51results about How to "Strong fluorescence response" patented technology

In a sensor system, an active sensor chip includes an array of periodically-patterned dielectric active sensor patches of different periodicities and geometries formed on a metal film. A specimen under study is positioned on each patch, and the active sensor chip is interrogated by illumination the patches in a predetermined sequence to result in a fluorescence response from each patch enhanced by SPP. The intensity of the fluorescence response is controlled by varying the wavelength, incidence angle, azimuthal orientation and polarization direction of the excitation light beam as the function of the periodicity of the illuminated patch. The system is compatible with commercial fluorescence microscopes and scanned laser interrogation systems.

The invention discloses an organic ligand for synthesizing metal organic frames. The chemical nomenclature of the organic ligand is 2-(3, 5-bis (4-pyridyl) phenyl) benzoglioxaline. The chemical formula of the organic ligand is represented in the description. The invention also discloses a synthetic method of the organic ligand and a Cu (I) ion-based bright yellow metal organic frame (MOF-1) and a Cu (I) ion-based dark red metal organic frame (MOF-2), and the chemical formulas are {[Cu(L)I].0.5CH3CN.0.5CH3OH.0.75H2O}n, (n=1,2,3...'8') and {[Cu(L)I].2H2O}n(n=1,2,3...'8'). The invention also provides a synthetic method for dark red Cu (I) ion metal organic frame (MOF-2) and a synthetic method for organic matters of the Cu (I) ion metal organic frame. The invention also provides application of the organic ligand and metal organic frames serving as alcohols small organic molecule fluorescence sensing materials. The application operation is simple, the cost is low, the respond is quick, and the obvious fluorescence response for methanol, ethanol, isopropanol , n-propanol under the liquid phase and the gas phase can be achieved.

The invention discloses a rhodamine 6G hydrazide derivative, a preparation method of the derivative and an application of the derivative, and a method for carrying out fluorescence analysis on hypochlorous acid by using the derivative as a fluorescence probe. The rhodamine 6G hydrazide derivative is selectively reacted with hypochlorous acid to rapidly generate an obvious fluorescence enhancement effect. The rhodamine 6G hydrazide derivative disclosed by the invention is capable of realizing high-sensitivity and high-selectivity quantitative detection on trace hypochlorous acid in water and cells, and wide in application prospect in the fields of environment, biology and the like.

The invention discloses a fluorescent reagent for detecting specificity of aluminium ions based on an aggregation-induced emission principle. The fluorescent reagent is represented as sodium4-(2, 5-diphenyl-1H-pyrrole-1-yl)benzoate (TriPP-COONa). The fluorescent reagent has fluorescence enhancement specific response to the aluminium ions, has the detection limit of 1 mu M (27 mu g / L) which is lower than the aluminium ion concentration (no higher than 200 mu g / L) in drinking water stipulated by the World Health Organization, is free of the interference of other ions, such as K+, Mg2+, Ca2+, Ba2+, Ni2+, and has short response time. The fluorescence intensity of the fluorescent reagent has good linear relationship within the aluminium ion concentration range of 1 mu M to 11 mu M. According to the linear equation in the invention, the quantitive detection of the concentration of aluminium ions in the water sample can be realized conveniently and rapidly.

The invention discloses N-(2,4-dinitrophenyl)-rhodamine B hydrazide and a preparation method and an application of the N-(2,4-dinitrophenyl)-rhodamine B hydrazide. The rhodamine B derivative provided by the invention is N-(2,4-dinitrophenyl)-rhodamine B hydrazide, the structural formula of which is I. The N-(2,4-dinitrophenyl)-rhodamine B hydrazide, is colorless and free from fluorescence, but can selectively react with Cu<2+> ions in chromogenic and fluorescent opening reaction. The colorless substance generates a system with excellent optical performance, so that the detection sensitivity and selectivity of Cu<2+> ions are greatly improved. Therefore, the N-(2,4-dinitrophenyl)-rhodamine B hydrazide is appropriate for high selectivity and high sensitivity detection for the Cu<2+> ions. Detection can be carried out by a light absorption spectrum and a fluorescence spectrum. (FORMULA I).

