Whitening polypeptide composition and preparation method thereof

Abstract

The invention relates to a whitening polypeptide composition and a preparation method thereof. The composition comprises polypeptide prepared from streptomyces griseus through a fermentation process, a radix sophorae flavescentis extract, an alpine aster flower extract, glycerol, sodium hyaluronate and a preservative. The preparation method of the polypeptide comprises the following steps: inoculating streptomyces griseus into a culture medium containing trace elements such as trehalose, fish collagen and selenium, culturing under specific conditions, and performing centrifugation and ultrafiltration separation to obtain the polypeptide with the molecular weight of 500-2000Da. The composition can effectively integrate the whitening and skin care effects of various plant extracts, and a novel skin whitening solution is provided through the unique component proportion and preparation process of the composition.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetic peptides, and in particular to a peptide composition for whitening and a preparation method thereof. Background Art

[0002] In today's society, as material life improves, people are increasingly pursuing the efficiency and safety of whitening care products. Although there are various whitening products on the market, there are some that are over-advertised or use inappropriate ingredients (such as lead, mercury and hormones). Although these ingredients can quickly achieve whitening effects, long-term use may cause serious health effects. As consumers' awareness of product safety and effectiveness increases, the demand for efficient and safe whitening products is increasing.

[0003] Currently, the mainstream whitening ingredients in the market, such as arbutin, kojic acid, vitamin C, etc., are popular. However, these whitening ingredients are difficult to effectively penetrate the skin barrier, affecting the absorption and effect of the whitening ingredients.

[0004] Therefore, it is necessary to develop a novel polypeptide composition for whitening. Summary of the invention

[0005] The present application provides a whitening polypeptide composition and a preparation method thereof, so as to improve the skin whitening and skin care effects.

[0006] The present application provides a polypeptide composition for whitening, which comprises, by weight:

[0007] 10 peptides were prepared by microbial fermentation process using Streptomyces griseus from the genus Actinomycetes as the production strain;

[0008] 2 parts of Sophora flavescens extract;

[0009] 1 part of Aster alpinia flower extract;

[0010] Glycerin 5 parts;

[0011] 0.5 parts of sodium hyaluronate;

[0012] 0.5 parts of preservatives;

[0013] The balance is purified water;

[0014] Wherein, the polypeptide is prepared by the following steps:

[0015] Streptomyces griseus from the genus Actinomycetes was selected as the production strain;

[0016] The selected microorganism is inoculated into a culture medium, wherein the culture medium comprises, by weight, 10 parts of trehalose, 5 parts of fish collagen, 1 part of trace elements, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate, and the remainder is water and a pH regulator; wherein the trace element is selenium;

[0017] The culture was carried out for 72 hours under the condition of temperature controlled at 28°C and pH maintained at 6.8;

[0018] collecting the fermentation culture broth, and obtaining a supernatant containing the target polypeptide by centrifugal separation;

[0019] The supernatant was subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000Da.

[0020] Furthermore, the polypeptide composition is in the form of emulsion, essence, gel or cream.

[0021] Furthermore, the Sophora flavescens extract is a Sophora flavescens water extract or a Sophora flavescens alcohol extract.

[0022] Furthermore, the Aster alpinia flower extract is a water extract or an alcohol extract.

[0023] Furthermore, the pH adjuster is sodium hydroxide or citric acid.

[0024] The present application provides a method for preparing the polypeptide composition as described above, comprising the following steps:

[0025] Streptomyces griseus from the genus Actinomycetes was selected as the production strain;

[0026] The selected microorganism is inoculated into a culture medium, wherein the culture medium comprises, by weight: 10 parts of trehalose, 5 parts of fish collagen, 1 part of trace elements, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate, and the remainder is water and a pH regulator; wherein the trace element is selenium;

[0027] The culture was carried out for 72 hours under the condition of temperature controlled at 28°C and pH maintained at 6.8;

[0028] collecting the fermentation culture broth, and obtaining a supernatant containing the target polypeptide by centrifugal separation;

[0029] The supernatant was subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000Da;

[0030] The separated polypeptide is mixed with Sophora flavescens extract, Aster alpinia flower extract, glycerin, sodium hyaluronate and a preservative in the weight proportions specified in claim 1 to prepare the polypeptide composition.

[0031] Furthermore, the Sophora flavescens extract is a Sophora flavescens water extract or a Sophora flavescens alcohol extract.

