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Hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings

A technology of Dendrobium officinale and sterile seedlings is applied in the field of bioengineering, which can solve the problems of high cultivation cost, use of hormones and the like, and achieve the effects of simple operation steps, low production cost and improved medicinal value.

Inactive Publication Date: 2009-10-28
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is easy to operate, low in cost, and does not use hormones in the culture process, which better solves the problems of high culture cost and extensive use of hormones in the tissue culture process of Dendrobium officinale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The capsules of Dendrobium officinale collected from Nanning, Guangxi, after surface disinfection, obtain aseptic seeds, and the seeds are sown on the surface of the aseptic germination solid medium. The medium consists of: KC medium, adding sucrose 20g / L, banana 150g / L, After boiling for 20 minutes, take the juice and add it to the medium, the agar is 9.0g / L, pH5.2. The culture bottle is placed under the condition that the temperature is 20-25° C., and the light is blue light for protocorms and seedling induction conditions.

[0034] After cultivating under the above conditions for 5 months, the seeds germinated to form seedlings, and some of the sown triangular flasks were taken out and transferred to root-inducing conditions for cultivation. The cultivation conditions were: the light was produced by fluorescent lamps, and the light was 10 hours a day, and the light intensity was 1500Lx , the culture temperature is 25±2°C, and cultured for 4 months to induce the forma...

Embodiment 2

[0041] The capsules of Dendrobium officinale collected from Nanning, Guangxi, after surface disinfection, to obtain aseptic seeds, the seeds were sown in containers filled with sterile germination solid medium, the composition of the medium was: MS medium with halved macronutrients, added sucrose 30g / L, inositol 100mg / L, coconut milk 100g / L, pH5.8, agar 9.0g / L. The culture conditions are: the temperature is 15-25°C, and the light is yellow light.

[0042] After cultivating under the above conditions for 10 months, the seeds germinated to form seedlings with 3 leaves, and some of the culture containers were taken out and transferred to root-inducing conditions for cultivation. The culture conditions were: natural light, 8-10 hours of light per day, light intensity It is 2000-2500Lx, the culture temperature is 20-25°C, and it is cultivated for 3 months to induce the formation of rooted sterile seedlings.

[0043]Transfer the induced aseptic rooted seedlings to the strong seedli...

Embodiment 3

[0045] The capsules of Dendrobium candidum grown after artificial pollination collected from Chishui, Guizhou, were sterile seeds were obtained after surface disinfection, and the seeds were sown in containers filled with sterile germination solid medium. The medium consisted of: B5 medium, added 50g / L sucrose, 9.0g / L agar, pH 5.8; culture conditions: temperature 15-25°C, culture without light. After culturing for 2 months, the seeds germinate to form protocorms, and then the culture container is transferred to a green light environment to continue culturing at a temperature of 20-25°C.

[0046] After 5 months of cultivation, take out part of the culture container and transfer it to the condition of inducing rooting: natural light, 8-10 hours of light per day, light intensity of 2000-2500Lx, culture temperature of 20-25°C, culture for 4 months to induce Rooted sterile seedlings are formed.

[0047] Subculture the above-mentioned rooted sterile seedlings into strong seedling m...

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Abstract

The invention belongs to the biotechnology field and relates to a hormone-free cultivation and rapid propagation method of dendrobium candidum axenic seedlings. The hormone-free cultivation and rapid propagation method comprises the steps: germinating dendrobium candidum seeds in an axenic mode; forming and preserving protocorms and seedlings; rooting and proliferating unrooted seedlings, cultivating sound seedlings; and toughening and transplanting the axenic seedlings. The technology has convenient operation, small occupied space and low production cost, is convenient to industrially produce and apply on a large scale and is beneficial to retaining the excellent property of the seedlings and increase the use safety of the seedlings when being used for propagating the dendrobium candidum.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for hormone-free cultivation and rapid propagation of dendrobium officinale sterile seedlings. technical background [0002] Dendrobium is a traditional traditional Chinese medicine with good curative effect. According to the records of "Chinese Pharmacopoeia" 2005 edition, Dendrobium has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat, relieving throat and improving eyesight, and regulating human immunity. There are more than 30 species of Dendrobium that can be used as medicine, among which Dendrobium candidum Wall.ex Lindl is the most valuable. panda. Due to the increase in usage and the shortage of resources, its price has been soaring, and the current price of dry products has reached more than 20,000 yuan per kilogram. Driven by profit, the wild Dendrobium candidum resources are destructivel...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/02A01G31/00C12N5/04
Inventor 郭顺星房慧勇侯晓强陈晓梅邢晓科李燕
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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