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Method for evaluating quality of gnaphalium affine by quantitative-analysis-of-multi-components-by-single-marker (QAMS) method

A technology for quality evaluation and sagebrush, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of many varieties of standard products and complex content determination methods, so as to simplify the method, reduce the testing cost and testing time, and improve the efficiency. Effect

Active Publication Date: 2019-07-09
XIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a quality evaluation method of one test and multiple evaluations of S. sage, which solves the problem that there are many varieties of standard products and the content determination method of S. sage is complicated.

Method used

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  • Method for evaluating quality of gnaphalium affine by quantitative-analysis-of-multi-components-by-single-marker (QAMS) method
  • Method for evaluating quality of gnaphalium affine by quantitative-analysis-of-multi-components-by-single-marker (QAMS) method
  • Method for evaluating quality of gnaphalium affine by quantitative-analysis-of-multi-components-by-single-marker (QAMS) method

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Effect test

preparation example Construction

[0041] Preparation of the test solution: crush the sage grass, sieve through a 40-mesh sieve, accurately weigh the sieved powder of the sage grass, add methanol solution to constant volume, cold soak for 1h-10h, and filter through 0.22-0.45 μm microporous membrane, spare;

[0042]Take the sample solution prepared in step 2, accurately draw 10uL of each test solution, detect and analyze according to the HPLC chromatographic conditions in step 3, obtain a liquid chromatogram, perform peak positioning according to the relative retention time, and calculate each target compound in combination with the relative correction factor content. The results were compared with those calculated by the external standard method, and the content of each target compound was calculated. The present invention uses chlorogenic acid as an internal reference, calculates the relative correction factors of caffeic acid, isobracoside, and eupholzanthin, and investigates the reappearance of the relative...

Embodiment 1

[0046] Step 1, preparation of standard solution: take 5 mg of chlorogenic acid, isobracoside and eupholzanthin reference substance, and 10 mg of caffeic acid reference substance, weigh them accurately, place them in 10mL volumetric flasks, add methanol to dissolve, The volume was adjusted to the scale, and the mass concentration was prepared to be 0.52 mg / ml of chlorogenic acid, 1.06 mg / ml of caffeic acid, 0.53 mg / ml of isoverbacoside and 0.50 mg / ml of eupholzanthin. Accurately measure 1ml of the above-mentioned reference substance solution and place it in a 10ml volumetric flask, add methanol to the volume, and prepare the mass concentrations of chlorogenic acid 0.052mg / ml, caffeic acid 0.106mg / ml, isomacoside 0.053mg / ml and A mixed reference substance solution of 0.050mg / ml isoeuplavin;

[0047] Step 2, preparation of the test solution: Accurately weigh 0.1 g of the Herba skeletalum herb powder, put it in a 25 mL volumetric flask, add methanol to the volume, filter after col...

Embodiment 2

[0071] Step 1, preparation of standard solution: take 5 mg of chlorogenic acid, isobracoside and eupholzanthin reference substance, and 10 mg of caffeic acid reference substance, weigh them accurately, place them in 10mL volumetric flasks, add methanol to dissolve, The volume was adjusted to the scale, and the mass concentration was prepared to be 0.52 mg / ml of chlorogenic acid, 1.06 mg / ml of caffeic acid, 0.53 mg / ml of isoverbacoside and 0.50 mg / ml of eupholzanthin. Accurately measure 1ml of the above-mentioned reference substance solution and place it in a 10ml volumetric flask, add methanol to the volume, and prepare the mass concentrations of chlorogenic acid 0.052mg / ml, caffeic acid 0.106mg / ml, isomacoside 0.053mg / ml and A mixed reference solution of eupholzanthin 0.050mg / ml.

[0072] Step 2, preparation of the test solution: Accurately weigh 0.1g of the Herba sageria medicinal material powder, add 50mL of methanol, reflux for extraction for 1h, filter while hot and evapo...

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Abstract

The invention discloses a method for evaluating the quality of gnaphalium affine by a quantitative-analysis-of-multi-components-by-single-marker (QAMS) method. The method comprises the steps as follows: firstly, an HPLC content determination method for four main medicinal components in the gnaphalium affine is established; chlorogenic acid is used as an internal reference substance; relative correction factors of other three active components are calculated; the system applicability and the method reproducibility of the relative correction factors are inspected; chromatographic peak positioning is performed according to relative retention time; the content of each component to be determined is calculated in combination with the relative correction factors; and content determination resultsare proved to have no obvious difference by virtue of mutual verification of the QAMS method and an external standard method. The method disclosed by the invention solves the problems that the reference substance is high in cost and difficult to obtain; by virtue of the relative correction factors and the chromatographic peak positioning, the content of index components is calculated; synchronousdetermination of various medicinal substances in the gnaphalium affine is realized, so that the cost can be reduced, the operation is simplified, and the efficiency is improved; the method is high indetection sensitivity and high in stability; the determination results are accurate and reliable; and the method has a great significance on the quality control of the gnaphalium affine and the guarantee of the clinical effects.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and relates to a quality evaluation method of one-test and multiple-evaluation of sagebrush. Background technique [0002] Gnaphlium affine (Gnaphlium affine D.Don), also known as: sage ear grass, velvet ear grass, chasing wind bone, Qingming vegetable, autumn chrysanthemum grass, etc., belongs to the whole herb of Gnaphlium affine D. Don, a plant of Compositae. Used as a medicine, it is sweet and flat in nature and taste, non-toxic, and has the effects of eliminating phlegm, relieving cough, relieving asthma, and dispelling rheumatism; especially the effect of moistening the lungs and resolving phlegm, it can replace Fritillaria sichuan as medicine, and its medicinal material standards are recorded in "Chinese Pharmacopoeia" (1977 ) edition, "Jiangsu Provincial Standards of Chinese Medicinal Materials" (1989) edition. Studies have shown that Shamrock...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张寒张彦郑梦迪汪兴军申旭霁张艳茹
Owner XIAN MEDICAL UNIV
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