[0032] Furthermore, the Aster alpinia flower extract is a water extract or an alcohol extract.

[0033] Furthermore, the pH adjuster is sodium hydroxide or citric acid.

[0034] Furthermore, the centrifugation is performed at 4°C to maintain the activity of the polypeptide.

[0035] The beneficial technical effects of the present invention include: (1) The combination of peptides produced by Streptomyces griseus, Sophora flavescens extract and Aster alpinia flower extract used in the present invention is designed to provide a strong whitening effect. These ingredients each have their own unique mechanism of action, which helps to reduce the synthesis of melanin, which is a major factor in the formation of skin color. The synergistic effect of these ingredients provides a more significant whitening effect than a single active ingredient formulation. (2) Through the fermentation process with controlled conditions (such as pH and temperature), the method adopted can better maintain the biological activity of the peptides than traditional extraction methods. This method ensures that the peptides remain active and effective, enhancing the overall performance of the whitening product. (3) The glycerin and sodium hyaluronate included in the formula not only support the skin barrier, but also provide a deep moisturizing effect. Glycerin is a well-known humectant that can attract moisture, while sodium hyaluronate can retain a large amount of moisture, helping to maintain the moisture of the skin and reduce the dryness and peeling phenomena common in whitening treatments. DETAILED DESCRIPTION

[0036] Many specific details are described in the following description to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways than those described herein, and those skilled in the art can make similar generalizations without violating the connotation of the present application, so the present application is not limited by the specific implementation disclosed below.

[0037] The present application provides a polypeptide composition for whitening, which comprises, by weight:

[0038] 10 peptides were prepared by microbial fermentation process using Streptomyces griseus from the genus Actinomycetes as the production strain;

[0039] 2 parts of Sophora flavescens extract;

[0040] 1 part of Aster alpinia flower extract;

[0041] Glycerin 5 parts;

[0042] 0.5 parts of sodium hyaluronate;

[0043] 0.5 parts of preservatives;

[0044] The balance is purified water;

[0045] Wherein, the polypeptide is prepared by the following steps:

[0046] Streptomyces griseus from the genus Actinomycetes was selected as the production strain;

[0047] The selected microorganism is inoculated into a culture medium, wherein the culture medium comprises, by weight, 10 parts of trehalose, 5 parts of fish collagen, 1 part of trace elements, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate, and the remainder is water and a pH regulator; wherein the trace element is selenium;

[0048] The culture was carried out for 72 hours under the condition of temperature controlled at 28°C and pH maintained at 6.8;

[0049] collecting the fermentation culture broth, and obtaining a supernatant containing the target polypeptide by centrifugal separation;

[0050] The supernatant was subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000Da.

[0051] First, Streptomyces griseus is selected as a production strain, which is widely used in the field of biopharmaceuticals to produce a variety of effective biologically active substances. A suspension of Streptomyces griseus accounting for 1% of the volume of the culture medium can be inoculated into the culture medium.

[0052] Streptomyces griseus is inoculated in a special culture medium, the composition of which includes 10 parts of trehalose, 5 parts of fish collagen, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate. In addition, 1 part of trace element selenium is added, and an appropriate amount of water and pH regulator are used to adjust the culture medium to an ideal growth environment. The pH value of the culture medium is maintained at 6.8, the temperature is controlled at 28°C, and the culture is continued for 72 hours. Among them, the pH regulator is sodium hydroxide or citric acid. For example, 80 parts of water and 2 parts of sodium hydroxide can be added. It is also possible to choose to add an appropriate amount of sodium hydroxide or citric acid based on the measurement results of the pH value of the culture medium so that the pH value of the culture medium is maintained at 6.8. Here, fish collagen can be extracted based on tuna skin.

[0053] After the culture is completed, the supernatant in the fermentation culture is collected by centrifugation, which contains the target polypeptide. The supernatant is then subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000 Da. These polypeptides are the main active ingredients for the whitening effect in this example.

[0054] Next, the extracted polypeptide is mixed with 2 parts of Sophora flavescens extract and 1 part of Aster alpina flower extract in proportion. Sophora flavescens and Aster alpina flower extracts are both traditional Chinese medicinal materials, containing a variety of chemical components beneficial to the skin, which can promote skin whitening and improve skin resistance. 5 parts of glycerin and 0.5 parts of sodium hyaluronate are added as moisturizing ingredients to improve the water retention of the skin and make the skin smoother and more delicate. 0.5 parts of preservatives are added to ensure the long-term stability of the product. Purified water can be added to adjust the total amount to 100 parts to ensure uniform mixing and suitable for use as a skin care product. Among them, the preservative can be one or more of phenoxyethanol, parabens or isothiazolinone.

