Cyrtopterin tissue culturing method and fast reproduction thereof

A technique of tissue culture and rhododendron orchid, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of rhododendron orchid reproduction difficulties, lack of seedlings, etc., to solve the shortage of seedlings, short production cycle, simplify The effect of the training program

Inactive Publication Date: 2006-08-16
BIO TECH INST GUIZHOU AGRI ACAD
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for rapid propagation of rhododendron orchid tissue culture, to solve the prob...

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  • Cyrtopterin tissue culturing method and fast reproduction thereof

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Embodiment 1

[0016] Embodiment 1 adopts Rhododendron orchid capsule seeds as explants. On the ultra-clean workbench, first wipe the capsule surface with 70% alcohol cotton balls for disinfection, then dip the capsules in alcohol and burn them for sterilization; Cut open the capsule with a scalpel, spread the tiny seeds evenly on the surface of 1 / 2MS solid medium supplemented with 0.5mg / L NAA, the pH of the medium is 5.5-5.8, and place it at a temperature of 25±2°C in complete darkness After half a year of cultivation, the seeds germinate and form small white protocorms. Transfer this small protocorm to B with 3.0~6.0mg / L 6-BA+0.2mg / L NAA+0.1% activated carbon+2% sugar 5 Solid proliferation medium, pH 5.5-5.8, placed at a temperature of 25±2°C, light time 12h / d, and light intensity 1500-2000lx, the small white protocorms will gradually turn green and form clustered protocorms with a long surface. It is covered with white hairs, and some stem nodes are elongated to form rhizomes. After 40-...

Embodiment 2

[0017] Embodiment 2 adopts the bud eye on the rhododendron orchid pseudobulb as the explant, rinses the soil on it with clear water first, dries, then packs into a triangular flask, and pours it into a triangular flask with 70% alcohol under aseptic conditions. Sterilize the pseudobulbs in a bottle for 20 seconds, then sterilize them by immersing them in 0.1% mercuric acid solution for 8 minutes, and then wash them with sterile water for 5 times, each time for 3 minutes; finally dry the water with sterile filter paper, cut out the bud eyes on the pseudocorms, and inoculate them. In the pre-prepared MS medium, the pH value of the medium is 5.5-5.8, placed at a temperature of 25±2°C, a light time of 12h / d, and a light intensity of 1500-2000lx, about 10d will start to germinate and form white small protocorms. After 30 days, the protocorm was transferred to the proliferation medium for culture, and a protocorm in cluster shape was obtained. The surface of the corm was covered with...

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Abstract

A tissue culture and fast reproduction method of azalea orchid includes such steps as aseptic sowing and culturing of azalea seeds to grow protocorm, or disinfecting and inoculating the bud eye of pseudocorm to obtain protocorm, culturing in the culture medium for reproducing by 3-7 times, differentiating to become seedlings, promoting their rooting to obtain regenerated plants, transplanting in vermiculite bed for transition or directly in vermiculite for rooting and germinating, transplanting in soil and later-phase management.

Description

technical field [0001] The invention relates to a method for plant regeneration through tissue culture technology, in particular to a method for tissue culture and rapid propagation of rhododendron orchids. Background technique [0002] Rhododendron orchid (Cremastra appendiculata Makino), also known as Mao Sigu, Abacus seven, etc., is a medicinal plant of the genus Rhododendron orchid in the family Orchidaceae. Cheng) and used as medicine, its taste is sweet and slightly pungent, cold in nature, slightly poisonous, has the effect of reducing swelling and reducing phlegm, detoxifying and resolving stagnation. Rhododendron orchid is not only a rare medicinal plant, but also a third-level rare and endangered wild protected plant in my country. The growth of Rhododendron orchid has strict requirements on the ecological environment. Its seeds are very small and have no endosperm. The success rate of seed reproduction is extremely low; the reproduction coefficient of rhododendro...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/00C12N5/04
Inventor 毛堂芬刘作易朱国胜黄永会
Owner BIO TECH INST GUIZHOU AGRI ACAD
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