51results about How to "Strong fluorescence response" patented technology

The invention discloses a rhodamine 6G hydrazide derivative, a preparation method of the derivative and an application of the derivative, and a method for carrying out fluorescence analysis on hypochlorous acid by using the derivative as a fluorescence probe. The rhodamine 6G hydrazide derivative is selectively reacted with hypochlorous acid to rapidly generate an obvious fluorescence enhancement effect. The rhodamine 6G hydrazide derivative disclosed by the invention is capable of realizing high-sensitivity and high-selectivity quantitative detection on trace hypochlorous acid in water and cells, and wide in application prospect in the fields of environment, biology and the like.

The invention discloses a fluorescent reagent for detecting specificity of aluminium ions based on an aggregation-induced emission principle. The fluorescent reagent is represented as sodium4-(2, 5-diphenyl-1H-pyrrole-1-yl)benzoate (TriPP-COONa). The fluorescent reagent has fluorescence enhancement specific response to the aluminium ions, has the detection limit of 1 mu M (27 mu g/ L) which is lower than the aluminium ion concentration (no higher than 200 mu g/ L) in drinking water stipulated by the World Health Organization, is free of the interference of other ions, such as K+, Mg2+, Ca2+, Ba2+, Ni2+, and has short response time. The fluorescence intensity of the fluorescent reagent has good linear relationship within the aluminium ion concentration range of 1 mu M to 11 mu M. According to the linear equation in the invention, the quantitive detection of the concentration of aluminium ions in the water sample can be realized conveniently and rapidly.

The invention discloses N-(2,4-dinitrophenyl)-rhodamine B hydrazide and a preparation method and an application of the N-(2,4-dinitrophenyl)-rhodamine B hydrazide. The rhodamine B derivative provided by the invention is N-(2,4-dinitrophenyl)-rhodamine B hydrazide, the structural formula of which is I. The N-(2,4-dinitrophenyl)-rhodamine B hydrazide, is colorless and free from fluorescence, but can selectively react with Cu<2+> ions in chromogenic and fluorescent opening reaction. The colorless substance generates a system with excellent optical performance, so that the detection sensitivity and selectivity of Cu<2+> ions are greatly improved. Therefore, the N-(2,4-dinitrophenyl)-rhodamine B hydrazide is appropriate for high selectivity and high sensitivity detection for the Cu<2+> ions. Detection can be carried out by a light absorption spectrum and a fluorescence spectrum. (FORMULA I).

The invention discloses a rhodamine B derivative containing an 8-aminoquinoline group, a preparation method, an application and a method for carrying out fluorescence analysis on Cr<3+> and Cu<2+>. The rhodamine B derivative containing the 8-aminoquinoline group is capable of selectively and quantitatively detecting chromium ions (Cr<3+>) in acetonitrile, and is capable of selectively and quantitatively detecting copper ions (Cu<2+>) in ethanol and generating an obvious fluorescence increment effect. The rhodamine B derivative containing the 8-aminoquinoline group is simple in synthesis method, and is capable of quantitatively detecting different heavy metal ions Cr<3+> and Cu<2+> in different solvents at high selectivity and high sensitivity, is a dual-functional fluorescence probe, and has a good application prospect in the fields of environment analysis and detection.

The invention discloses a macromolecular pH fluorescent probe for cotton transfer printing and dyeing. The structural formula of the macromolecular pH fluorescent probe is shown in the description. After 2-poistion Rhodamine B with carboxyl and hydrazine hydrate react to generate lactam, lactam reacts with acryloyl chloride, monomers with Rhodamine groups are obtained, and finally the macromolecular pH fluorescent probe with specific fluorescent responses to H+ is synthesized through copolymerization. The macromolecular pH fluorescent probe has very good water solubility, the fluorescent responses to the pH from alkaline to acidulous are very remarkable, meanwhile, the macromolecular pH fluorescent probe can achieve hot melting to be softened at the temperature of 110 DEG C, and can transfer and adsorb to the surfaces of cotton fibers, and therefore the reaction extent of the fabric surface can be tracked and monitored conveniently in the steaming process. Whether the reaction is finished or not can be known conveniently in real time by monitoring the pH value of the fabric surface through the pH fluorescent probe in the steaming process, and therefore the reaction endpoint is precisely controlled.

The invention discloses an enhanced azo Salen Schiff base fluorescent probe and synthesis and application thereof, belonging to the technical field of fluorescent probes. The chemical formula of the fluorescent probe is C39H34N8O7, and the structural formula of the fluorescent probe is as shown in the specification. The fluorescent probe has good selectivity on Al <3+>, good linear relation, highsensitivity, strong fluorescence response, low detection limit and low cost, and can be widely applied to detection of Al<3+> ions in an environment. Compared with the prior art, the fluorescent probeof the invention has the advantages that more than one time of coordination sites can be provided; the fluorescent probe is more easily coordinated with Al<3+> to form a binuclear complex with a plurality of coordination sites; and molecular plane rigidity is enhanced after coordination, so the photoinduced electron transfer effect of Schiff base nitrogen atoms is inhibited, and a strong chelating fluorescence enhancement effect is generated. The synthesis method of the probe is simple, and has high yield, and reaction conditions are easy to control.

