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Use of a safflower extract with clear spectrum-effect relationship

A safflower extract and extract technology, applied in the field of safflower extract, can solve problems such as inability to effectively express correlation, no curative effect, instability and the like

Active Publication Date: 2019-07-23
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The fingerprints of traditional Chinese medicine can better reflect the characteristics of "multi-component, multi-target, and multi-channel comprehensive regulation" of traditional Chinese medicine. However, the active ingredients and their material basis of most traditional Chinese medicine are not clear at present. The fingerprints of traditional Chinese medicines above the ingredients cannot effectively express their correlation with the drug efficacy. Therefore, only the fingerprints marked with the characteristic peaks of the active ingredients of traditional Chinese medicines are matched with the results of their medicinal effects, that is, the content of chemical components in the fingerprints of traditional Chinese medicines. Only by linking the change with its efficacy change and establishing the spectrum-effect relationship of traditional Chinese medicine can it effectively reflect its internal quality, which is the key to the research of traditional Chinese medicine.
[0008] During the in-depth research, it was found that the complex extraction process, especially the process with many extraction steps and complicated processes, can easily change the fingerprint characteristics of the extract. Although the content of the known components in the extract meets the requirements, the activity is often There are serious defects, resulting in a decline in curative effect, instability, or even no curative effect
[0009] In all the published invention patents after searching, there is no research on the spectrum-effect relationship of safflower extract, and no effective control of the fingerprint characteristic spectrum of the extract

Method used

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  • Use of a safflower extract with clear spectrum-effect relationship
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  • Use of a safflower extract with clear spectrum-effect relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The safflower samples from different sources were collected from the main producing areas of Xinjiang safflower: Changji Jimsar County, Tacheng Yumin County, Yining Huocheng County, a total of 10 batches.

[0033] Weigh 15g of the safflower medicinal material and pulverize it, add 40 times the amount of distilled water, soak in a water bath at a temperature of 70°C for 30 minutes, reflux extraction for 3 times, each time for 40 minutes, and combine the extracts;

[0034] Concentrating the extract under reduced pressure to a relative density of 1.01-1.05g / mL to obtain a concentrate;

[0035]Load the concentrated solution onto the AB-8 macroporous adsorption resin column. The ratio of the diameter to the height of the resin chromatography column is 1:9, and the elution rate is 1mL / min. When the reaction is basically negative, elute with 10% ethanol at a volume concentration, collect the fractions, concentrate the extract to obtain 628 mg of yellow powder, dry and store at...

Embodiment 2

[0037] Weigh 15g of the safflower medicinal material and pulverize it, add 20 times the amount of distilled water, soak in a water bath at a temperature of 60°C for 120 minutes, and extract once under reflux for 100 minutes to obtain an extract;

[0038] Concentrating the extract to a relative density of 1.01-1.05g / mL to obtain a concentrate;

[0039] Load the concentrated solution to the AB-8 macroporous adsorption resin column. The ratio of the diameter to the height of the resin chromatography column is 1:9, and the elution rate is 1mL / min. When the reaction is basically negative, elute with volume concentration 10% ethanol, collect the fractions, concentrate the extract to obtain 556 mg of yellow powder, dry and store at room temperature to obtain the safflower extract.

Embodiment 3

[0041] The biological activity screening of the safflower extract that embodiment 1 obtains as the medicine of protein tyrosine phosphatase inhibitor or the purposes of health product:

[0042] Screening method: Use disodium p-nitrophenyl phosphate (pNPP) as a substrate, and determine the activity of PTP1B inhibitors according to the color reaction produced by protein tyrosine phosphatase 1B (PTP1B) hydrolyzing the phosphate group of pNPP. PTP1B inhibitory activity assay reaction system: 179 μL of PTP1B dilution, diluted with phosphate buffered saline solution PBS of pH 7.4, incubated at room temperature for 5 min, 1 μL of test sample or positive control sample (make the sample into a concentration gradient) or dimethyl sulfoxide (DMSO ) 1 μL, mix well and incubate at room temperature for 5 minutes, add 20 μL of 35 mmol / L pNPP, the total volume is 200 μL, incubate at 25 °C in the dark for 30 min, then stop the reaction, measure the absorbance at 405 nm, and use the enzyme-free ...

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Abstract

The invention relates to applications of safflower extract with the definite spectrum-effect relationship. An hypoglycemic extract is obtained from safflower carthamus through distilled water reflux extraction and macroporous resin separation and purification, and the medical applications of the hypoglycemic extract are illustrated preliminarily; the sum of the peak areas of hydroxysafflor yellow A and 6-hydroxyl kaempferol-3,6-bis-O-glucose-7-O-glucoside in an HPLC (High Performance Liquid Chromatography) fingerprint of the extract is not less than 50%; through calculation, the peak area ratio of hydroxysafflor yellow A to 6-hydroxyl kaempferol-3,6-bis-O-glucose-7-O-glucoside is 5.0-8.0. The activity screening test proves that the extract obtained by adopting the method provided by the invention has the obvious effect of inhibiting protein-tyrosine-phosphatase 1B (PTP-1B), has the excellent effect of reducing blood glucose on SD (Sprague Dawley) rats suffering from diabetes induced by high-fat diet and streptozotocin (STZ), and can be taken as the raw material or adjuvant to be used for preparing medicines or health products for preventing and treating diabetes.

Description

technical field [0001] The invention relates to the use of a safflower extract with a clear spectrum-effect relationship. Background technique [0002] Safflower is the dried flower of safflower (Carthamus tinctorius L.), which has the effects of promoting blood circulation, promoting menstruation, dispelling blood stasis and relieving pain. Used for amenorrhea, dysmenorrhea, lochia, mass in the abdomen, chest pain and heartache, abdominal pain due to stasis, stabbing pain in the chest and hypochondrium, injury from falls, swelling and pain in girth ulcer. Since Zhang Qian introduced safflower from the Western Regions to my country, it has a history of more than 2100 years of cultivation and medicinal use. Safflower is mainly produced in Xinjiang, and the Uyghur medicine is called Zhalangziguli, which is one of the traditional medicinal materials for promoting blood circulation and removing blood stasis. Through years of research on safflower, it has been determined that i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/286A23L33/00A61P3/10A61K133/00
CPCA23V2002/00A61K36/286A23V2200/328
Inventor 信学雷武鹤婷赛那瓦尔·芒思尔阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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