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Breeding technology of konjak testtube taro

A konjac and test tube technology, applied in the field of agricultural biology, can solve the problems of difficult management, high technical requirements, and difficult to hold, and achieve the effects of high survival rate and reproduction rate, and simple management.

Inactive Publication Date: 2005-06-01
云南省农业科学院生物技术与种质资源研究所
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of difficult management, high technical requirements, and difficult to be supported when transplanting konjac tissue culture seedlings, and provide a breeding technology of konjac test tube taro with simple management and high survival rate

Method used

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Embodiment Construction

[0009] First wash the tuber-shaped konjac explants, and take the terminal bud part according to the specification of 0.5cm×0.5cm, and then soak them in 75% alcohol for 2-3 seconds and 0.1% mercury liter for 15 minutes, and then use aseptic Rinse with water, put the washed konjac tuber explants into MS+6BA6-0.1mg / L+NAA6-0mg / L, PH5.8 medium, and cultivate them in an environment with a temperature above 20°C and a light of 1000LX for 45 days Seedlings can be grown once. At this time, the tissue-cultured seedlings were put into the same medium for subculture, once every 20 days, and the multiplication ratio was 1:4-1:6. During the subculture process, the temperature is above 20°C and the light is 1000LX. When the seedlings grow to a height of 3-4cm, cut them by division, put them into the rooting medium MS+NAA5-1mg / L with 1-6ppm of paclobutrazol, and cultivate them for 20-25 days. Expand to form small bulbs. When the bulblet grows to 2-4 grams, dark culture is carried out, the ...

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Abstract

The invention relates to a konjac test-tube taro breeding technology, which belongs to the field of agricultural biotechnology. The present invention consists of conventional procedures, wherein: when konjac tissue culture seedlings are rooted, 1-6ppm of paclobutrazol is added to the rooting medium MS+NAA5-1mg / L, and cultivated in an environment with a temperature above 20°C and a light of 1000LX for 20- On the 25th day, the tubers expanded while the tissue culture seedlings took root, forming small bulbs. When the bulblet grows to 2-4 grams, dark culture is carried out, the culture temperature is below 15°C, and the tissue culture seedlings are poured in the bottle for 10-15 days to form mature bulblets. After the mature small bulbs are out of the bottle, they are implanted in the seedbed after germination treatment, and nutrient is applied in a period of 30 days under the condition of proper shade above 20°C. The ratio of nutrient application is N:P:K:Ca:Mg=5 :1:5:0.5:1. This breeding technique just can harvest 100-200 grams of seed taro and 4-5 young taro in the same year. It has the advantages of high survival rate and reproduction rate, simple management and so on.

Description

Technical field: [0001] The invention relates to a konjac test-tube taro breeding technology, which belongs to the field of agricultural biotechnology. Background technique: [0002] Konjac is the only plant that can synthesize a large amount of glucomannan. Glucomannan has a wide range of functions in industry, agriculture, medicine, and health food. It has attracted more and more people's interest and attention, and the planting area has continued to expand. However, the high disease rate of taro species and the serious degradation of excellent varieties restrict the development of this industry. Propagation of virus-free high-quality seedlings and taros through biotechnology has been successful in various plants such as potatoes and lilies, and has been widely used in production, achieving good social and economic benefits. At present, konjac has been able to breed excellent seedlings through biotechnology and transplant them to seedbeds for breeding, which provides an e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王玲马继琼房亚南李勇军
Owner 云南省农业科学院生物技术与种质资源研究所
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