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Fingerprint spectrum construction method and application of Chinese herbal compound containing angelica sinensis

A technology for traditional Chinese medicine and compound prescription, which is applied in the field of fingerprint spectrum construction of traditional Chinese medicine compound prescription, can solve the problem that traditional Chinese medicine has no mandatory requirements, and achieves the effects of good peak shape, good separation and good repeatability.

Active Publication Date: 2021-07-23
HUNAN YINENG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the State Drug Administration has required relevant fingerprints for traditional Chinese medicine injections, which has played a key role in improving the quality of traditional Chinese medicine injections, but there is no mandatory requirement for other dosage forms of traditional Chinese medicine

Method used

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  • Fingerprint spectrum construction method and application of Chinese herbal compound containing angelica sinensis
  • Fingerprint spectrum construction method and application of Chinese herbal compound containing angelica sinensis
  • Fingerprint spectrum construction method and application of Chinese herbal compound containing angelica sinensis

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Effect test

preparation example Construction

[0086] Preparation of the test solution: add water or alcohol to the Chinese medicine compound to obtain the Chinese medicine compound solution; and filter the Chinese medicine compound solution to obtain the test solution after ultrasonic extraction or reflux extraction, wherein the Chinese medicine compound Angelica, Guizhi, Licorice, Paeoniae Alba, Ginger and Jujube;

[0087] Preparation of reference solution: weigh gallic acid, paeoniflorin, ferulic acid, liquiritin, glycyrrhizic acid amine and 6-gingerol, add 30%-70% methanol to make the concentration of each component is 0.02~0.2mg· mL -1 The reference substance solution;

[0088] The chromatographic conditions for high-performance liquid phase detection are: adopt C 18 Chromatographic column, using methanol or acetonitrile as mobile phase A, acid aqueous solution as mobile phase B, gradient elution program: 0-20min, 5%-12%A; 20-90min, 12%-85%A; flow rate 0.8 ~1.2mL / min; column temperature is 25~40℃; detection wavelen...

Embodiment

[0116] The preparation method of need testing solution

[0117] Take 12g angelica, 9g cassia twig, 6g roasted licorice, 18g white peony, 9g ginger and 12g jujube, add 2000ml of water, soak for 50 minutes, decoct for 150 minutes, filter with a 120-mesh double-layer filter cloth, the filtrate is set aside, and freeze-dried , to obtain about 19 g of freeze-dried powder. Take about 0.9g of the above freeze-dried powder, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 25ml of water, weigh it, ultrasonically treat it for 15 minutes (power 250W40kHz), take it out, let it cool, weigh it, and make up with water Subtract the lost weight, shake well, filter, and take the continued filtrate to obtain the test solution.

[0118] The preparation method of reference substance solution

[0119] Take the appropriate amount of ammonium glycyrrhizinate reference substance, ferulic acid reference substance, gallic acid reference substance, liquiritin reference substa...

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Abstract

The invention discloses a detection method of a Chinese herbal compound containing angelica sinensis. The method comprises the following steps: taking a Chinese herbal compound test sample and reference substances for detection, wherein the Chinese herbal compound comprises angelica sinensis, cassia twig, licorice, white peony root, ginger and jujube; the reference substances are gallic acid, paeoniflorin, ferulic acid, liquiritin, ammonium glycyrrhizinate and 6-gingerol; and the chromatographic conditions of the detection are as follows: a C18 chromatographic column is adopted, methanol or acetonitrile is used as a mobile phase A, an acid aqueous solution is used as a mobile phase B, and a gradient elution procedure includes that 5%-12% A for 0-20 min is performed; 12%-85% A for 20-90 min is performed; a flow velocity is 0.8-1.2 mL / min; a column temperature is 25-40 DEG C; and a detection wavelength is 210-330 nm; and obtaining component information or component and content information of the Chinese herbal compound according to a detection result. According to the invention, the chemical components of the Chinese herbal compound containing the angelica sinensis are comprehensively and systematically analyzed by applying a high performance liquid chromatography technology, and a theoretical basis is provided for deep research on quality control and a pharmacodynamic material basis.

Description

technical field [0001] The application relates to the technical field of quality identification of traditional Chinese medicines, in particular to a method for constructing fingerprints of Chinese medicine compound containing angelica and its application. Background technique [0002] With the rapid development of traditional Chinese medicine preparations in my country, the quality management of traditional Chinese medicine preparations has also received widespread attention, and its quality will directly affect the quality of medical care and the safety of patients. Factors affecting the quality of traditional Chinese medicine preparations mainly include: (1) the quality of the original medicinal materials. The medicine market often obtains a lot of economic benefits from shoddy products. In addition, the supply of wild medicinal materials in my country's medicinal materials market is relatively lacking, coupled with the country's restrictions on the collection of wild nat...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8686G01N30/8679G01N2030/027
Inventor 易跃能朱亚文魏丹丹陈锋林俊华
Owner HUNAN YINENG BIOLOGICAL PHARMA
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