A method for rapid propagation of air pineapple seedlings through leaf tissue culture
A technology for air pineapple and seedlings, which is applied in the field of rapid propagation of air pineapple seedlings, which can solve the problems of different experimental results and effects, and achieve the effects of quick and convenient material collection, fast growth of seedlings, and simple operation
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Embodiment 1
[0025] Embodiment 1: Selection and disinfection of air pineapple leaves
[0026] 1. The collection of air pineapple leaf explants: the best time to collect materials is sunny, breezy, and cool morning; the preferred varieties are: tricolor flower (Tillandsia linen) T.tricolor, Becky T.Bergeri, Harris T.Harrisii, large Tricolor T.juncea, Elven T.Ionantha, etc. The selected plants should grow robustly without any pests and diseases. In order to ensure a high survival rate, the knife for cutting leaves needs to be sterilized at high temperature in advance. Return to the laboratory for disinfection.
[0027] 2. Disinfection and sterilization of leaf explants: Soak the leaf explants of T. juncea in washing powder solution for 5-8 minutes, and then rinse them in running water for 30-50 minutes. Then use 1% sodium hypochlorite + 2-3 drops of Tween to make a mixed disinfectant solution for 5-8 minutes, rinse with sterilized water for 3 times, then absorb the surface moisture, take i...
Embodiment 2
[0031] The induction differentiation of embodiment 2 clustered buds and the rapid propagation and proliferation of buds are cultivated
[0032] 1. Induced differentiation culture of leaf cluster buds: Transfer the sterile leaf explants obtained in the above-mentioned embodiment 1 to the leaf cluster bud induction differentiation medium, and cultivate them for 28 or 56 days under visible light for 14 h / d to induce leaf cluster buds production, and observe the growth and development of clustered buds, the results can be found in figure 2 and Table 2.
[0033] Table 2 Effects of clustering bud induction differentiation medium formula on the growth and development of clustering buds
[0034]
[0035] It can be seen from Table 2 that except for Y01, which is a control, no cluster buds occurred due to the influence of the starting medium formula, all other serial numbers can induce cluster buds on the cluster bud induction medium, but the difference in the degree of cluster bud...
Embodiment 3
[0041] Strong seedling rooting culture and transplanting of embodiment 3 seedlings
[0042] 1, rooting culture of strong seedlings: the rapid propagation sprouts that above-mentioned embodiment 2 obtains is divided into the strain group of 2-3 strains and changes into strong seedlings or strong seedling rooting medium, cultivates 14h / d under 2000-2500LX light, 28 days Left and right small sprouts just can grow into the normal robust plant ( Figure 4 ). According to the situation of the sprouts, the ones with better growth conditions are directly transferred to the strong seedling medium for cultivation, and the weaker ones are first cultivated in the strong seedling rooting medium for several days, and then moved into the strong seedling medium for cultivation. The formulations of seedling rooting medium are: Ms+6-BA0.5mg / L+IBA0.2mg / L; or: Ms+6-BA0.1mg / L+IBA0.5mg / L.
[0043] 2. Transplanting and survival of sterile seedlings: Uncover the bottle sealing film of the above-men...
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