An officinal dendrobium stem plantation method
A cultivation method and technology of Dendrobium candidum, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve the problems of slow growth of litholysis, low survival rate, low yield rate, poor air permeability, etc., and achieve survival High rate, satisfying epiphytic characteristics, and good air permeability
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Embodiment 1
[0011] Embodiment 1: Dendrobium candidum seeds are put into the culture medium of 1 / 2MS basic medium+NAA (a-naphthalene acetic acid) 0.4mg / L+10% banana juice+0.7% agar through conventional disinfection procedures, and the seed Germination culture, culture temperature 22~25℃, transfer the formed protocorm into 1 / 2MS basic medium + BA (6-benzyl adenine) 0.2mg / L + 10% banana juice + 0.7% agar medium , carry out protocorm proliferation culture, and then transfer the grown seedlings to the medium of 1 / 2MS basic medium + NAA0.4mg / L + 10% banana juice + 0.7% agar, and carry out rooting and cultivation of strong seedlings into medium seedlings, about 2 months; then transfer the middle seedlings to the same culture medium for large seedling cultivation. In about 2 months, large seedlings with a height of more than 5 cm can be cultivated, and thick bottle seedlings with a seedling height of more than 3 cm can be cultivated in bottles. The seedling substrate soil used for seedling harden...
Embodiment 2
[0013] Embodiment 2: Dendrobium candidum seeds are put into the medium of 1 / 2MS basic medium+NAA (a-naphthaleneacetic acid) 0.4mg / L+10% banana juice+0.7% agar through conventional disinfection procedures, and the seeds are Germination culture, culture temperature 22~25℃, transfer the formed protocorm into 1 / 2MS basic medium + BA (6-benzyl adenine) 0.2mg / L + 10% banana juice + 0.7% agar medium , carry out protocorm proliferation culture, and then transfer the grown seedlings to the medium of 1 / 2MS basic medium + NAA0.4mg / L + 10% banana juice + 0.7% agar, and carry out rooting and cultivation of strong seedlings into medium seedlings, about 2 months; then transfer the middle seedlings to the same culture medium for large seedling cultivation. In about 2 months, large seedlings with a height of more than 5 cm can be cultivated, and thick bottle seedlings with a seedling height of more than 3 cm can be cultivated in bottles. The hardening matrix soil used for hardening seedlings i...
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