[0055] The polypeptide composition provided in the present application can be prepared into a variety of dosage forms, including emulsions, essences, gels or creams. The selection of these dosage forms allows the polypeptide composition to provide corresponding care effects according to different usage requirements and skin types. Emulsions are suitable for daily moisturizing and light care, essences are more suitable for targeted intensive treatment, gels are usually suitable for oily or mixed skin, and creams are suitable for dry or mature skin, which requires stronger moisturizing and nutrition.

[0056] To prepare peptide compositions in various dosage forms, such as lotions, serums, gels or creams, detailed formulations and process steps are required. The following is a general process for preparing various dosage forms:

[0057] The preparation of emulsions usually involves an oil-water emulsification process, in which the oil phase and the water phase are heated to the same temperature (usually about 70°C), and then the oil phase is slowly added to the water phase, stirring while adding until it is completely emulsified. The addition of peptides and other active ingredients during the emulsification process usually occurs after the temperature is slightly lowered to protect these sensitive ingredients from being damaged by high temperatures. Finally, other auxiliary ingredients such as preservatives and fragrances are added during the cooling process.

[0058] Essences are usually water-based and contain a high concentration of active ingredients. When preparing an essence, water is heated to an appropriate temperature and peptides, vitamins and other water-soluble ingredients are gradually dissolved. In order to increase the stability and feel of the product, it may also be necessary to add an appropriate amount of a thickener or colloid. The pH adjustment of the essence and the addition of preservatives are usually carried out after the product has almost cooled to room temperature.

[0059] The preparation of gel involves forming an appropriate gel network structure, usually using natural or synthetic colloids such as carboxymethyl cellulose or hydroxyethyl cellulose. First, prepare the water phase, gradually add the colloid powder after heating, and ensure full dispersion and dissolution under high-speed stirring. After the colloid is completely fused, add the peptide and other active ingredients, and adjust the pH and add preservatives in the final stage.

[0060] Creams are prepared similarly to lotions, but usually contain a higher proportion of oil phase ingredients, giving the final product a richer texture and stronger moisturizing ability. During the emulsification process, it is key to ensure that the oil phase and the water phase are thoroughly emulsified. The temperature control of active ingredient addition and the stirring after emulsification need to be precisely controlled to ensure product quality and stability.

[0061] When preparing these different dosage forms of products, it is crucial to ensure the cleanliness and sterility of all equipment and containers to avoid product contamination. In addition, necessary quality control tests such as pH test, stability test and microbiological test are carried out after each batch of products is completed to ensure the quality and safety of the products.

[0062] Through the above steps, various dosage forms of polypeptide compositions that meet specific needs and applications can be prepared, providing consumers with diverse choices.

[0063] In addition, the Sophora flavescens extract described in the present application may be a water extract or an alcohol extract. Different extraction methods may affect the extraction efficiency of the active ingredients in Sophora flavescens and the stability of the final product. Generally, water extracts are milder and suitable for sensitive skin, while alcohol extracts may contain more active ingredients and are suitable for products that require stronger effects.

[0064] Aster alpinum flower extract can also be extracted with water or alcohol. This extract is used in cosmetics for its antioxidant and anti-inflammatory properties, and different extraction solvents will affect its chemical composition and product suitability. Water extracts are generally more suitable for products used on sensitive skin, while alcohol extracts may be used in products for non-sensitive skin types to utilize more of its beneficial ingredients.

[0065] The present application provides a method for preparing the aforementioned polypeptide composition, comprising the following steps:

[0066] Streptomyces griseus from the genus Actinomycetes was selected as the production strain;

[0067] The selected microorganism is inoculated into a culture medium, wherein the culture medium comprises, by weight: 10 parts of trehalose, 5 parts of fish collagen, 1 part of trace elements, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate, and the remainder is water and a pH regulator; wherein the trace element is selenium;

[0068] The culture was carried out for 72 hours under the condition of temperature controlled at 28°C and pH maintained at 6.8;

[0069] collecting the fermentation culture broth, and obtaining a supernatant containing the target polypeptide by centrifugal separation;

[0070] The supernatant was subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000Da;

[0071] The separated polypeptide is mixed with Sophora flavescens extract, Aster alpinia flower extract, glycerin, sodium hyaluronate and a preservative in the weight proportions specified in claim 1 to prepare the polypeptide composition.

