Method for establishing fingerprint spectrum of lung-ventilating and toxin-vanquishing prescription

A technology of fingerprinting and establishing methods, which is applied in the field of formula component detection, can solve problems such as inability to fully control quality, complex components of traditional Chinese medicine compound, and difficulty in showing the overall efficacy of traditional Chinese medicine compound with a single active ingredient, so as to achieve the effect of ensuring quality and curative effect

Active Publication Date: 2021-06-04
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0003] Traditional Chinese medicine compound is composed of a variety of traditional Chinese medicines, and has the principle of holistic view and syndrome differentiation. Due to the complexity of the composition of th

Method used

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  • Method for establishing fingerprint spectrum of lung-ventilating and toxin-vanquishing prescription
  • Method for establishing fingerprint spectrum of lung-ventilating and toxin-vanquishing prescription
  • Method for establishing fingerprint spectrum of lung-ventilating and toxin-vanquishing prescription

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the drawings and embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments.

[0025] The instrument equipment that adopts in this implementation example is Waters Acquity UHPLC ultra-high performance liquid chromatograph, U.S. Waters company; MSA225P-0CE-DU type 1 / 100,000 balance, Germany Sartorius company; BP121S type 1 / 10,000 balance, Germany Sartorius company; 5430R high-speed refrigerated centrifuge, Eppendorf, Germany; Milli-Qacademic ultrapure water system, Millipore, USA.

[0026] In this implementation example, the selected materials are reference substance ephedrine hydrochloride (batch number: 171241-201809), polydatin (batch number: 111575-200502) purchased from China Institute for Drug and Food Control; reference substance amygdalin (batch number: DST190829-004) , verbenin (lot number: DST19101...

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Abstract

The invention discloses a method for establishing a fingerprint spectrum of a lung-ventilating and toxin-vanquishing prescription. The method comprises the following steps of: calculating RSD values of relative retention time and relative peak areaof each common peak by taking naringin as a standard substance; according to the invention, the fingerprint spectrum of the lung-ventilating and toxin-vanquishing prescription is established through the UHPLC method, and the contents of the nine effective components are determined, so that reference is provided for research on pharmacodynamic material basis and quality control of the lung-ventilating and toxin-vanquishing prescription.

Description

technical field [0001] The invention belongs to the technical field of formula component detection, in particular to a method for establishing the fingerprint of Xuanfei Baidu prescription. Background technique [0002] The new crown pneumonia belongs to the category of plague with damp poison as the disease, which can be called damp poison plague. Xuanfei Baidu Decoction is a traditional Chinese medicine formula proposed for clinical manifestations and syndrome differentiation, which is used in the treatment of patients with new coronary pneumonia with common dampness and stagnation of lung syndrome in the seventh edition of the diagnosis and treatment plan. The prescription is derived from the addition and subtraction of several classic famous prescriptions, including Maxing Shigan Decoction, Maxing Yigan Decoction, Tingli Dazao Xiefei Decoction, Qianjin Weigan Decoction, etc., and is made by combining 13 traditional Chinese medicines. [0003] Traditional Chinese medicin...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686
Inventor 常艳旭张晗陈炫好李晋何俊高秀梅张伯礼
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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