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Breeding technology of konjak testtube taro

A konjac technology, applied in the field of agricultural biology, can solve problems such as difficult management, high technical requirements, and difficult to hold, and achieve the effect of high survival rate and reproduction rate and simple management

Inactive Publication Date: 2004-05-05
云南省农业科学院生物技术与种质资源研究所
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of difficult management, high technical requirements, and difficult to be supported when transplanting konjac tissue culture seedlings, and provide a konjac test-tube taro propagation technology with simple management and high survival rate

Method used

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Embodiment Construction

[0009] First wash the tuber-shaped konjac explants, and take the terminal bud part according to the specification of 0.5cm×0.5cm, and then soak them in 75% alcohol for 2-3 seconds and 0.1% mercury liter for 15 minutes, and then use aseptic Rinse with water, put the konjac tuber explants into MS+6BA after washing 6~0.1 mg / L+NAA 6~0 mg / L, PH5.8 medium, cultivated in an environment with a temperature above 20°C and a light of 1000LX for 45 days to form seedlings at one time. At this time, the tissue-cultured seedlings were put into the same medium for subculture, once every 20 days, and the multiplication ratio was 1:4-1:6. During the subculture process, the temperature is above 20°C and the light is 1000LX. When the seedlings grow to a height of 3-4cm, cut the ramets and put them into the rooting medium MS+NAA with 1-6ppm of paclobutrazol 5~1 The rooting culture in mg / L medium is 20-25 days, and the tubers expand while the tissue culture seedlings take root, forming small bul...

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Abstract

A test tube technique for reproducing the konjak features that the paclobutrazol is added to the culture medium MS+NAA5-1 mg / L used for rooting the tissue culture seedlings of konjak, they are cultured at temp higher than 20 deg.C, under light radiation (1000 LX) for 20-25 days to form small bulb, when the weight of small bulb reeches 2-4 g they are cultured at lower than 15 deg.C in dark environment, after cultured in flask for 10-15 day they are sprouted and then transplanted in seedbed, and they are cultured at higher than 20 deg.C, while the fertilizer is proportionally and periodically applied.

Description

Technical field: [0001] The invention relates to a technique for multiplying konjac test-tube taro, which belongs to the field of agricultural biotechnology. Background technique: [0002] Konjac is the only plant that can synthesize a large amount of glucomannan. Glucomannan has a wide range of functions in industry, agriculture, medicine, and health food. It has attracted more and more people's interest and attention, and the planting area has continued to expand. However, the high disease rate of taro species and the serious degradation of excellent varieties restrict the development of this industry. Propagation of virus-free high-quality seedlings and taros through biotechnology has been successful in various plants such as potatoes and lilies, and has been widely used in production, achieving good social and economic benefits. At present, konjac has been able to breed excellent seedlings through biotechnology and transplant them to seedbeds for breeding, which provide...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王玲马继琼房亚南李勇军
Owner 云南省农业科学院生物技术与种质资源研究所
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