[0072] First, Streptomyces griseus in the genus Actinomycetes is selected as the production strain because it is known that Streptomyces griseus can produce a variety of beneficial bioactive substances, including polypeptides with whitening effects. A suspension of Streptomyces griseus accounting for 1% of the volume of the culture medium can be selected and inoculated into the culture medium.

[0073] During the inoculation process, Streptomyces griseus is inoculated into a specific culture medium, which consists of 10 parts of trehalose, 5 parts of fish collagen, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate. In addition, 1 part of trace element selenium is added, and an appropriate amount of water and pH regulator are used to adjust the culture medium to an ideal growth environment. These ingredients together support the growth and metabolic activities of the strain.

[0074] The trace element selenium is added to the culture medium as an important biocatalyst, which helps to promote the production of biologically active substances in Streptomyces. For example, 80 parts of water and 2 parts of sodium hydroxide can be added. It is also possible to choose to add an appropriate amount of sodium hydroxide or citric acid based on the measurement results of the pH value of the culture medium so that the pH value of the culture medium is maintained at 6.8. The pH value of the culture medium is maintained at 6.8, the temperature is controlled at 28°C, and the culture is continued for 72 hours. Among them, the pH regulator is sodium hydroxide or citric acid. For example, 80 parts of water and 2 parts of sodium hydroxide can be added. It is also possible to choose to add an appropriate amount of sodium hydroxide or citric acid based on the measurement results of the pH value of the culture medium so that the pH value of the culture medium is maintained at 6.8.

[0075] The cultivation process is carried out under controlled environmental conditions, in particular, the temperature is maintained at 28°C and the pH value is maintained at 6.8 for 72 hours to optimize the yield and quality of the polypeptide. After the cultivation is completed, the supernatant containing the target polypeptide is extracted from the culture broth by centrifugation, and then ultrafiltration is performed to accurately separate polypeptides with a molecular weight of 500-2000Da. The centrifugation is carried out at 4°C to maintain the activity of the polypeptide.

[0076] Finally, 10 parts of the extracted and refined peptides are mixed with 2 parts of Sophora flavescens extract, 1 part of Aster alpinia flower extract, 5 parts of glycerin, 0.5 parts of sodium hyaluronate and 0.5 parts of preservatives to form the final peptide composition. This composition not only contains effective whitening peptides, but also combines other natural ingredients with skin care effects, which work together to provide excellent whitening and skin quality improvement effects. Among them, the preservative can be phenoxyethanol, parabens or isothiazolinone.

[0077] Four embodiments using different ratios of purified water are provided below, and tests are performed to verify the effects. The four embodiments here all use the preparation method provided above to obtain the polypeptide composition.

[0078] Table 1: Four embodiments using different purified water ratios

[0079]

[0080] Table 1 provides four polypeptide compositions prepared according to the above polypeptide composition preparation method.

[0081] In order to verify the technical effect of the polypeptide composition, two test examples are provided below.

[0082] Test Example 1: Determination of melanin synthesis inhibition rate

[0083] 1. Preparation of experimental materials:

[0084] B16 melanoma cells were cultured using standard cell culture techniques using DMEM medium containing 10% fetal bovine serum.

[0085] Test samples: The polypeptide compositions of Examples 1 to 4 were each diluted to a concentration of 0.1 g / L, and DMEM culture medium was used as the dilution solution.

[0086] 2. Cell culture and sample processing:

[0087] B16 cells were cultured in a CO2 incubator at 37°C and 5% CO2 until they were nearly confluent.

[0088] For cell passaging, cells were treated with 0.25% trypsin.

[0089] The treated cells were adjusted to a concentration of 100,000 cells / mL, and 1 mL of each sample was inoculated in a 6-well plate.

[0090] After the cells adhered to the wall and grew stably, the culture medium was removed and the samples of each example were added, and the blank control group was added with the same volume of DMEM culture medium.

[0091] 3. Determination of melanin content:

[0092] After 72 hours of treatment, the cells were digested with 0.25% trypsin, washed with PBS and collected.

[0093] The cells were resuspended in 1 mol / L NaOH and reacted at 37°C for 48 hours to dissolve the melanin in the cells.

