Method for evaluating chemical composition of Rosa xanthina on basis of antithrombotic spectrum-effect relationship

Abstract

The invention discloses a method for evaluating chemical composition of Rosa xanthina on the basis of antithrombotic spectrum-effect relationship. The method comprises the following steps: preparing extract of different polar components of the Rosa xanthina with a modern separation technology; establishing fingerprint of extract of each component with high-performance liquid chromatography, and calibrating characteristic peaks; evaluating antithrombotic activity of different extract on the basis of platelet aggregation inhibition rate, prothrombin time, thrombin time and activated partial thromboplastin time as indexes; substituting fingerprint characteristic peak data and pharmacodynamical activity data into a mathematical model for spectrum-effect correlation analysis, and evaluating pharmacodynamical activity of the characteristic peaks. With adoption of the method for evaluating the chemical composition of the Rosa xanthina on the basis of the antithrombotic spectrum-effect relationship, the antithrombotic chemical composition in the Rosa xanthina can be evaluated rapidly and accurately, a scientific and effective method is provided for research of pharmacodynamic material basis and quality control of the Rosa xanthina, and reference is provided for further development of Rosa xanthina drugs or health care products for treating thrombotic diseases.

Description

technical field

[0001] The invention relates to a method for evaluating the efficacy and activity of chemical components of traditional Chinese medicines, in particular to a method for evaluating the chemical components of Rosa japonica based on the antithrombotic spectrum-effect relationship. Background technique

[0002] Yellow thorn rose (Rosa xanthina Lindl.), also known as yellow thornberry, is a perennial deciduous shrub, and is the fruit of wild thorn rose of the genus Rosa in the family Rosaceae. Widely distributed in mountainous and hilly areas in Northeast, North China, Northwest and other regions. According to the "Dictionary of Chinese Medicine", wild thorn rose fruit has the effects of invigorating the spleen and regulating qi, nourishing blood and regulating menstruation, and can treat indigestion, qi stagnation, diarrhea, stomach pain, irregular menstruation and other diseases. Folks eat a lot of food for tea, wine and so on. Modern research shows that rose ...

Examples

preparation example Construction

[0043] The first step, preparation of the test solution: take an appropriate amount of Rosa japonica and properly pulverize it, accurately weigh 6 parts of coarse powder, each 100g, place it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol, reflux extraction for 1 hour, extract twice, filter, combine the filtrate, the filtrate is recovered solvent, and vacuum dried to obtain a dry extract; weigh an appropriate amount of recovered solvent dry sample, dissolve in water Then go to macroporous resin chromatography column, elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, recover the solvent, and dry in vacuum to obtain a dry extract;

[0044] The second step, the establishment of the fingerprints of rose thorns: adopt high performance liquid chromatography to measure the fingerprints of the samples obtained in step one; chromatographic conditions: the chromatographic column adopts oc...

Embodiment 1

[0070] 1 Experimental method

[0071] 1.1 Establishment of fingerprints

[0072] 1.1.1 Preparation of the test product

[0073] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 hour, extraction twice, filtration, combined filtrates, solvent recovery and vacuum drying of filtrates to obtain dry extracts, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.

[0074] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of ...

Embodiment 2

[0117] 1 Experimental method

[0118] 1.1 Establishment of fingerprints

[0119] 1.1.1 Sample preparation

[0120] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 h, extraction twice, filtration, combined filtrate, filtrate recovered solvent, vacuum drying to obtain dry extract, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.

[0121] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of each sample from S6 to S...

technical field

[0001] The invention relates to a method for evaluating the efficacy and activity of chemical components of traditional Chinese medicines, in particular to a method for evaluating the chemical components of Rosa japonica based on the antithrombotic spectrum-effect relationship. Background technique

[0002] Yellow thorn rose (Rosa xanthina Lindl.), also known as yellow thornberry, is a perennial deciduous shrub, and is the fruit of wild thorn rose of the genus Rosa in the family Rosaceae. Widely distributed in mountainous and hilly areas in Northeast, North China, Northwest and other regions. According to the "Dictionary of Chinese Medicine", wild thorn rose fruit has the effects of invigorating the spleen and regulating qi, nourishing blood and regulating menstruation, and can treat indigestion, qi stagnation, diarrhea, stomach pain, irregular menstruation and other diseases. Folks eat a lot of food for tea, wine and so on. Modern research shows that rose ...

Examples

preparation example Construction

[0043] The first step, preparation of the test solution: take an appropriate amount of Rosa japonica and properly pulverize it, accurately weigh 6 parts of coarse powder, each 100g, place it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol, reflux extraction for 1 hour, extract twice, filter, combine the filtrate, the filtrate is recovered solvent, and vacuum dried to obtain a dry extract; weigh an appropriate amount of recovered solvent dry sample, dissolve in water Then go to macroporous resin chromatography column, elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, recover the solvent, and dry in vacuum to obtain a dry extract;

[0044] The second step, the establishment of the fingerprints of rose thorns: adopt high performance liquid chromatography to measure the fingerprints of the samples obtained in step one; chromatographic conditions: the chromatographic column adopts oc...

Embodiment 1

[0070] 1 Experimental method

[0071] 1.1 Establishment of fingerprints

[0072] 1.1.1 Preparation of the test product

[0073] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 hour, extraction twice, filtration, combined filtrates, solvent recovery and vacuum drying of filtrates to obtain dry extracts, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.

[0074] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of ...

Embodiment 2

[0117] 1 Experimental method

[0118] 1.1 Establishment of fingerprints

[0119] 1.1.1 Sample preparation

[0120] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 h, extraction twice, filtration, combined filtrate, filtrate recovered solvent, vacuum drying to obtain dry extract, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.

[0121] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of each sample from S6 to S...

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