Pharmacodynamic material basis screening method of artemisia capillaries soup

A kind of material basis, the technology of Artemisia capriculum, applied in the field of medicine, can solve the problems of long time period, high cost and large consumption of screening active ingredients

Active Publication Date: 2014-10-15
王喜军
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of long time period, large consumption and high cost of screening active ingredients in the existing Artemisia chinensis Decoction, and to provide a method for screening Artemisia chinensis. The method of material basis of decoction efficacy

Method used

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  • Pharmacodynamic material basis screening method of artemisia capillaries soup
  • Pharmacodynamic material basis screening method of artemisia capillaries soup
  • Pharmacodynamic material basis screening method of artemisia capillaries soup

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specific Embodiment approach 1

[0039] Embodiment 1: In this embodiment, the method for screening the effective substance basis of Yinchenhao Decoction is carried out according to the following steps:

[0040] 1. Establishment of peak areas of components in Yinchenhao Decoction:

[0041] (1) Preparation of the test sample: Weigh 18g of Artemisia chinensis, add 1400mL of water to boil, keep boiling and decoct to 700mL, add 9g gardenia and 6g rhubarb, keep boiling and decoct for 10min, filter the decoction with gauze, and concentrate the filtrate to The concentration of Artemisia annua is 1g / mL, and it is made into freeze-dried powder for later use;

[0042] (2) Preparation of samples for in vivo analysis: Add water to the freeze-dried powder prepared in step (1) to make a solution with a concentration of 0.65 g / mL, and give Wistar male rats intragastric administration at a dose of 1 mL / 100 g, and adopt the hepatic portal vein blood collection method Blood was taken 15 minutes after the administration, and th...

specific Embodiment approach 2

[0069] Embodiment 2: The difference between this embodiment and Embodiment 1 is that both the urine and blood samples are centrifuged at 4° C. and 10,000 rpm for 5 min as described in Step 2. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0070] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the high-performance liquid chromatography condition described in step two is: mobile phase is B phase 0.1% formic acid acetonitrile and A phase mass percent content is 0.1 % formic acid solution, the flow rate is 0.50mL / min. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a pharmacodynamic material basis screening method of artemisia capillaries soup, relates to a pharmacodynamic material basis screening method of a traditional Chinese medicine, and solves the problems of long time cycle, large consumption and high cost of screening of effective components of the artemisia capillaries soup in the prior art. The pharmacodynamic material basis screening method is as follows: 1, establishment of peak area of body components of the artemisia capillaries soup; 2, establishment of metabonomics biological markers of the artemisia capillaries soup on the basis of CCL4 liver injury model; and 3, establishment of plotting of correlation between marker metabolites and serum constituents (PCMS) research method. The invention provides the pharmacodynamic material basis screening method of the traditional Chinese medicine, the pharmacodynamic material basis screening method is fast, simple, accurate, low-cost, small in consumption, and better in versatility, can effectively determine the efficacy-related components, can reflect the efficacy index comprehensive effect characteristic of multi components, and can be used for pharmacodynamic material basis screening.

Description

technical field [0001] The invention relates to a method for screening the effective substance basis of traditional Chinese medicine, and relates to the field of medicine. Background technique [0002] Traditional Chinese medicine is an important material basis for the clinical prevention and treatment of diseases in traditional Chinese medicine. It has the characteristics of multi-component, multi-channel, multi-target, and multi-effect overall regulation, which makes the research on the material basis, biological effects and their relationship of Chinese medicine compound quite complicated. Excavating and interpreting the active ingredients of traditional Chinese medicine is the basis for the development of safe, effective and quality-controllable modern Chinese medicine, and it is the prerequisite for revealing the scientific connotation of Chinese medicine and guiding the rational use of drugs in clinical practice. Due to the complexity of the traditional Chinese medicin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 王喜军张爱华
Owner 王喜军
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