[0094] The supernatant sample was taken for absorbance measurement, and the wavelength was set to 475 nm.

[0095] 4. Data processing and statistical analysis:

[0096] The melanin synthesis inhibition rate was calculated using the formula:

[0097]

[0098] The following Table 2 shows the melanin synthesis inhibition effect of the polypeptide composition of each example, indicating the influence of different water contents on the whitening effect:

[0099] Table 2. Comparison of melanin synthesis inhibition effects of peptide compositions of various examples

[0100] Example No. Melanin inhibition rate (%) Example 1 86.28 Example 2 88.75 Example 3 90.16 Example 4 92.34

[0101] As can be seen from the above table, as the purified water content decreases, the inhibition rate of melanin synthesis gradually increases, showing that the formula with lower water content has stronger whitening activity, which is caused by the increase in the concentration of active ingredients.

[0102] Test Example 2: Whitening Efficacy Evaluation Test

[0103] 1. Subject Preparation

[0104] 340 volunteers aged between 30 and 50, each with at least two pigmented spots on their face, were selected and randomly assigned to 10 test groups, with approximately 34 people in each group.

[0105] 2. Test samples

[0106] Examples 1-4 and comparative examples 1-5 are included, and a group of placebos with no active ingredients are set up as a control.

[0107] Comparative Examples 1-5 have similar ingredients to the examples but do not contain the main active ingredient polypeptide. The purpose of this is to demonstrate the specific role of the key ingredient in the polypeptide composition. By comparison, the difference in whitening effect between the examples and the comparative examples can be seen.

[0108] The placebo group is a formula that does not contain any expected biologically active ingredients. It may be a simple basic lotion or gel base without any peptides or plant extracts. The use of placebo is to control psychological effects and other non-specific effects in the experiment and ensure the reliability and validity of the test results.

[0109] 2. Test equipment

[0110] The Visia skin analysis system (Canfield, USA) can accurately measure the color and spot changes of the skin.

[0111] 3. Evaluation indicators

[0112] The whitening effect of each sample was evaluated mainly by measuring the changes in the chromaticity parameters (L value) of the volunteers' skin before and after treatment. The higher the L value, the closer the skin is to pure white, and the greater the change, the more significant the whitening effect.

[0113] 4. Experimental Operation

[0114] Before the test began, the initial L* value of each subject was recorded. Then, the volunteers applied the sample assigned to them to their faces every morning and evening for 30 days. During the use period, the subjects were asked not to use any other whitening products or treatments.

[0115] 5. Record and analyze results

[0116] At the end of the experiment, the L value of each volunteer was measured again using the Visia skin analysis system and compared with the value before treatment. The percentage change of the L value of each volunteer was calculated and statistically analyzed to evaluate and compare the whitening effects of each embodiment.

[0117] Table 3. Comparison of whitening effects of peptide compositions of various examples

[0118] Group Percentage change in L* value Comparison of results with placebo group Example 1 +12％ Significantly better than placebo Comparative Example 1 +5.84％ Better than placebo Example 2 +13.67％ Significantly better than placebo Comparative Example 2 +6.37％ Better than placebo Example 3 +15.33％ Significantly better than placebo Comparative Example 3 +7.45％ Better than placebo Example 4 +17.67％ Significantly better than placebo Comparative Example 4 +8.95％ Better than placebo Placebo group +0.21％

[0119] In Table 3, the percentage change of L* value refers to the relative change of L* value from before treatment to after treatment, and the calculation formula is:

[0120]

[0121] The results compared with the placebo group illustrate the superiority or inferiority of the effect of each example relative to the placebo group without any active ingredient added.

[0122] The percentage change in L* value shows the relative change in skin brightness before and after treatment of the examples and comparative examples. For example, Example 1 shows a 10% increase in brightness, which means that its whitening effect is significant because the L* value increases by a relatively large percentage.

[0123] The L value change of the placebo group was almost zero (0.12%), which indicates that the placebo group had almost no whitening effect. In contrast, all embodiments and comparative examples showed an effect that exceeded that of the placebo group, but the improvement of the embodiments was more significant. For example, the percentage change of the L value of Example 4 reached 16.67%, which was much higher than 8.95% of Comparative Example 4 and 0.21% of the placebo group.

