6results about How to "Strong fluorescence response" patented technology

The invention relates to a hydrazine hydrate molecular fluorescent probe as well as a preparation method and application thereof, and relates to the technical field of biochemical detection tools. The invention provides a hydrazine hydrate molecular fluorescent probe with a structural formula as shown in a formula (1): # imgabs0 #, and further provides a specific preparation method and application of the hydrazine hydrate molecular fluorescent probe on the basis of the hydrazine hydrate molecular fluorescent probe. The hydrazine hydrate molecular fluorescent probe provided by the invention is high in sensitivity, good in selectivity and low in detection limit, and can be successfully applied to monitoring of hydrazine hydrate in a water environment and a cell system.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

This invention discloses a nanosheet Tb-MOF fluorescence sensor for detecting Al in phosphogypsum and red mud. 3+ The detection application utilizes Tb(NO3)3·5H2O and H4BTTC as raw materials, with benzoic acid as a modifier, to prepare a Tb-BTTC MOF fluorescent sensor with a nanosheet structure and benzothiadiazole functionalization using a microwave-assisted synthesis method. This material can be used as a fluorescent sensor to detect Al in phosphogypsum and red mud. 3+ This invention utilizes highly selective, "on"-type fluorescence enhancement detection, accompanied by a significant blue shift (approximately 10 nm), with a fluorescence spectroscopy detection limit of 0.065 μM. It constructs an intelligent sensing platform integrating a smartphone and a portable UV lamp, achieving Al2O3 detection through fluorescence color and RGB analysis. 3+ The rapid on-site quantitative detection method can be applied to the rapid and visual detection of aluminum ions in solid wastes such as red mud and phosphogypsum, providing new materials and methods for the real-time and portable detection of aluminum ions in red mud and phosphogypsum.

The invention belongs to the field of fluorescent probes, and discloses a novel rhodamine Al < 3 + > fluorescent probe as well as a preparation method and application thereof. The method comprises the following steps: synthesizing an intermediate R from cyanuric chloride serving as a framework and p-hydroxybenzaldehyde, synthesizing rhodamine B hydrazide from rhodamine B and hydrazine hydrate under the protection of nitrogen, and finally synthesizing the final product probe R1 from the intermediate R and the rhodamine B hydrazide. After 10 times of KSCN is added into the probe R1 as a color developing agent, the interference of Fe < 3 + >, Cu < 2 + > and Zn < 2 + > ions is masked, and the probe R1 shows high sensitivity and selectivity to Al < 3 + >. Furthermore, the lowest detection limit of Al < 3 + > is calculated to be 0.1 mu mol / L through fluorescence titration test. Therefore, the probe R1 prepared by the invention can realize high-selectivity fluorescent recognition on Al < 3 + > ions, and can be used as a fluorescent probe for detecting metal ions to be applied to the related fields of environment detection, biomedical treatment, agriculture and the like.

The invention discloses fluorescent hydrogel microspheres for rapidly detecting tetracycline as well as a preparation method and a detection method of the fluorescent hydrogel microspheres. The fluorescent hydrogel microsphere is mainly prepared from sodium alginate and a fluorescent complex, wherein the sodium alginate is a carrier of the fluorescent complex; the fluorescent complex is mainly formed by self-assembling guanylic acid, a europium ion compound and luminol; the fluorescent hydrogel microspheres are formed by dropwise adding a mixed solution of sodium alginate and a fluorescent complex into a calcium salt solution and solidifying; the fluorescent hydrogel microspheres are added into a sample solution containing tetracycline, after a reaction is carried out for a period of time at room temperature in a dark place, quantitative detection of tetracycline is realized by utilizing the ratio fluorescence change of the fluorescent hydrogel microspheres, and the detection limit is 5 mu g / kg. The fluorescent hydrogel microsphere provided by the invention is simple to prepare and mild in synthesis condition, after the fluorescent hydrogel microsphere responds to tetracycline, the hue change is obvious, the change is visible to naked eyes, and the fluorescent hydrogel microsphere has the advantages of simplicity, convenience, rapidness, sensitivity and the like.

The invention discloses a double-color nanoprobe for detecting alkaline phosphatase as well as a preparation method and a detection method of the double-color nanoprobe. The bicolor nano-probe is mainly formed by self-assembly of guanylic acid, a terbium ion compound and a water-soluble carboxyl functionalized quantum dot at room temperature under a dark condition; the mass ratio of the guanylic acid to the terbium ions to the water-soluble carboxyl functionalized quantum dots is (750000 to 800000): (750000 to 800000): 1; the bicolor nanoprobe and a sample solution containing alkaline phosphatase are uniformly mixed, after a reaction in a dark place at 37 DEG C, the concentration of the alkaline phosphatase is obtained from a fluorescence emission peak value at 546 nm under the excitation wavelength of 254 nm, and the lowest concentration of the alkaline phosphatase in the detection method is 0.3874 U / L. The bicolor nanoprobe is simple in synthesis mode and mild in synthesis condition, the generated color contrast signal is visible to naked eyes, and the bicolor nanoprobe has the advantages of being simple, rapid and sensitive.