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A soil bio-improvement agent for continuous cropping of facility vegetables and its preparation and use method

A bio-improvement agent and soil biology technology, applied in the field of soil bio-improvement agents, can solve the problems of serious soil-borne diseases, increased pathogenic bacteria content, and decreased crop yield, so as to improve soil microbial groups, promote crop growth, and have a long storage period. Effect

Active Publication Date: 2021-05-07
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of pathogenic bacteria such as Fusarium and Phytophthora in the soil of continuous cropping greenhouse vegetables in Jiangsu, Shandong and other places has increased significantly, and soil-borne diseases have occurred seriously, resulting in a decrease in crop yield and economic benefits

Method used

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  • A soil bio-improvement agent for continuous cropping of facility vegetables and its preparation and use method
  • A soil bio-improvement agent for continuous cropping of facility vegetables and its preparation and use method
  • A soil bio-improvement agent for continuous cropping of facility vegetables and its preparation and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The synergistic antagonism of Bacillus subtilis and Bacillus megaterium on the soil-borne pathogenic fungus Fusarium wilt was determined by Sun Yunpei's (1960) method of co-toxicity coefficient of combined action of pesticides. Accurately pipette Bacillus subtilis (10 11 cfu / g) and Bacillus megaterium (10 11 / mL) bacterial liquid, according to Bacillus subtilis: Megaspore citrus ratio is 1: 0, 1: 0.5, 1: 1, 0.5: 1, 0: 1 different ratios prepare mixed bacterial liquid. On the ultra-clean workbench, 5 kinds of bacterial solutions with different proportions were diluted with sterile water into 5 drug solutions with concentration gradients, and 4mL of the drug solution was drawn from low concentration to high concentration with a micropipette, and added to the previously melted and sterilized solution respectively. Shake well, then pour an equal amount into four sterilized petri dishes with a diameter of 9 cm, and after cooling, the drug-containing culture of the correspon...

Embodiment 2

[0032] In the reactor, add 5% Bacillus subtilis fermentation concentrate according to the synergistic combination ratio of Bacillus subtilis in Example 1: Bacillus megaterium=1: 1 (number of viable bacteria ≥ 10 11 cfu / g), 5% Bacillus megaterium fermentation concentrate (the number of viable bacteria ≥ 10 11 cfu / g), while adding 0.1% glucose, 1% corn steep liquor dry powder and 2% humic acid, fully stirring until completely dissolved.

[0033] Add 10% straw biochar, 8% white carbon black and 15% rice bran into the mixer and mix evenly, then add the above-mentioned mixed bacterial solution and continue to stir and mix until the mixed bacterial solution is completely absorbed.

[0034] Add 2% starch, 51.9% diatomaceous earth and 12% water to the above mixture, continue to stir and mix, extrude into particles with a particle size of 3-5mm through a dry roller press granulator, and dry at 60°C in a fluidized bed , controlling the final water content of 12% to prepare soil bio-imp...

Embodiment 3

[0039] Effects of soil bio-amendrs on vegetable growth in indoor pot experiments. Take a 30cm soil sample from the inner surface of the facility vegetable greenhouse, crush and mix it evenly, and divide it into 3 equal parts, one of which is directly filled into 10 pots with a diameter of 10cm and a height of 15cm for comparison, and one part is compared with 2% of the implementation case 2 The soil bio-improvement agent was mixed evenly and filled into 10 pots of the same size. After 1 portion of soil sample was filled into 1 pot, 5 g of the above-mentioned soil bio-improvement agent was applied to the center of each pot, and then the roots were mixed. Sow 3 cucumber seeds respectively in the above-mentioned pots and pots and water them thoroughly. After the seeds germinated and emerged, one cucumber seedling with similar growth and size was kept for thinning, and the growth of the plants was observed. After 20 days, samples were taken to measure the fresh weight of the abov...

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Abstract

The invention discloses a bio-improvement agent for continuous cropping soil of facility vegetables, which comprises Bacillus subtilis and Bacillus megaterium carried by straw biochar, white carbon black, rice bran, glucose and dry powder of corn steep liquor, humic acid, wheat starch and diatomite. Preparation method: (1) prepare Bacillus subtilis and Bacillus megaterium fermentation liquids respectively; (2) stir and mix the fermentation liquids of the two strains; (3) add glucose, dry corn steep liquor and humic acid, stir, dissolve and mix (4) Add straw biochar, white carbon black, and rice bran and continue to stir until absorbed and mixed evenly; (5) Finally, add wheat starch and diatomaceous earth to mix, and replenish water at the same time, and prepare it after extrusion granulation and drying . Method of use: mixed with base fertilizer, and mixed with transplanted roots. The invention has the characteristics of improving soil physical and chemical properties and microbial community structure, reducing soil biological toxicity, inhibiting the reproduction of soil-borne pathogenic bacteria, and promoting the growth of crops.

Description

technical field [0001] The invention relates to a soil bio-improvement agent for continuous cropping of facility vegetables, in particular to a soil bio-improvement agent based on straw biochar carrying two kinds of soil beneficial microorganisms, which can improve the soil of facility vegetable continuous cropping and control soil-borne diseases, and the The preparation and use method of soil bio-improvement agent are described. Background technique [0002] In recent years, the area of ​​soil pollution in my country has continued to expand, and the degree of pollution has become increasingly serious. The 2014 "National Soil Pollution Survey Bulletin" shows that the rate of soil points exceeding the standard in the country is 16.1%, of which the rate of cultivated land soil exceeding the standard is 19.4%. Vegetables are important economic crops in my country. Due to the high intensity of vegetable soil multiple cropping and the large amount of agricultural inputs, soil po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/40C09K101/00
CPCC09K17/40C09K2101/00
Inventor 徐健韩光杰徐彬刘琴李传明祁建杭陆玉荣
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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