The invention relates to a method and kit for detecting a
wild strain of a classical fever
virus. The method for detecting the
wild strain of the
classical swine fever virus includes the following steps that (1), a detection sample is obtained, specifically,
wild strain RNA of the
classical swine fever virus is extracted, and the
RNA concentration is diluted to 10 copies / [mu]L after extraction; (2), a
reaction system is prepared, specifically, 7[mu]L of a constant temperature amplification
buffer solution, 1[mu]L of a constant temperature amplification
enzyme solution and 2[mu]L of a
RNA solution diluted in the step (1) are taken and mixed into 10 [mu]L of a reaction solution, and the reaction solution is evenly mixed through vortex vibration; (3),
nucleic acid sequence based amplification(
NASBA) and detection are carried out, specifically, the reaction solution is put into a real-time
fluorescent PCR instrument, an
NASBA primer group and a probe group are used, the temperature is setto be 41 DEG C, constant temperature amplification reaction is carried out for 1h, and meanwhile, real-time fluorescent scanning is completed; and (4) a result is judged, specifically, when a typicallying-S type amplification curve is detected, the detecting result is positive.