[0124] The effects of the embodiments are generally better than those of the comparative examples, which is attributed to the active polypeptides contained in the embodiments, which play a significant role in whitening. From the data, the embodiments have stronger whitening ability, showing the importance of the main active ingredient (polypeptide). The high performance of the polypeptides in the embodiments may be related to their effects on the activity of skin melanocytes. The polypeptides can inhibit the production of melanin, thereby leading to an increase in the L* value, i.e., an increase in skin brightness.

[0125] Through the above test examples 1 and 2, and by comparing the experimental effects of existing patents (such as CN109998938B, etc.), it can be seen that the combination of polypeptides produced by Streptomyces griseus, Sophora flavescens extract and Aster alpinia flower extract used in this application provides a strong whitening effect. These ingredients each have their own unique mechanism of action, which helps to reduce the synthesis of melanin, which is the main factor in the formation of skin color. The synergistic effect of these ingredients produces an unexpected technical effect, providing a more powerful whitening effect, thereby providing a new solution.

[0126] Although the present application is disclosed as above in the form of a preferred embodiment, it is not intended to limit the present application. Any technical personnel in this field may make possible changes and modifications without departing from the spirit and scope of the present application. Therefore, the scope of protection of the present application shall be based on the scope defined by the claims of the present application.

Claims

1. A polypeptide composition for whitening, It is characterized in that In parts by weight, the polypeptide composition comprises: 10 peptides were prepared by microbial fermentation process using Streptomyces griseus from the genus Actinomycetes as the production strain; 2 parts of Sophora flavescens extract; 1 part of Aster alpinia flower extract; Glycerin 5 parts; 0.5 parts of sodium hyaluronate; 0.5 parts of preservatives; The balance is purified water; Wherein, the polypeptide is prepared by the following steps: Streptomyces griseus from the genus Actinomycetes was selected as the production strain; The selected microorganism is inoculated into a culture medium, wherein the culture medium comprises, by weight, 10 parts of trehalose, 5 parts of fish collagen, 1 part of trace elements, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate, and the remainder is water and a pH regulator; wherein the trace element is selenium; The culture was carried out for 72 hours under the condition of temperature controlled at 28°C and pH maintained at 6.8; collecting the fermentation culture broth, and obtaining a supernatant containing the target polypeptide by centrifugal separation; The supernatant was subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000Da.

2. The polypeptide composition according to claim 1, It is characterized in that The dosage form of the polypeptide composition is emulsion, essence, gel or cream.

3. The polypeptide composition according to claim 1, It is characterized in that The sophora flavescens extract is a sophora flavescens water extract or a sophora flavescens alcohol extract.

4. The polypeptide composition according to claim 1, It is characterized in that The Aster alpinia flower extract is a water extract or an alcohol extract.

5. The polypeptide composition according to claim 1, It is characterized in that The pH adjuster is sodium hydroxide or citric acid.

6. A method for preparing the polypeptide composition according to claim 1, It is characterized in that The following steps are involved: Streptomyces griseus from the genus Actinomycetes was selected as the production strain; The selected microorganism is inoculated into a culture medium, wherein the culture medium comprises, by weight: 10 parts of trehalose, 5 parts of fish collagen, 1 part of trace elements, 10 parts of glucose, 2 parts of yeast extract, 1.5 parts of ammonium nitrate, 0.5 parts of dipotassium hydrogen phosphate and 0.2 parts of magnesium sulfate, and the remainder is water and a pH regulator; wherein the trace element is selenium; The culture was carried out for 72 hours under the condition of temperature controlled at 28°C and pH maintained at 6.8; collecting the fermentation culture broth, and obtaining a supernatant containing the target polypeptide by centrifugal separation; The supernatant was subjected to ultrafiltration to separate polypeptides with a molecular weight of 500-2000Da; The separated polypeptide is mixed with Sophora flavescens extract, Aster alpinia flower extract, glycerin, sodium hyaluronate and a preservative in the weight proportions specified in claim 1 to prepare the polypeptide composition.

7. The preparation method according to claim 6, It is characterized in that The sophora flavescens extract is a sophora flavescens water extract or a sophora flavescens alcohol extract.

8. The preparation method according to claim 6, It is characterized in that The Aster alpinia flower extract is a water extract or an alcohol extract.

9. The preparation method according to claim 6, It is characterized in that The pH adjuster is sodium hydroxide or citric acid.

10. The preparation method according to claim 6, It is characterized in that The centrifugation was performed at 4°C to maintain the activity of the polypeptide.

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