Method for evaluating chemical composition of Rosa xanthina on basis of antithrombotic spectrum-effect relationship
A technology of chemical composition and evaluation methods, applied in scientific instruments, material separation, analysis of materials, etc.
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[0043] The first step, preparation of the test solution: take an appropriate amount of Rosa japonica and properly pulverize it, accurately weigh 6 parts of coarse powder, each 100g, place it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol, reflux extraction for 1 hour, extract twice, filter, combine the filtrate, the filtrate is recovered solvent, and vacuum dried to obtain a dry extract; weigh an appropriate amount of recovered solvent dry sample, dissolve in water Then go to macroporous resin chromatography column, elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, recover the solvent, and dry in vacuum to obtain a dry extract;
[0044] The second step, the establishment of the fingerprints of rose thorns: adopt high performance liquid chromatography to measure the fingerprints of the samples obtained in step one; chromatographic conditions: the chromatographic column adopts oc...
Embodiment 1
[0070] 1 Experimental method
[0071] 1.1 Establishment of fingerprints
[0072] 1.1.1 Preparation of the test product
[0073] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 hour, extraction twice, filtration, combined filtrates, solvent recovery and vacuum drying of filtrates to obtain dry extracts, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.
[0074] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of ...
Embodiment 2
[0117] 1 Experimental method
[0118] 1.1 Establishment of fingerprints
[0119] 1.1.1 Sample preparation
[0120] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 h, extraction twice, filtration, combined filtrate, filtrate recovered solvent, vacuum drying to obtain dry extract, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.
[0121] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of each sample from S6 to S...
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