Primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and kit

A technology of pathogenic bacteria and tomato, applied in the field of molecular biology, can solve problems such as complex operation, low identification accuracy, threat to tomato yield and quality, and achieve the effect of strong primer specificity and high detection accuracy

Inactive Publication Date: 2017-06-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the expansion of facility tomato planting area and the increase of planting years, soil-borne diseases caused by tomato neck rot and Fusarium wilt have become more and more serious obstacles to continuous cropping, posing a serious threat to the yield and quality of tomatoes. The Bottleneck of Safe Industrial Production of ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and kit
  • Primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] 1. Primer design

[0029] According to the conserved sequence of pgx4 in GenBank (GenBank numbers AB256820.1, AB256830.1, AB256818.1, AB256798.1, AB256796.1, AB256797.1, AB256795.1, GU169176.1, AB256825.1), degenerate design Primer FL-F (5'-GATGGTGGAACGGTATGACYC-3', shown in SEQ ID NO: 5) and FL-R (5'-GATATCCTTRACACCATCACA-3', shown in SEQ ID NO: 6), the amplification length is 960bp, for The fragments were cloned, sequenced and analyzed, and specific primers for the physiological race 1 of Fusarium oxysporum tomato neck rot and root rot specialization and Fusarium oxysporum tomato specialization 1 were designed within the 960bp sequence.

[0030] The specific primer sequences for Fusarium oxysporum tomato neck rot and root rot are as follows:

[0031] ForL-upstream primer: 5'-CCTTACTCGACATTTTTTCAGA-3' (shown in SEQ ID NO: 1);

[0032] ForL-downstream primer: 5'-CATCAAGGCATAGTAAGAT-3' (shown in SEQ ID NO: 2);

[0033] SEQ ID NO: 1 consists of 21 bases, SEQ ID NO: 2 c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses primers for identifying pathogenic bacteria of tomato neck-root rot and wilt and a kit. The primer sequences for identifying pathogenic bacteria of tomato neck-root rot are shown as SEQ ID NO: 1 and SEQ ID NO: 2, and the primer sequences for identifying pathogenic bacteria of tomato wilt are shown as SEQ ID NO: 3 and SEQ ID NO: 4. The primers are strong in specificity and high in detection accuracy, can be used for detecting No. 1 physiological races of fusarium oxysporum tomato neck-root rot specialis and fusarium oxysporum tomato wilt specialis, and is significant for early quick detection of tomato neck-root rot and wilt, soil bacteria community distribution as well as prevention and control of diseases.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a primer and a kit for identifying pathogenic bacteria of tomato neck rot and fusarium wilt. Background technique [0002] Tomato (Solanum esculentum L.) is one of the most important vegetables in my country. It is estimated that the national tomato planting area is more than 10 million mu every year, and the output value is more than 40 billion yuan. Tomato planting area continues to expand, and now it can be planted year-round, and the yield is getting higher and higher, the varieties are more and more colorful, and it has a wide range of uses, which is deeply loved by growers and consumers. At present, the factors restricting tomato production are difficult to predict the market conditions, and the most important ones are serious pests and diseases, which make it difficult to improve the yield and quality of tomatoes. Among them, tomato neck rot (Fusarium crown and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12N15/11C12Q1/04C12R1/77
CPCC12Q1/6895
Inventor 叶青静杨悦俭王荣青阮美颖周国治姚祝平李志邈万红建程远
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap