A Molecular Marker Primer for Distinguishing Pomfret Oval and Pomfret Bryce and Its Application
A technology of molecular markers and oval pomfret, which is applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc., can solve the problems of marine species mixing, achieve easy mastery, and solve marine germplasm mixing , The effect of strong primer specificity
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Embodiment 1
[0026] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:
[0027] 1) Select 5 individuals of Pomfret Oval and Pompano Brycei species collected from the coastal waters of Sanya, Hainan to form a sample group, and extract the DNA of the two sample groups respectively;
[0028] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;
[0029] The composition of described PCR reaction system is:
[0030] Add in order to the 0.2ml centrifuge tube:
[0031]
[0032] The PCR amplification program was pre-denaturation at 94°C for 2 min, (denaturation at 94°C for 15 sec, annealing at 50°C for 15 sec, extension at 72°C for 15 sec) x 38 cycles, extension at 72°C for 3 min, and storage at 4°C for long-term storage.
[0033] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populations to identify thei...
Embodiment 2
[0035] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:
[0036] 1) Select 5 individuals of pomfret ovata and pomfret pomfret seedlings collected from the coastal waters of Sanya, Hainan, respectively, to form a sample group, and extract the DNA of the two sample groups respectively;
[0037] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;
[0038] The composition of described PCR reaction system is:
[0039] Add in order to the 0.2ml centrifuge tube:
[0040]
[0041] The PCR amplification program was pre-denaturation at 94°C for 4 min, (denaturation at 94°C for 35 sec, annealing at 53°C for 30 sec, extension at 72°C for 15 sec) x 30 cycles, extension at 72°C for 10 min, and storage at 4°C for long-term storage.
[0042] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populati...
Embodiment 3
[0044] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:
[0045] 1) Select 5 individuals of pomfret ovata and pomfret pomfret seedlings collected from the coastal waters of Sanya, Hainan, respectively, to form a sample group, and extract the DNA of the two sample groups respectively;
[0046] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;
[0047] The composition of described PCR reaction system is:
[0048] Add in order to the 0.2ml centrifuge tube:
[0049]
[0050] The PCR amplification program was pre-denaturation at 94°C for 5 min, (denaturation at 94°C for 35 sec, annealing at 50°C for 35 sec, extension at 72°C for 35 sec) x 38 cycles, extension at 72°C for 10 min, and storage at 4°C for long-term storage.
[0051] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populati...
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