Detection method and a kit of transgenic soybean MON89788 transformation event

A technology of MON89788 and genetically modified soybean, which is applied in the field of detecting whether there is a transformation event of genetically modified soybean MON89788 in biological samples, which can solve the problems of increasing the complexity of the test and mutual contamination of the experimental results, and achieves the reduction of detection cost, mutual pollution and easy degradation. Effect
CN103409498AInactive Publication Date: 2013-11-27海康生物科技(北京)有限公司 +1
3 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
海康生物科技(北京)有限公司
Publication Date
2013-11-27
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a detection method and a kit of transgenic soybean MON89788 transformation event. The method comprises the following steps: performing high temperature degeneration on a sample DNA (deoxyribonucleic acid), transcribing under RNA (ribonucleic acid) polymerase by using a primer for identifying MON89788 specificity region to generate RNA, performing NASBA (nucleic acid sequence based amplification) by using the above primer based on obtained RNA as a template; and finally detecting the amplification product. Without purchasing large instrument, the detection cost of the method disclosed by the invention is reduced, the detected product is easy to degrade, and the problem of cross contamination among samples is reduced.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of transgenic plant detection, in particular to a method and a kit for detecting whether there is a transgenic soybean MON89788 transformation event in a biological sample. Background technique

[0002] The successful introduction of commercially interesting traits into plants by genetic manipulation in agriculture or industry is a complex process dependent on different factors. According to statistics from the International Agricultural Bioengineering Applied Technology Acquisition Authority (ISAAA), since the commercialization of genetically modified products, the planting area of ​​genetically modified crops has exceeded 1 billion hectares. China has become the sixth largest growth country of GM crops. With the management of genetically modified products in various countries and people's attention to the safety of genetically modified products, countries around the world have issued relevant laws and policies to r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More