2 results about "NASBA" patented technology

The invention discloses a universal probe for multiple nucleic acid detection, a probe combination and application of the universal probe and the probe combination, aiming at the limitation that the existing nucleic acid detection technology depends on expensive equipment, is complicated to operate and is difficult to realize multiple detection. A bridging detection system is formed by innovatively designing a general probe, a specific probe series A (two ends of which are fixed in sequence and are used for connecting the general probe and a quality control line) and a specific probe series B (one end of which is fixed on a detection line); the test strip structurally comprises a sample pad, a colloidal gold pad (for fixing a universal probe), an NC (nitrocellulose) membrane (containing a plurality of detection lines and quality control lines) and absorbent paper. The method is suitable for single-stranded nucleic acid (such as NASBA amplification products) detection, has high sensitivity, high specificity (no false positive), rapidness (completion within 10 minutes) and multiple detection capability, does not need complex instruments, is low in cost, and is easy to popularize in a basic level.

The application discloses a gene and drug resistance mutation detection method based on a double-site nucleic acid circuit probe and belongs to the technical field of molecular biology. In order to solve the technical problems of false positive detection results, complex operation, high requirements for detection personnel and high cost in the prior art gene and drug resistance mutation detection method, the application provides a gene and drug resistance mutation detection method based on a double-site nucleic acid circuit probe. The method is based on isothermal amplification technology combined with one or more nucleic acid circuits. The isothermal amplification technology includes but is not limited to NASBA, SDA, LAMP, HDA and RPA. The nucleic acid circuit includes but is not limited to a cascade strand displacement nucleic acid circuit and a one-step strand displacement nucleic acid circuit. The detection method provided by the application can simultaneously detect multiple drug resistance mutation sites through a single tube and a single reaction, realizes pathogen detection and pathogen drug resistance mutation detection on unknown samples, has high detection accuracy, is simple in steps and low in cost.