Method for obtaining germination effective symbiotic fungus for Cymbidium bicolor seeds

Abstract

The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.

Description

technical field [0001] The present invention relates to the method for obtaining the effective symbiotic fungus of Orchidaceae seed germination, specifically relates to a kind of litter, bark, moss and humus etc. which are collected in the original place of Cymbidium bicolor as culture substrate. A method for inducing seed germination by chamber culture, obtaining protocorms, and then isolating, cultivating and screening effective symbiotic fungi for seed germination from the protocorms. Background technique [0002] The seeds of orchids are very small, with only immature embryos. Under natural conditions, seed germination requires specific symbiotic fungi to obtain nutrients. At present, the germination of Orchidaceae seeds mostly uses artificial medium for non-symbiotic germination under sterile conditions. Although the germination rate is high, when the endangered species are returned to the wild, the survival rate of the seedlings transplanted to the natural environment ...

AI Technical Summary

Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fungi with unknown functions in the roots of Orchidaceae, this makes the screening and isolation of effective fungi for seed germination complicated and cumbersome. , meanwhile, whether the fungi in the roots of different orchids are the same as the effective symbiotic fungi at the seed germination stage is still uncertain