezed through the sieve. This is the separated cells. The bottom container 11 is placed at the bottom during squeezing. Previously, when water was added, the container was not placed at the bottom.

[0032] Example 5: As a further improvement, parallel, or optional independent solution, the sieve fixing bracket 9 fixes multiple sieves 15 from top to bottom via the sieve fixing shaft 10, with the aperture of the multiple sieves 15 gradually decreasing from top to bottom; the multi-purpose plug 6 is connected to an additional plug at a position relative to the bottom via the plug linkage shaft 7, with each additional plug corresponding to one sieve 15. The substantial technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: multi-stage sieving achieves multi-stage extrusion.

[0033] Example 6: As a further improvement, parallel, or optional independent solution, the sieve fixing shaft 10 and the plug linkage shaft 7 are staggered. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: the staggered arrangement ensures that the two do not affect each other.

[0034] Example 7: As a further improvement, parallel, or optional independent solution, the additional plug is provided with an additional plug conical surface 18. The substantive technical effect and implementation process of the technical solution here, i.e., the basic function, is as follows: the additional plug conical surface 18 facilitates the automatic flow of bean paste.

[0035] Example 8: As a further improvement, parallel, or optional independent solution, the bottom container 11 can hold multiple material trays 21 in multiple layers. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: multiple material trays 21 can be directly transferred, enabling further freeze-drying, etc.

[0036] Example 9: As a further improvement, parallel, or optional independent solution, a temperature sensor and a level gauge are arranged in the multi-purpose container 8. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: controlling the temperature and level to achieve the soaking of beans.

[0037] Innovatively, each of the above effects exists independently, yet a single structure can be used to combine the results.

[0038] It should be noted that the multiple solutions provided in this patent include their own basic solutions, which are independent of each other and do not restrict each other. However, they can also be combined with each other without conflict to achieve multiple effects.

[0039] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims.

Claims

1. A flexible monomer soaking and separation device for separating cotyledon cells of mixed beans, characterized in that, The soaking and separating device includes an upper support (1), on which a water inlet (2) and a bean inlet (3) are arranged. The multipurpose container (8) is fixed to the two side walls of the upper bracket (1) by the multipurpose container fixing shaft (13); A lifting structure is arranged downward on the upper support (1), and a lifting shaft (4) extends from the lifting structure. The lifting shaft (4) is connected to a multi-purpose plug (6). The multi-purpose plug (6) has a conical surface (5) above it. The multi-purpose plug (6) can move up and down in the multi-purpose container (8). The bottom of the inner wall of the multi-purpose container (8) is provided with a side wall inner protrusion (14). When the multi-purpose plug (6) moves downward, it can be blocked by the side wall inner protrusion (14) to prevent it from falling further and thus limit its position. When the edge of the multi-purpose plug (6) fits the side wall inner protrusion (14), the bottom of the multi-purpose container (8) can be relatively closed. When the multi-purpose plug (6) moves upward appropriately, a gap (20) can appear. The upper support (1) is fixed with the sieve fixing bracket (9) facing downwards, and the sieve (15) is fixed in the middle of the sieve fixing bracket (9). A shaft is arranged downward on the upper support (1), and a heating device (12) is arranged at the end of the shaft. The heating device (12) is an electric heating device. The sieve (15) includes a ring of curved soft walls (16) on the side. The sieve fixing bracket (9) fixes multiple sieves (15) from top to bottom through the sieve fixing shaft (10), and the aperture of the multiple sieves (15) gradually decreases from top to bottom; the multi-purpose plug (6) is connected to an additional plug at the bottom position through the plug linkage shaft (7), and each additional plug corresponds to a sieve (15).

2. The flexible monomer soaking and separation device for separating cotyledon cells of mixed beans as described in claim 1, characterized in that, The multi-purpose plug (6) is made entirely of rubber.

3. The flexible monomer soaking and separation device for separating cotyledon cells of mixed beans as described in claim 1, characterized in that, A bottom container (11) is arranged under the sieve (15).

4. The flexible monomer soaking and separation device for separating cotyledon cells of mixed beans as described in claim 1, characterized in that, The fixed shaft (10) of the sieve and the linkage shaft (7) of the plug are arranged in a staggered manner.

5. The flexible monomer soaking and separation device for separating cotyledon cells of mixed beans as described in claim 1, characterized in that, Each additional plug is equipped with an additional plug conical surface (18).

6. The flexible monomer soaking and separation device for separating cotyledon cells of mixed beans as described in claim 3, characterized in that, The bottom container (11) can hold multiple material trays (21) with multiple layers.

7. The flexible monomer soaking and separation device for separating cotyledon cells of mixed beans as described in claim 1, characterized in that, A temperature sensor and a level gauge are arranged in the multipurpose container (8).

8. A method for separating flexible monomers from cotyledon cells of mixed beans by soaking, characterized in that, The soaking separation apparatus according to any one of claims 1-7 comprises the following steps: The same container enables soaking, water-bean separation, and crushing separation; Step 1: Soaking: When the edge of the multi-purpose plug (6) fits the inner convex ridge (14) of the side wall, the bottom of the multi-purpose container (8) can be relatively sealed; at this time, water is added to the water outlet (2) and peeled mixed beans are added to the bean outlet (3); then the heating device is started to soak in warm water; Step 2: Separating water and beans: When the lifting shaft (4) moves upward appropriately, a gap (20) appears, and the water beans fall from the gap and collect on the sieve (15); Step 3: Extrusion, crushing, and separation: Subsequently, the lifting structure moves back and forth, and the multi-purpose plug (6) gradually squeezes the mixed beans, causing the beans to break and break in multiple stages to achieve separation; during the multi-stage breaking, the additional plug cone surface (18) guides the flow of broken beans; at this time, water is added to the side for auxiliary rinsing.

Images