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An evaluation method for chemical components of Rosa japonica based on antithrombotic spectrum-effect relationship

A technology of chemical composition and evaluation method, applied in scientific instruments, material separation, analysis of materials, etc.

Active Publication Date: 2021-04-20
山西省医药与生命科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the existing problems, the object of the present invention is to provide a method for evaluating the chemical components of Rosa japonica based on the antithrombotic spectrum-effect relationship, which can quickly and accurately evaluate the chemical components and active ingredients with antithrombotic effect in Rosa japonica Screening provides a scientific and effective method for the basic research and quality control of the medicinal substances of Rosa japonica, so as to solve the problems raised in the above-mentioned background technology

Method used

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  • An evaluation method for chemical components of Rosa japonica based on antithrombotic spectrum-effect relationship
  • An evaluation method for chemical components of Rosa japonica based on antithrombotic spectrum-effect relationship
  • An evaluation method for chemical components of Rosa japonica based on antithrombotic spectrum-effect relationship

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] The first step, preparation of the test solution: take an appropriate amount of Rosa japonica and properly pulverize it, accurately weigh 6 parts of coarse powder, each 100g, place it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol, reflux extraction for 1 hour, extract twice, filter, combine the filtrate, the filtrate is recovered solvent, and vacuum dried to obtain a dry extract; weigh an appropriate amount of recovered solvent dry sample, dissolve in water Then go to macroporous resin chromatography column, elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, recover the solvent, and dry in vacuum to obtain a dry extract;

[0044] The second step, the establishment of the fingerprints of rose thorns: adopt high performance liquid chromatography to measure the fingerprints of the samples obtained in step one; chromatographic conditions: the chromatographic column adopts oc...

Embodiment 1

[0070] 1 Experimental method

[0071] 1.1 Establishment of fingerprints

[0072] 1.1.1 Preparation of the test product

[0073] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 hour, extraction twice, filtration, combined filtrates, solvent recovery and vacuum drying of filtrates to obtain dry extracts, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.

[0074] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of ...

Embodiment 2

[0117] 1 Experimental method

[0118] 1.1 Establishment of fingerprints

[0119] 1.1.1 Sample preparation

[0120] Take an appropriate amount of Rosa japonica and crush it properly, accurately weigh 6 parts of coarse powder, each 100g, put it in a round bottom flask, add 10 times the volume of water, 30% ethanol, 50% ethanol, 70% ethanol and 90% ethanol respectively Ethanol, reflux extraction for 1 h, extraction twice, filtration, combined filtrate, filtrate recovered solvent, vacuum drying to obtain dry extract, samples were recorded as S1-S5, and set aside. Weigh an appropriate amount of sample S4, dissolve it in water, put it on a macroporous resin chromatography column, and elute with 40%, 60%, and 80% ethanol solution respectively, collect each eluted part, concentrate under reduced pressure to a dry extract, and set aside, sample number It is S6~S8.

[0121] Take 1g of each sample from S1 to S5 (equivalent to 10g of raw material), take 0.1g of each sample from S6 to S...

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Abstract

The invention discloses a method for evaluating chemical components of Rosa japonica based on antithrombotic spectrum-effect relationship. Extracts of different polar components of Rosa japonica are prepared by using modern separation technology; The fingerprints of the extracts, and the characteristic peaks were calibrated; the antithrombotic activity of different extracts was evaluated with the platelet aggregation inhibition rate, prothrombin time, thrombin time and partial thrombin time as indicators; the characteristic peak data of the fingerprints and the pharmacodynamic activity The data is brought into the mathematical model for spectrum-effect correlation analysis to evaluate the pharmacodynamic activity of the characteristic peaks. This evaluation method for the chemical components of Rosa japonica based on the antithrombotic spectrum-effect relationship can quickly and accurately evaluate the chemical components in Rosa japonica with antithrombotic effect, and provide scientific and effective information for the basic research and quality control of Rosa japonica. The method provides a reference for the further development of medicines or health care products for the treatment of thrombotic diseases.

Description

technical field [0001] The invention relates to a method for evaluating the efficacy and activity of chemical components of traditional Chinese medicines, in particular to a method for evaluating the chemical components of Rosa japonica based on the antithrombotic spectrum-effect relationship. Background technique [0002] Yellow thorn rose (Rosa xanthina Lindl.), also known as yellow thornberry, is a perennial deciduous shrub, and is the fruit of wild thorn rose of the genus Rosa in the family Rosaceae. Widely distributed in mountainous and hilly areas in Northeast, North China, Northwest and other regions. According to the "Dictionary of Chinese Medicine", wild thorn rose fruit has the effects of invigorating the spleen and regulating qi, nourishing blood and regulating menstruation, and can treat indigestion, qi stagnation, diarrhea, stomach pain, irregular menstruation and other diseases. Folks eat a lot of food for tea, wine and so on. Modern research shows that rose ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 杨莹莹张璐王进东阮文辉
Owner 山西省医药与生命科学研究院
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