Method for carrying out quality control on platycodon grandiflorum (traditional Chinese herb) based on bioactivity assay

Abstract

The invention relates to a method for carrying out quality control on platycodon grandiflorum (a traditional Chinese herb) based on bioactivity assay, which effectively realizes the quality control on platycodon grandiflorum and ensures the safe and effective clinical application of platycodon grandiflorum. The method comprises the following steps: crushing platycodon grandiflorum, and carrying out ultrasonic extraction on the crushed platycodon grandiflorum by using ethanol so as to obtain a dry matter; carrying out multiple proportion diluting on the obtained dry matter into seven groups by using normal saline according to an initial concentration at an agent distance of 0.5, thereby obtaining samples; preparing the platycodon grandiflorum into working reference substances according to the sample steps; dissolving phenol red by using a sodium bicarbonate solution, then making a standard curve through taking the dose of the phenol red as a x-coordinate and taking an OD (optical density) value as a y-coordinate; taking animals as experimental objects, grouping the animals, carrying out drug administration respectively, and putting the animals subjected to drug administration to death; then taking tracheas of the animals and putting the tracheas into normal saline, centrifuging and then taking supernate, determining the OD value at a wavelength of 546 nm, and calculating and comparing the secreted phenol-red content of the tracheas according to the phenol-red standard curve; and with the phenol-red content as an index, carrying out potency calculation. The method disclosed by the invention is easy to operate, stable, reliable, accurate in test and good in quality control effect, and can provide new ideas and references for the quality control and quality evaluation of other traditional Chinese herbs with undefined pharmaceutical active substances.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for quality control of traditional Chinese medicine Platycodon grandiflorum based on biological activity measurement. Background technique [0002] The current internal quality control of traditional Chinese medicine still mainly adopts the method of qualitative detection and / or content determination of some index components. However, the chemical components contained in traditional Chinese medicine are complex, and most of the effective substances of traditional Chinese medicine are not very clear. The consistency and stability of the quality of traditional Chinese medicines are difficult to correlate with or reflect the safety and effectiveness of their clinical use. The "biological activity determination" of traditional Chinese medicine has become one of the key development methods of traditional Chinese medicine quality control. The "Guiding Principles for the Determination ...

AI Technical Summary

Problems solved by technology

[0003] Platycodon grandiflorum, a traditional Chinese medicine, is one of the most commonly used expectorant drugs in clinical practice. The current quality standard is mainly to determine the single index platycodon grandiflora saponin D (C 57 h 92 o 28 ), and the chemical background of Platycodon grandiflora is relatively complicated. According to reports, more than 39 kinds of saponins have been isolated from Platycodon grandiflorum alone, and a considerable part of them have significant antitussive and expectorant effects. In addition, flavonoids, phenols, and volatile oils Seedlings, fatty oils, fatty acids, etc. are also its active ingredients, so the quality control index of Platycodon grandiflorum D alone has little correlation with drug efficacy, and it cannot fully achieve the internal quality control target of Platycodon grandiflorum. Therefore, It is imperative to develop a new internal quality control method for Platycodon grandiflorum

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