The present invention relates to polymerisable fluorescent functional monomers in the structure as shown, and they have strong fluorescent reaction and part capable of reacting with biomacromolecular protein and non-covalent bond of DNA mutually. They may be polymerized with hydrophilic monomer to obtain fluorescent amphiphilic polymer with excellent biocompatibility, medicine carrying capacity and target administration capacity, and the polymer may find wide application foreground in high sensitivity fluorescent tracing detection technology, biomacromolecule separation and distinction, biosensor, medical diagnosis, medicine conveying, target material and other aspects. The present invention has reasonable synthesis path, simple synthesis process, easy-to-obtain material and high application value.

The invention discloses a rhodamine B derivative containing an 8-aminoquinoline group, a preparation method, an application and a method for carrying out fluorescence analysis on Cr<3+> and Cu<2+>. The rhodamine B derivative containing the 8-aminoquinoline group is capable of selectively and quantitatively detecting chromium ions (Cr<3+>) in acetonitrile, and is capable of selectively and quantitatively detecting copper ions (Cu<2+>) in ethanol and generating an obvious fluorescence increment effect. The rhodamine B derivative containing the 8-aminoquinoline group is simple in synthesis method, and is capable of quantitatively detecting different heavy metal ions Cr<3+> and Cu<2+> in different solvents at high selectivity and high sensitivity, is a dual-functional fluorescence probe, and has a good application prospect in the fields of environment analysis and detection.

A preparing method of N-(2,4-dinitrophenyl)rhodamine B-hydrazide and an application of the N-(2,4-dinitrophenyl)rhodamine B-hydrazide for detection of Cu(II) are disclosed. The preparing method includes a step of reacting rhodamine B acid chloride and 2,4-dinitrophenylhydrazine to generate the N-(2,4-dinitrophenyl)rhodamine B-hydrazide. The N-(2,4-dinitrophenyl)rhodamine B-hydrazide itself is free of color and fluorescence, but can generate coloring and fluorescence generating reactions selectively with Cu<2+>, so that a colorless substance is turned into a system with excellent optical properties, and detection sensitivity and selectivity for the Cu<2+> are largely enhanced. Accordingly, the N-(2,4-dinitrophenyl)rhodamine B-hydrazide is suitable for high-selectivity and high-sensitivity detection of the Cu<2+>. The detection can be performed by a light absorption spectrum method and a fluorescence spectrum method.

The invention discloses a macromolecular pH fluorescent probe for cotton transfer printing and dyeing. The structural formula of the macromolecular pH fluorescent probe is shown in the description. After 2-poistion Rhodamine B with carboxyl and hydrazine hydrate react to generate lactam, lactam reacts with acryloyl chloride, monomers with Rhodamine groups are obtained, and finally the macromolecular pH fluorescent probe with specific fluorescent responses to H+ is synthesized through copolymerization. The macromolecular pH fluorescent probe has very good water solubility, the fluorescent responses to the pH from alkaline to acidulous are very remarkable, meanwhile, the macromolecular pH fluorescent probe can achieve hot melting to be softened at the temperature of 110 DEG C, and can transfer and adsorb to the surfaces of cotton fibers, and therefore the reaction extent of the fabric surface can be tracked and monitored conveniently in the steaming process. Whether the reaction is finished or not can be known conveniently in real time by monitoring the pH value of the fabric surface through the pH fluorescent probe in the steaming process, and therefore the reaction endpoint is precisely controlled.

The invention belongs to the field of nucleic acid reagent preparation and food quality detection, and particularly relates to fluorescent nucleic acid silver and a preparation method and application thereof. The fluorescent nucleic acid silver is a fluorescent DNA nano-silver cluster and is marked as DNA-AgNCs. The size of the nano-silver cluster is 3 nm. The faint yellow is displayed in a citrate buffer solution, fluorescent nucleic acid silver is excited through red light of 600 nm, high fluorescence-emission occurs at the position of 666 nm, dipyridyl ruthenium is used for contrast, and the fluorescence quantum yield is 0.2. The fluorescent nucleic acid silver and the citrate buffer solution are mixed to obtain a nucleic acid silver solution, a BSA solution is added to the nucleic acid silver solution under the condition that the molar ratio of DNA to BSA is 1:1, a BSA-DNA-AgNCs stable fluorescence system can be obtained, and the fluorescent nucleic acid silver can be applied to qualitative and quantitative analysis of the content of ferrum in milk powder.

