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Method for obtaining large number of bambusa glaucophylla regeneration plants through tissue culture

A technology for plant regeneration and tissue culture, applied in the field of plant tissue culture, can solve problems such as difficult regeneration, achieve the effects of solving reproductive difficulties and overcoming the low efficiency of mother plant transplantation

Active Publication Date: 2015-06-17
扬州大禹风景竹园
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Problems solved by technology

[0004] Purpose of the invention: In view of the deficiencies in existing bamboo plant propagation techniques, the purpose of the present invention is to provide a method for obtaining a large number of bamboo regeneration plants through tissue culture, to overcome the difficult problem of regeneration in the tissue culture process of bamboo, and to provide The cultivation of bamboo regenerated plants provides a convenient, fast and effective method

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  • Method for obtaining large number of bambusa glaucophylla regeneration plants through tissue culture
  • Method for obtaining large number of bambusa glaucophylla regeneration plants through tissue culture
  • Method for obtaining large number of bambusa glaucophylla regeneration plants through tissue culture

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Embodiment 1

[0024] A method for obtaining a large amount of bamboo regeneration plants by tissue culture, comprising the following steps:

[0025] (1) Selection of explants: such as figure 1 As shown, the semi-lignified branches with full shoots and buds of Xiuzhu in the same year were selected as explant materials; the explants collected from the outdoors were first washed with running water for 2-3 hours, then treated with 70% ethanol for 45 seconds, and then treated with 0.1% HgCl 2 After soaking for 8 minutes, the explants of Xiuzhu explants treated by the above disinfection methods were inoculated, the sterility rate was the highest, and the survival rate of bud induction was high (Table 1).

[0026] Table 1 Effects of different disinfectants on explant culture for different time

[0027] deal with 70% ethanol(s) 0.1%HgCl 2 (min) Pollution rate (%) Sterility rate (%) Survival rate (%) 1 30 3 28.7 71.3 62.3 2 30 5 17.2 82.8 68.2 3 30 8 6...

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Abstract

The invention discloses a method for obtaining a large number of bambusa glaucophylla regeneration plants through tissue culture. The method comprises the following steps: disinfecting plump half-lignified branches of annual branch buds of bambusa glaucophylla serving as explants to form induced buds on an improved induction culture medium; inoculating the cluster buds onto an improved proliferation culture medium to induce the production of a large number of cluster buds; differentiating roots on a rooting medium to form complete plants; transplanting the complete plants into flower pots to complete the tissue culture process of bambusa glaucophylla. By adopting the method, the blank of tissue culture and rapid propagation of bambusa glaucophylla is filled up, the rapid and sufficient basis is laid for garden planting and large-area greening of the bambusa glaucophylla, and a feasible scheme is provided for the large-scale factory production of the bambusa glaucophylla.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining a large amount of regenerated plants of Phyllostachys japonica through tissue culture. Background technique [0002] Xiuzhu ( Bambusa glaucophylla ) belongs to the genus Bougainvillea, also known as Bougainvillea versicolor, is one of the important ornamental bamboos with slender leaf shape and excellent plant shape as ornamental characteristics. It is often used to make bonsai and garden cultivation for ornamental purposes. The bamboo is 300-500cm high and 2.5cm in diameter. It is a famous ornamental bamboo species and has broad market prospects. However, the flowering interval of bamboo plants is long, and they seldom bloom and set fruit. Because the pollen germination rate of bamboo plants is generally low, it is very difficult to obtain seeds. At present, bamboo propagation is mainly based on traditional methods such as transp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 林树燕刘国华丁雨龙郭婷婷赵荣魏强张春霞
Owner 扬州大禹风景竹园
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