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Application of wogonin in preparing medicine for resisting herpes simplex virus

A technology of herpes simplex virus and wogonin, applied in antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of undisclosed anti-infection effects of wogonin, and achieve strong inhibition of herpes simplex virus Infectious activity, mitigation of HSV drug resistance, and low cytotoxicity

Inactive Publication Date: 2018-02-23
常州市武进人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this document does not disclose the anti-infective effect of wogonin on herpes simplex virus

Method used

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  • Application of wogonin in preparing medicine for resisting herpes simplex virus
  • Application of wogonin in preparing medicine for resisting herpes simplex virus
  • Application of wogonin in preparing medicine for resisting herpes simplex virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Wogonin inhibits the pathological effect of HEC-1-A cells caused by herpes simplex virus

[0030] HEC-1-A cells are derived from human endocervical adenocarcinoma. Previous studies have shown that they can support the infection and replication of HSV-1 and HSV-2 in their cells. Like Vero cells in HSV virology research, it is also a good cell model for studying the virus (Qiu M, et al. Pyrrolidine dithiocarbamate inhibits herpes simplex virus type 1 and 2 replication and its activity maybe mediated through dysregulation of ubiquitin-proteasome system. Journal of Virology , 2013, 87(15): 8675-8686.).

[0031] The HEC-1-A cells were divided into 4 groups, the first group was the blank control group, normal cultured HEC-1-A cells; the second group was the virus control group (HSV-2), added HSV-2 (G) HEC-1-A cells cultured in virus solution (moi=1); Group 3 is HEC-1-A cells incubated with Scutellaria baicalensis alone (Wog.), cultured with wogonin (final concentr...

Embodiment 2

[0034] Embodiment 2: Wogonin inhibits the replication test of herpes simplex virus on HEC-1-A cells

[0035] HEC-1-A cells were seeded on a 96-well plate at an appropriate density. After the cells were fused the next day, firstly, the cells were pre-incubated with gradiently diluted wogonin solutions of different concentrations, and then HSV-1 (HF) was added respectively. Or HSV-2 (G) (moi=1) to infect the cells. After culturing for 24 hrs, 200 μL of new medium was added to each well, and the cells in the 96-well plate were transferred to minus 80°C and 37°C for three times of freezing and thawing to release virus particles. The virus solution was diluted 5-fold, and 50 μL of the diluted solution was added to a 96-well plate with a monolayer of HEC-1-A cells, and the number of plaques was counted after 48 hr of incubation to calculate the virus titer.

[0036] The results of the plaque counting experiment were as follows: figure 2 It was shown that wogonin had inhibitory ef...

Embodiment 3

[0037] Example 3: Wogonin inhibits the replication test of herpes simplex disease at the RNA level

[0038] HEC-1-A cells were seeded in 24-well cell culture plates. After the cells were confluent, wogonin solutions of different concentrations were added in gradient dilutions, incubated at 37°C for 30 min, and then HSV-1 (HF) or HSV were added respectively. -2 (G), infection titer moi=1. After 24 hrs, discard the medium, add 200 μL Trizol to lyse the cells, and extract the total cellular RNA according to the steps described in the manual.

[0039] Using the TOYOBO reverse transcription kit instructions, the first strand of cDNA was synthesized and then qPCR was detected, and the HSV-1 gD gene (GenBank ID: 2703444), HSV-2 gD gene (GenBank ID: 1487358) and internal reference were detected by SYBR Green method Gene GAPDH amplification. The reaction system was 20 μL, and the reaction conditions were pre-denaturation at 95°C for 10 min; 95°C for 15 s, 60°C for 15 s, 72°C for 45 s,...

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Abstract

The invention belongs to the field of pharmacy, and relates to application of wogonin in preparing medicine for resisting a herpes simplex virus. An experiment verifies that wogonin has a high activity of resisting the herpes simplex virus in an in-vitro experiment and can inhibit the expression of virus structure protein gD in mRNA transcription and the protein level; an in-vitro toxicity experiment of wogonin finds that the flavone type compound has low toxicity to cells of the human and African green monkeys and can obtain a high treatment index; therefore, wogonin has potential value as novel medicine resisting the herpes simplex virus and can be applied to preparing the medicine for resisting the herpes simplex virus.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to the application of wogonin in the preparation of anti-herpes simplex virus medicine. Background technique [0002] Herpes simplex virus (HSV) is one of the most widely spread human pathogenic microorganisms, mainly divided into two types: herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2). The transmission of HSV is mainly dependent on contact, and its infection symptoms mainly include blisters on the skin or mucous membranes of the mouth, lips or genitals, which will form scabs when the skin lesions heal. HSV-1 is mainly transmitted through the oral route and establishes latent infection in the facial trigeminal ganglion of humans; while HSV-2 mainly relies on sexual transmission and establishes persistent latent infection in the sacral ganglion of humans. The HSV virus avoids the clearance of the immune system by hiding in the nervous system of the human body. When the immune functi...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P31/22
CPCA61K31/352
Inventor 楚鹰吴稚伟吕晓文张龙锋宋思维
Owner 常州市武进人民医院
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