In a sensor system, an active sensor chip includes an array of periodically-patterned dielectric active sensor patches of different periodicities and geometries formed on a metal film. A specimen under study is positioned on each patch, and the active sensor chip is interrogated by illumination the patches in a predetermined sequence to result in a fluorescence response from each patch enhanced by SPP. The intensity of the fluorescence response is controlled by varying the wavelength, incidence angle, azimuthal orientation and polarization direction of the excitation light beam as the function of the periodicity of the illuminated patch. The system is compatible with commercial fluorescence microscopes and scanned laser interrogation systems.

The invention belongs to the technical field of gene engineering, and particularly relates to a hog cholera virus detection method and kit based on a G4-ThT biosensor and NASBA, and the specific method comprises the following steps: taking virus RNA extracted from a hog cholera virus sample as an amplification object, carrying out isothermal amplification by adopting an isothermal nucleic acid amplification technology, then adding a buffer solution containing Tris-HCl and KCl, and carrying out isothermal amplification by adopting an isothermal nucleic acid amplification technology; then thioflavin T is added to promote G-quadruplex on an amplification product to be folded and form a compound, fluorescence is detected under the excitation condition of a light source with a specific wavelength, and judgment is carried out according to the intensity of the fluorescence. The invention provides a detection method and a kit which do not need special biotin labeling or sulfydryl modification treatment probes, and the detection cost is obviously reduced.

The invention discloses an enhanced azo Salen Schiff base fluorescent probe and synthesis and application thereof, belonging to the technical field of fluorescent probes. The chemical formula of the fluorescent probe is C39H34N8O7, and the structural formula of the fluorescent probe is as shown in the specification. The fluorescent probe has good selectivity on Al <3+>, good linear relation, highsensitivity, strong fluorescence response, low detection limit and low cost, and can be widely applied to detection of Al<3+> ions in an environment. Compared with the prior art, the fluorescent probeof the invention has the advantages that more than one time of coordination sites can be provided; the fluorescent probe is more easily coordinated with Al<3+> to form a binuclear complex with a plurality of coordination sites; and molecular plane rigidity is enhanced after coordination, so the photoinduced electron transfer effect of Schiff base nitrogen atoms is inhibited, and a strong chelating fluorescence enhancement effect is generated. The synthesis method of the probe is simple, and has high yield, and reaction conditions are easy to control.

The invention relates to a use method for a creatinine content detection kit, which includes the following steps: adding creatinine of different concentrations to a buffer solution with the pH value of 7.3 and containing 100 mM of MHEPES, 150 mM of sodium chloride, 5 mM of potassium chloride and 1.3 mM of calcium chloride; obtaining and metering 150 muL sample solution through a capillary tube, inserting the tube in the kit and measuring through a capillary tube. The method provided by the invention has high selectivity; creatininase as proteinase has high specificity to a reactant, only catalyzes the hydrolysis reaction of creatinine, and has no catalytic activity to other oligomers; as three high selectivity enzymes are related during the detection, the selectivity in the method is increased exponentially, the final response of the kit is fluorescent response, and compared with other methods for detecting color change, the method adopting the fluorescent response has the advantage that the inherent characteristic of fluorescent response endows the method with higher sensitivity and anti-interference capability.

The invention relates to an organic framework material for evaluating the jaundice level of a human body as well as a preparation method and application thereof. The method is characterized in that arare earth post-functionalized metal-organic framework material composed of zirconium tetrachloride and 1, 2, 4, 5-benzenetetracarboxylic acid Is adopted, and the preparation method comprises the following steps: preparing UiO-66(COOH)2 from the zirconium tetrachloride and the 1, 2, 4, 5-benzenetetracarboxylic acid by virtue of a heating reflux method, dispersing the UiO-66(COOH)2 in an aqueous solution of europium nitrate, stirring at room temperature to prepare an activated metal organic framework material UiO-66(COOH)2: Eu, and dropwise dispensing the metal-organic framework material on porous test paper through ethanol dispersion to obtain fluorescent test paper which can be used for measurement of bilirubin in human serum. Compared with the prior art, the organic framework material has the advantages that the content of bilirubin in human serum can be rapidly and effectively obtained through human eyes or color reading software, accurate and rapid detection of the content of bilirubin in human serum is achieved, operation is rapid, and the organic framework material has a potential application value in the field of material science.

