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Oxidation resistance and blood sugar level spectrum-efficiency relationship-based longan leaf chemical composition evaluating method

A technology of chemical composition and evaluation method, applied in the direction of scientific instruments, material separation, analysis of materials, etc., to achieve the effects of clear characteristic peaks, strong reproducibility, and scientific and perfect quality control

Active Publication Date: 2019-06-07
GUANGXI UNIV OF CHINESE MEDICINE
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  • Oxidation resistance and blood sugar level spectrum-efficiency relationship-based longan leaf chemical composition evaluating method
  • Oxidation resistance and blood sugar level spectrum-efficiency relationship-based longan leaf chemical composition evaluating method
  • Oxidation resistance and blood sugar level spectrum-efficiency relationship-based longan leaf chemical composition evaluating method

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Embodiment Construction

[0054] The present invention will be further described below in conjunction with specific examples, but the protection scope and application scope of the present invention will not be limited.

[0055] 1. Instruments and materials

[0056] 1. Instrument

[0057] Agilent1100 High Performance Liquid Chromatography (Agilent, USA), UV-1780 UV-Vis Spectrophotometer (Shimadzu Instrument (Suzhou) Co., Ltd.), INFINITE 200PRO Microplate Reader (Austria TECAN Company), SorvallST16R High-speed Refrigerated Centrifuge (Thermo Fisher Technology Company, USA), KQ-500DA ultrasonic instrument (Kunshan Ultrasonic Instrument Co., Ltd.), RF-6000 fluorescence spectrophotometer (Shimadzu Corporation, Japan).

[0058] 2. Materials

[0059] Longan leaf samples: Collected in Wuzhou City, Guangxi, identified as Dimocarpus longan Lour. by Professor Teng Jianbei of Guangxi University of Traditional Chinese Medicine.

[0060] Reference substance: ascarin (Shanghai Ronghe Pharmaceutical Technology Co.,...

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Abstract

The invention discloses an oxidation resistance and blood sugar level decrease spectrum-efficiency relationship-based longan leaf chemical composition evaluating method. According to the oxidation resistance and blood sugar level spectrum-efficiency relationship-based longan leaf chemical composition evaluating method, the HPLC fingerprint spectrum of the different polar parts of a longan leaf isestablished, and common characteristic peaks are calibrated; and the oxidation resistance and blood sugar level decrease effects of chemical compositions in the longan leaf are evaluated through a spectrum-efficiency mathematical analysis model. The method has the advantages of convenience, simplicity, high objectivity, high reproducibility and clear characteristic peaks. With the method adopted,the chemical compositions in the longan leaf which have the oxidation resistance and blood sugar level decrease effects can be quickly and accurately evaluated, a scientific and effective method can be provided for longan leaf pharmacodynamic material basis research and quality control, and reference can be provided for the further development of longan leaf medicines or health care food which have oxidation resistance and blood sugar level decrease effects.

Description

technical field [0001] The invention relates to a method for evaluating the efficacy and activity of chemical components of traditional Chinese medicine, in particular to a method for evaluating the chemical components of longan leaves based on the relationship between anti-oxidation and hypoglycemic spectrum-effect. Background technique [0002] Longan leaf is a traditional Chinese medicinal material with Guangxi national characteristics, which was first recorded in "Southern Yunnan Bencao Tushuo". For the famous subtropical fruit - longan (Dimocarpus longan Lour.) tree leaves or shoots. Its nature and flavor are sweet, light, and flat, and it has the functions of clearing heat, dampness and detoxification. It is mainly used to treat cold, fever, malaria, boils, eczema, etc. Longan originates in the subtropical regions of southern my country and northern Vietnam, and has been cultivated in my country for more than 2,000 years. my country's longan cultivation area and out...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 梁洁麦嘉妮赵立春林婧黄光强陈晓思
Owner GUANGXI UNIV OF CHINESE MEDICINE
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