The invention relates to an organic framework material for evaluating the jaundice level of a human body as well as a preparation method and application thereof. The method is characterized in that arare earth post-functionalized metal-organic framework material composed of zirconium tetrachloride and 1, 2, 4, 5-benzenetetracarboxylic acid Is adopted, and the preparation method comprises the following steps: preparing UiO-66(COOH)2 from the zirconium tetrachloride and the 1, 2, 4, 5-benzenetetracarboxylic acid by virtue of a heating reflux method, dispersing the UiO-66(COOH)2 in an aqueous solution of europium nitrate, stirring at room temperature to prepare an activated metal organic framework material UiO-66(COOH)2: Eu, and dropwise dispensing the metal-organic framework material on porous test paper through ethanol dispersion to obtain fluorescent test paper which can be used for measurement of bilirubin in human serum. Compared with the prior art, the organic framework material has the advantages that the content of bilirubin in human serum can be rapidly and effectively obtained through human eyes or color reading software, accurate and rapid detection of the content of bilirubin in human serum is achieved, operation is rapid, and the organic framework material has a potential application value in the field of material science.

The invention discloses a bis-Schiff base aluminum ion fluorescent probe as well as a synthesis method and application thereof, and belongs to the technical field of fluorescent probes. The chemical formula of the bis-Schiff base aluminum ion fluorescent probe is C23H18N2O4F3Cl, and the structural formula of the bis-Schiff base aluminum ion fluorescent probe is shown in the specification. The bis-Schiff base aluminum ion fluorescent probe has strong fluorescence response, good selectivity, high sensitivity and low detection limit on Al < 3 + >, can be recognized by naked eyes and can be widelyapplied to detection of Al < 3 + >. The synthesis method of the bis-Schiff base aluminum ion fluorescent probe is simple, high in yield and suitable for industrial production. According to a method for preparing 3, 5-diamino-4-chlorobenzotrifluoride, the reaction yield is high, the conditions are easy to control, the operation is simple, an organic solvent can be recycled, and the pollution is small.

The invention belongs to the technical field of small organic molecule fluorescent probes, particularly relates to a fluorescent probe for detecting hypochlorous acid, a preparation method and applications thereof, and provides a fluorescent probe, which has a structure represented by a formula (I). According to the present invention, the fluorescent probe has strong selectivity on hypochlorous acid, and can generate strong fluorescence response after being reacted with hypochlorous acid; when the fluorescence probe with a concentration of 5 [mu]M reacts with hypochlorous acid with a concentration of 0-150 [mu]M, the fluorescence response is enhanced along with the increase of the concentration of hypochlorous acid; and the fluorescence probe has advantages of high sensitivity, good selectivity and good response to hypochlorous acid.

The invention discloses an 8-aminoquinoline amide derivative, a preparation method, application and a fluorescence analysis method thereof. The preparation method of the 8-aminoquinoline amide derivative comprises the following steps that (1a) N-bromoacetyl-8-aminoquinoline, propargylamine and acetonitrile are weighed, the N-bromoacetyl-8-aminoquinoline, propargylamine and the acetonitrile are dissolved in the acetonitrile, and then potassium carbonate is added to obtain a reaction mixture; (2a) the reaction mixture obtained in the step (1a) is subjected to stirring reaction at room temperature, filtration is performed, a solvent is removed, and then the 8-aminoquinoline amide derivative is obtained through column chromatography elution. The 8-aminoquinoline amide derivative can be appliedto the field of fluorescent probes. Zn<2+> and Cd<2+> can be simultaneously detected through the derivative, the preparation method, application and the fluorescent analysis method performed based onthe derivative.

The invention discloses a kit for detecting bleomycin based on a double-ring hairpin probe and an enzyme-mediated cascade amplification strategy and a detection method of the kit. The double-ring hairpin probe can effectively silence a trigger sequence and inhibit non-specific hybridization, and therefore a background signal of the detection method is reduced; an enzyme-mediated cascade amplification reaction can achieve multiple amplification of a detection signal, therefore, the sensitivity of the detection method is improved, high-sensitivity detection of bleomycin is achieved, the linear range is 40 pM to 10 nM, and the detection limit is 35 pM. The sensitivity is improved by about two orders of magnitudes compared with a previously established label-free fluorescence detection method, and the requirements of clinical sample detection can be met.

The invention discloses a fluorescent reagent and a preparation method for stepwise detection of trace amounts of trivalent aluminum ions and divalent lead ions, belonging to the field of fluorescent chemical sensors. The fluorescent reagent consists of PPPNa and a good solvent. 3,4-dibromo-1,2,5-three (methoxycarbonylphenyl) pyrrole is dissolved in good solvent, add 4-methoxycarbonylphenylboronic acid, catalyst and basic salt, react to obtain PPP; PPP Dissolve in a solvent, add alkali, and react to obtain PPPNa. The PPPNa is dissolved in a good solvent to obtain a fluorescent reagent; the fluorescent reagent can quantitatively detect aluminum ions or divalent lead ions in aqueous solution or mixed salt solution, the preparation method is simple, the operation is convenient, the response is fast, and it has high sensitivity It can completely break away from the dependence on high-precision instruments according to actual needs, and will largely meet people's detection requirements for aluminum ions and lead ions today.