1 results about "NASBA" patented technology

The application discloses a gene and drug resistance mutation detection method based on a double-site nucleic acid circuit probe and belongs to the technical field of molecular biology. In order to solve the technical problems of false positive detection results, complex operation, high requirements for detection personnel and high cost in the prior art gene and drug resistance mutation detection method, the application provides a gene and drug resistance mutation detection method based on a double-site nucleic acid circuit probe. The method is based on isothermal amplification technology combined with one or more nucleic acid circuits. The isothermal amplification technology includes but is not limited to NASBA, SDA, LAMP, HDA and RPA. The nucleic acid circuit includes but is not limited to a cascade strand displacement nucleic acid circuit and a one-step strand displacement nucleic acid circuit. The detection method provided by the application can simultaneously detect multiple drug resistance mutation sites through a single tube and a single reaction, realizes pathogen detection and pathogen drug resistance mutation detection on unknown samples, has high detection accuracy, is simple in steps and low in cost.