A method for rapid propagation of air pineapple seedlings through leaf tissue culture

A technology for air pineapple and seedlings, which is applied in the field of rapid propagation of air pineapple seedlings, which can solve the problems of different experimental results and effects, and achieve the effects of quick and convenient material collection, fast growth of seedlings, and simple operation

Active Publication Date: 2021-09-07
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that the above-mentioned reports are all different from the present invention in aspects such as explants, culture medium formula ratio and method technology, and its experimental results and effects are also greatly (or completely) different.

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  • A method for rapid propagation of air pineapple seedlings through leaf tissue culture
  • A method for rapid propagation of air pineapple seedlings through leaf tissue culture
  • A method for rapid propagation of air pineapple seedlings through leaf tissue culture

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: Selection and disinfection of air pineapple leaves

[0026] 1. The collection of air pineapple leaf explants: the best time to collect materials is sunny, breezy, and cool morning; the preferred varieties are: tricolor flower (Tillandsia linen) T.tricolor, Becky T.Bergeri, Harris T.Harrisii, large Tricolor T.juncea, Elven T.Ionantha, etc. The selected plants should grow robustly without any pests and diseases. In order to ensure a high survival rate, the knife for cutting leaves needs to be sterilized at high temperature in advance. Return to the laboratory for disinfection.

[0027] 2. Disinfection and sterilization of leaf explants: Soak the leaf explants of T. juncea in washing powder solution for 5-8 minutes, and then rinse them in running water for 30-50 minutes. Then use 1% sodium hypochlorite + 2-3 drops of Tween to make a mixed disinfectant solution for 5-8 minutes, rinse with sterilized water for 3 times, then absorb the surface moisture, take i...

Embodiment 2

[0031] The induction differentiation of embodiment 2 clustered buds and the rapid propagation and proliferation of buds are cultivated

[0032] 1. Induced differentiation culture of leaf cluster buds: Transfer the sterile leaf explants obtained in the above-mentioned embodiment 1 to the leaf cluster bud induction differentiation medium, and cultivate them for 28 or 56 days under visible light for 14 h / d to induce leaf cluster buds production, and observe the growth and development of clustered buds, the results can be found in figure 2 and Table 2.

[0033] Table 2 Effects of clustering bud induction differentiation medium formula on the growth and development of clustering buds

[0034]

[0035] It can be seen from Table 2 that except for Y01, which is a control, no cluster buds occurred due to the influence of the starting medium formula, all other serial numbers can induce cluster buds on the cluster bud induction medium, but the difference in the degree of cluster bud...

Embodiment 3

[0041] Strong seedling rooting culture and transplanting of embodiment 3 seedlings

[0042] 1, rooting culture of strong seedlings: the rapid propagation sprouts that above-mentioned embodiment 2 obtains is divided into the strain group of 2-3 strains and changes into strong seedlings or strong seedling rooting medium, cultivates 14h / d under 2000-2500LX light, 28 days Left and right small sprouts just can grow into the normal robust plant ( Figure 4 ). According to the situation of the sprouts, the ones with better growth conditions are directly transferred to the strong seedling medium for cultivation, and the weaker ones are first cultivated in the strong seedling rooting medium for several days, and then moved into the strong seedling medium for cultivation. The formulations of seedling rooting medium are: Ms+6-BA0.5mg / L+IBA0.2mg / L; or: Ms+6-BA0.1mg / L+IBA0.5mg / L.

[0043] 2. Transplanting and survival of sterile seedlings: Uncover the bottle sealing film of the above-men...

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Abstract

The invention discloses a method for rapidly propagating air pineapple seedlings through leaf tissue culture, which comprises the following steps: 1) collection of air pineapple leaf explants; 2) disinfection and sterilization of leaf explants; 3) induction of clustered buds of leaf explants; 4 ) Rapid propagation and differentiation culture of sprouts; 5) Rooting culture of strong seedlings; 7) Hardening and transplanting of sterile seedlings. The present invention has the advantages of wide source of materials, fast and convenient material collection, simple operation, high propagation coefficient, fast growth of seedlings, rapid multiplication in a relatively short period of time, and a large number of tissue culture seedlings with consistent traits, consistent growth and robustness, etc., which not only solves the problem of air It is difficult to propagate pineapple seeds, the offspring may mutate, and cannot maintain the excellent traits of the female parent. It also solves the problem that many rare species or varieties will only produce rare daughter plants after flowering, which cannot meet the needs of tissue culture and rapid propagation. Realistic problems of solid materials.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for rapid propagation of pineapple seedlings through leaf tissue culture. Background technique [0002] Air pineapple is a kind of root-reduced plant that does not need to be planted in the soil, does not need roots to absorb water and fertilizer, but only absorbs water and nutrients in the atmosphere through its leaves, and can satisfy its own growth and development. It has a wide variety of plant types and shapes, in various poses and with different expressions, colorful flowers and leaves, and is very individual and beautiful. Air pineapple not only has the advantages of strong ornamental value and good decorative effect, but also has the ability to absorb formaldehyde, styrene, CO 2 And other powerful air purification function. Because of these characteristics, it is more and more favored by people as an ornamental plant, and its potential business opportunities are i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁慧敏梁栋李成龙韩鹏贾思振张文博张颖夏阳
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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