Breeding technology of konjak testtube taro
A konjac technology, applied in the field of agricultural biology, can solve problems such as difficult management, high technical requirements, and difficult to hold, and achieve the effect of high survival rate and reproduction rate and simple management
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[0009] First wash the tuber-shaped konjac explants, and take the terminal bud part according to the specification of 0.5cm×0.5cm, and then soak them in 75% alcohol for 2-3 seconds and 0.1% mercury liter for 15 minutes, and then use aseptic Rinse with water, put the konjac tuber explants into MS+6BA after washing 6~0.1 mg / L+NAA 6~0 mg / L, PH5.8 medium, cultivated in an environment with a temperature above 20°C and a light of 1000LX for 45 days to form seedlings at one time. At this time, the tissue-cultured seedlings were put into the same medium for subculture, once every 20 days, and the multiplication ratio was 1:4-1:6. During the subculture process, the temperature is above 20°C and the light is 1000LX. When the seedlings grow to a height of 3-4cm, cut the ramets and put them into the rooting medium MS+NAA with 1-6ppm of paclobutrazol 5~1 The rooting culture in mg / L medium is 20-25 days, and the tubers expand while the tissue culture seedlings take root, forming small bul...
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