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A Molecular Marker Primer for Distinguishing Pomfret Oval and Pomfret Bryce and Its Application

A technology of molecular markers and oval pomfret, which is applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc., can solve the problems of marine species mixing, achieve easy mastery, and solve marine germplasm mixing , The effect of strong primer specificity

Inactive Publication Date: 2020-12-01
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above problems, the purpose of the present invention is to provide a molecular marker primer and application thereof for distinguishing oval pomfret and Bryce pomfret, which helps to solve problems such as marine species mixing, for pomfret breeding, pomfret resource protection, reasonable Use and provide technical support for biodiversity research

Method used

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  • A Molecular Marker Primer for Distinguishing Pomfret Oval and Pomfret Bryce and Its Application
  • A Molecular Marker Primer for Distinguishing Pomfret Oval and Pomfret Bryce and Its Application
  • A Molecular Marker Primer for Distinguishing Pomfret Oval and Pomfret Bryce and Its Application

Examples

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Embodiment 1

[0026] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:

[0027] 1) Select 5 individuals of Pomfret Oval and Pompano Brycei species collected from the coastal waters of Sanya, Hainan to form a sample group, and extract the DNA of the two sample groups respectively;

[0028] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;

[0029] The composition of described PCR reaction system is:

[0030] Add in order to the 0.2ml centrifuge tube:

[0031]

[0032] The PCR amplification program was pre-denaturation at 94°C for 2 min, (denaturation at 94°C for 15 sec, annealing at 50°C for 15 sec, extension at 72°C for 15 sec) x 38 cycles, extension at 72°C for 3 min, and storage at 4°C for long-term storage.

[0033] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populations to identify thei...

Embodiment 2

[0035] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:

[0036] 1) Select 5 individuals of pomfret ovata and pomfret pomfret seedlings collected from the coastal waters of Sanya, Hainan, respectively, to form a sample group, and extract the DNA of the two sample groups respectively;

[0037] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;

[0038] The composition of described PCR reaction system is:

[0039] Add in order to the 0.2ml centrifuge tube:

[0040]

[0041] The PCR amplification program was pre-denaturation at 94°C for 4 min, (denaturation at 94°C for 35 sec, annealing at 53°C for 30 sec, extension at 72°C for 15 sec) x 30 cycles, extension at 72°C for 10 min, and storage at 4°C for long-term storage.

[0042] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populati...

Embodiment 3

[0044] A molecular marker primer for distinguishing pomfret ovata and pomfret brinneri and its identification method:

[0045] 1) Select 5 individuals of pomfret ovata and pomfret pomfret seedlings collected from the coastal waters of Sanya, Hainan, respectively, to form a sample group, and extract the DNA of the two sample groups respectively;

[0046] 2) using the DNA extracted in step 1) as a template, and amplifying through the PCR reaction system with primer CSF and primer CSR respectively;

[0047] The composition of described PCR reaction system is:

[0048] Add in order to the 0.2ml centrifuge tube:

[0049]

[0050] The PCR amplification program was pre-denaturation at 94°C for 5 min, (denaturation at 94°C for 35 sec, annealing at 50°C for 35 sec, extension at 72°C for 35 sec) x 38 cycles, extension at 72°C for 10 min, and storage at 4°C for long-term storage.

[0051] 3) Purify and sequence the PCR products, and compare the sequencing results of the two populati...

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Abstract

An objective of the present invention is to provide a molecular marker primer for identifying Trachitotus ovatus and Trachinotus blochii and application thereof, which can help solve the problems of mixed marine species and the like, and provide technical support for pompano breeding, pompano resource conservation, rational use and biodiversity research. The molecular marker primer for identifying Trachitotus ovatus and Trachinotus blochii includes a primer CSF and a primer CSR, and specific sequences are as follows: CSF: GTCATACGCTCCCGAAGAT; and CSR: TAGGGCTAAGCATAGTGGG. The molecular marker primer is used for identifying Trachitotus ovatus and Trachinotus blochii; and a kit including the molecular marker primer and a PCR reaction system reagent is applied to the identification of Trachitotus ovatus and Trachinotus blochii. The application also includes that molecular markers are used for identifying Trachitotus ovatus and Trachinotus blochii, and the application also includes identifying fries thereof According to results, the two fishes have a difference of 2 bases, which is shown as 1 transformation and 1 transversion. The results are stable and the reproducibility is high.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a molecular marker primer for distinguishing pomfret seedlings from oval pomfret seedlings and pomfret carp seedlings and an application thereof. Background technique [0002] Both Trachinotus ovatus and Trachinotus blochii belong to the order Perciformes, family Carangidae, genus Trachinotus, and are widely distributed in the temperate regions of the Indian Ocean, the Pacific Ocean and the Atlantic Ocean. , Tropical waters. Both pomfret and pomfret are marine fish with high economic value. The cage culture of these two species of pomfret has been carried out since the 1990s along the coasts of Hainan, Guangdong and Fujian. Because the two kinds of pomfret are very similar in shape, they are called yellow pomfret, seawater white pomfret or golden pomfret in coastal areas of China. Although the two types of pomfret are very similar, the temperature adaptabi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124
Inventor 单斌斌孙典荣刘岩杨长平赵雨
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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