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A method for efficiently obtaining cluster buds and rooted seedlings at the same time using the whip buds of the beautiful bamboo as explants

A technology of explant and bamboo whip, applied in the field of plant tissue culture, can solve problems such as unreported, and achieve the effect of overcoming low efficiency

Active Publication Date: 2022-04-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the ways of bud propagation of bamboo adopt the method of stalk segment, but there is no report on the propagation of buds by using bamboo pendant buds as explants

Method used

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  • A method for efficiently obtaining cluster buds and rooted seedlings at the same time using the whip buds of the beautiful bamboo as explants
  • A method for efficiently obtaining cluster buds and rooted seedlings at the same time using the whip buds of the beautiful bamboo as explants
  • A method for efficiently obtaining cluster buds and rooted seedlings at the same time using the whip buds of the beautiful bamboo as explants

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Embodiment 1

[0029] A method for simultaneously efficiently obtaining cluster buds and rooted seedlings using the whip buds of beautiful bamboo as explants, comprising the following steps:

[0030] (1) Selection of explants: as figure 1 As shown, select the side buds of the strong plants in the two-year-old container seedlings of Liangzhu as the explant material; cut the whip section of the container seedling into 1-1.5cm section with buds, wash it with detergent, and put it under running water. Rinse for 3-4h, then soak in Tween 80 for 10min, and rinse under running water for 0.5-1h.

[0031] (2) Establishment of a sterile system: the explants were disinfected and sterilized by 70% alcohol for 45 seconds, washed with sterile water for 3-5 times, 5% sodium hypochlorite for 15 minutes, and washed with sterile water for 3-5 times. After inoculation of the bamboo whip buds treated by the above disinfection methods, the sterility rate was the highest, and the survival rate of bud induction wa...

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Abstract

The invention discloses a method for simultaneously efficiently obtaining cluster buds and rooted seedlings by taking the whip buds of beautiful bamboo as explants, and belongs to the technical field of plant tissue culture. In this method, the lateral buds of strong plants growing in two-year-old beautiful bamboo container seedlings are used as explants, and the best sterilization time is selected after being treated with different disinfection times; finally, the sterile explants are inoculated on the induction medium Induce cluster buds; then through the multiplication medium, a large number of beautiful bamboo cluster buds and rooted seedlings can be induced at the same time. The method of the present invention fills up the blank of the rapid propagation of the clumps of the beautiful bamboo tissue culture, the clumps of the clumps of the beautiful bamboos with the whip buds as the explants grow faster and the number of buds tilled out is larger, and the rooted seedlings are obtained while the clumps proliferate, The rapid multiplication time of the beautiful bamboo is shortened, and the tissue culture efficiency of the beautiful bamboo is greatly improved. It has laid a foundation for rapid and sufficient supply for the garden planting and large-scale greening of beautiful bamboo, and also provided a feasible solution for the large-scale factory production of beautiful bamboo.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for efficiently obtaining cluster buds and rooted seedlings at the same time by taking the whip buds of the beautiful bamboo as explants. Background technique [0002] Beautiful bamboo (Sasaella glabra f.albostriata Muroi), a species of Sasaella glabra f.albostriata Muroi in North America, is one of the excellent ornamental bamboo species with colorful leaves, introduced from Japan, and is a mixed bamboo (Geng Bosuke, 1996) . Bamboo plants are short, 50-80cm high, 2-2.5mm thick, 12cm long internodes, green leaves with yellow-white vertical stripes, dense between plants, strong tillering ability, beautiful colors, often recognized as ornamental bamboo species. With the improvement of people's living standard and cultural level, the ornamental value and aesthetic value of bamboo are also gradually improved, and its importance is becoming more and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 林树燕王星杨蒙郑毅丁雨龙张春霞
Owner NANJING FORESTRY UNIV
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