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A kind of quality evaluation method of one test and multiple evaluations of sage

A quality evaluation, sagebrush technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex content determination methods and many varieties of standard products, reduce detection costs and detection time, simplify methods, and simplify quantification The effect of the detection method

Active Publication Date: 2022-04-22
XIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a quality evaluation method of one test and multiple evaluations of S. sage, which solves the problem that there are many varieties of standard products and the content determination method of S. sage is complicated.

Method used

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  • A kind of quality evaluation method of one test and multiple evaluations of sage
  • A kind of quality evaluation method of one test and multiple evaluations of sage
  • A kind of quality evaluation method of one test and multiple evaluations of sage

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preparation example Construction

[0041] Preparation of the test solution: crush the sage grass, sieve through a 40-mesh sieve, accurately weigh the sieved powder of the sage grass, add methanol solution to constant volume, cold soak for 1h-10h, and filter through 0.22-0.45 μm microporous membrane, spare;

[0042]Take the sample solution prepared in step 2, accurately draw 10uL of each test solution, detect and analyze according to the HPLC chromatographic conditions in step 3, obtain a liquid chromatogram, perform peak positioning according to the relative retention time, and calculate each target compound in combination with the relative correction factor content. The results were compared with those calculated by the external standard method, and the content of each target compound was calculated. The present invention uses chlorogenic acid as an internal reference, calculates the relative correction factors of caffeic acid, isobracoside, and eupholzanthin, and investigates the reappearance of the relative...

Embodiment 1

[0046] Step 1, preparation of standard solution: take 5 mg of chlorogenic acid, isobracoside and eupholzanthin reference substance, and 10 mg of caffeic acid reference substance, weigh them accurately, place them in 10mL volumetric flasks, add methanol to dissolve, The volume was adjusted to the scale, and the mass concentration was prepared to be 0.52 mg / ml of chlorogenic acid, 1.06 mg / ml of caffeic acid, 0.53 mg / ml of isoverbacoside and 0.50 mg / ml of eupholzanthin. Accurately measure 1ml of the above-mentioned reference substance solution and place it in a 10ml volumetric flask, add methanol to the volume, and prepare the mass concentrations of chlorogenic acid 0.052mg / ml, caffeic acid 0.106mg / ml, isomacoside 0.053mg / ml and A mixed reference substance solution of 0.050mg / ml isoeuplavin;

[0047] Step 2, preparation of the test solution: Accurately weigh 0.1 g of the Herba skeletalum herb powder, put it in a 25 mL volumetric flask, add methanol to the volume, filter after col...

Embodiment 2

[0071] Step 1, preparation of standard solution: take 5 mg of chlorogenic acid, isobracoside and eupholzanthin reference substance, and 10 mg of caffeic acid reference substance, weigh them accurately, place them in 10mL volumetric flasks, add methanol to dissolve, The volume was adjusted to the scale, and the mass concentration was prepared to be 0.52 mg / ml of chlorogenic acid, 1.06 mg / ml of caffeic acid, 0.53 mg / ml of isoverbacoside and 0.50 mg / ml of eupholzanthin. Accurately measure 1ml of the above-mentioned reference substance solution and place it in a 10ml volumetric flask, add methanol to the volume, and prepare the mass concentrations of chlorogenic acid 0.052mg / ml, caffeic acid 0.106mg / ml, isomacoside 0.053mg / ml and A mixed reference solution of eupholzanthin 0.050mg / ml.

[0072] Step 2, preparation of the test solution: Accurately weigh 0.1g of the Herba sageria medicinal material powder, add 50mL of methanol, reflux for extraction for 1h, filter while hot and evapo...

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Abstract

The invention discloses a method for evaluating the quality control of sagebrush by one-test and multiple-evaluation method. Firstly, the HPLC content determination method of four main medicinal components in sagebrush is established, and chlorogenic acid is used as an internal reference to calculate the other three kinds of activities. The relative correction factors of the components were investigated, and the system applicability and method reproducibility of the relative correction factors were investigated. The color peaks were positioned according to the relative retention time, and then combined with the relative correction factors to calculate the content of each component to be tested, through the one-test and multiple-evaluation method The mutual verification with the external standard method proves that there is no significant difference in the content determination results. The method of the present invention overcomes the problems of high cost and difficult availability of reference substances, calculates the content of index components through relative correction factors and chromatographic peak positioning, and realizes the simultaneous determination of multiple medicinal substances in Shamrock, which can save costs, simplify operations, and improve Efficiency, high detection sensitivity, good stability, accurate and reliable measurement results, which are of great significance to the quality control and clinical efficacy of sagebrush.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and relates to a quality evaluation method of one-test and multiple-evaluation of sagebrush. Background technique [0002] Gnaphlium affine (Gnaphlium affine D.Don), also known as: sage ear grass, velvet ear grass, chasing wind bone, Qingming vegetable, autumn chrysanthemum grass, etc., belongs to the whole herb of Gnaphlium affine D. Don, a plant of Compositae. Used as a medicine, it is sweet and flat in nature and taste, non-toxic, and has the effects of eliminating phlegm, relieving cough, relieving asthma, and dispelling rheumatism; especially the effect of moistening the lungs and resolving phlegm, it can replace Fritillaria sichuan as medicine, and its medicinal material standards are recorded in "Chinese Pharmacopoeia" (1977 ) edition, "Jiangsu Provincial Standards of Chinese Medicinal Materials" (1989) edition. Studies have shown that Shamrock...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张寒张彦郑梦迪汪兴军申旭霁张艳茹
Owner XIAN MEDICAL UNIV
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