Evaluation method for chemical constituents of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectrum-effect

A technology of chemical composition and evaluation method, applied in the direction of scientific instruments, material separation, analysis of materials, etc., to achieve the effects of clear characteristic peaks, strong objectivity and strong reproducibility

Active Publication Date: 2022-01-25
GUANGXI UNIV OF CHINESE MEDICINE
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  • Evaluation method for chemical constituents of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectrum-effect
  • Evaluation method for chemical constituents of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectrum-effect
  • Evaluation method for chemical constituents of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectrum-effect

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Embodiment Construction

[0054] The present invention will be further described below in conjunction with specific examples, but the protection scope and application scope of the present invention will not be limited.

[0055] 1. Instruments and materials

[0056] 1. Instrument

[0057] Agilent1100 High Performance Liquid Chromatography (Agilent, USA), UV-1780 UV-Vis Spectrophotometer (Shimadzu Instrument (Suzhou) Co., Ltd.), INFINITE 200PRO Microplate Reader (Austria TECAN Company), SorvallST16R High-speed Refrigerated Centrifuge (Thermo Fisher Technology Company, USA), KQ-500DA ultrasonic instrument (Kunshan Ultrasonic Instrument Co., Ltd.), RF-6000 fluorescence spectrophotometer (Shimadzu Corporation, Japan).

[0058] 2. Materials

[0059] Longan leaf samples: Collected in Wuzhou City, Guangxi, identified as Dimocarpus longan Lour. by Professor Teng Jianbei of Guangxi University of Traditional Chinese Medicine.

[0060] Reference substance: ascarin (Shanghai Ronghe Pharmaceutical Technology Co.,...

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Abstract

The invention discloses a method for evaluating the chemical components of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectral effects. The method establishes HPLC fingerprints of different polar parts of longan leaves, calibrates the common characteristic peaks, and evaluates the chemical components through the mathematical analysis model of spectral effects. Antioxidative and hypoglycemic effects of chemical constituents in longan leaves. The method of the present invention has the advantages of convenience and conciseness, strong objectivity, strong reproducibility, and clear characteristic peaks, and can quickly and accurately evaluate the chemical components in longan leaves that have anti-oxidation and hypoglycemic effects, which is the basis for the medicinal effect of longan leaves Research and quality control provide a scientific and effective method, and provide a reference for the further development of longan leaves for anti-oxidation and hypoglycemic effects of drugs or health food.

Description

technical field [0001] The invention relates to a method for evaluating the efficacy and activity of chemical components of traditional Chinese medicine, in particular to a method for evaluating the chemical components of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectrum-effect. Background technique [0002] Longan leaf is a traditional Chinese medicinal material with Guangxi national characteristics, which was first recorded in "Southern Yunnan Bencao Tushuo". For the famous subtropical fruit - longan (Dimocarpus longan Lour.) tree leaves or shoots. Its nature and flavor are sweet, light, and flat, and it has the functions of clearing heat, dampness and detoxification. It is mainly used to treat cold, fever, malaria, boils, eczema, etc. Longan originates in the subtropical regions of southern my country and northern Vietnam, and has been cultivated in my country for more than 2,000 years. my country's longan cultivation area and out...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 梁洁麦嘉妮赵立春林婧黄光强陈晓思
Owner GUANGXI UNIV OF CHINESE MEDICINE
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