The invention discloses a bis-Schiff base aluminum ion fluorescent probe as well as a synthesis method and application thereof, and belongs to the technical field of fluorescent probes. The chemical formula of the bis-Schiff base aluminum ion fluorescent probe is C23H18N2O4F3Cl, and the structural formula of the bis-Schiff base aluminum ion fluorescent probe is shown in the specification. The bis-Schiff base aluminum ion fluorescent probe has strong fluorescence response, good selectivity, high sensitivity and low detection limit on Al < 3 + >, can be recognized by naked eyes and can be widelyapplied to detection of Al < 3 + >. The synthesis method of the bis-Schiff base aluminum ion fluorescent probe is simple, high in yield and suitable for industrial production. According to a method for preparing 3, 5-diamino-4-chlorobenzotrifluoride, the reaction yield is high, the conditions are easy to control, the operation is simple, an organic solvent can be recycled, and the pollution is small.

The invention discloses a method for detecting the content of sulfur in marine fuel oil by using tin dioxide quantum dots. The method comprises the following steps: carrying out hydrothermal treatment on a tin dioxide quantum dot solution at 180-200 DEG C for 5-6 hours; standing the stannic oxide quantum dot solution, taking out supernatant liquid, and adding absolute ethyl alcohol with the same volume to obtain a stannic oxide quantum dot ethanol solution; adding a fuel oil solution with the known sulfur content into the tin dioxide quantum dot ethanol solution, and measuring the fluorescence intensity of the tin dioxide quantum dots; adding the n-butyl sulfide solution for multiple times, calculating the sulfur content after each addition, respectively measuring the fluorescence intensity, and establishing a characteristic curve between the sulfur content and the fluorescence intensity; and adding the fuel oil to be detected into the tin dioxide quantum dots, measuring the fluorescence intensity, and substituting the fluorescence intensity into the characteristic curve to obtain the sulfur content. The content of sulfur in the fuel oil can be effectively detected by utilizing the tin dioxide quantum dots, and the method is simple and easy to operate, high in feasibility, good in chemical stability, low in cost and non-toxic.

The invention belongs to the technical field of small organic molecule fluorescent probes, particularly relates to a fluorescent probe for detecting hypochlorous acid, a preparation method and applications thereof, and provides a fluorescent probe, which has a structure represented by a formula (I). According to the present invention, the fluorescent probe has strong selectivity on hypochlorous acid, and can generate strong fluorescence response after being reacted with hypochlorous acid; when the fluorescence probe with a concentration of 5 [mu]M reacts with hypochlorous acid with a concentration of 0-150 [mu]M, the fluorescence response is enhanced along with the increase of the concentration of hypochlorous acid; and the fluorescence probe has advantages of high sensitivity, good selectivity and good response to hypochlorous acid.

The invention discloses an 8-aminoquinoline amide derivative, a preparation method, application and a fluorescence analysis method thereof. The preparation method of the 8-aminoquinoline amide derivative comprises the following steps that (1a) N-bromoacetyl-8-aminoquinoline, propargylamine and acetonitrile are weighed, the N-bromoacetyl-8-aminoquinoline, propargylamine and the acetonitrile are dissolved in the acetonitrile, and then potassium carbonate is added to obtain a reaction mixture; (2a) the reaction mixture obtained in the step (1a) is subjected to stirring reaction at room temperature, filtration is performed, a solvent is removed, and then the 8-aminoquinoline amide derivative is obtained through column chromatography elution. The 8-aminoquinoline amide derivative can be appliedto the field of fluorescent probes. Zn<2+> and Cd<2+> can be simultaneously detected through the derivative, the preparation method, application and the fluorescent analysis method performed based onthe derivative.

The invention discloses a kit for detecting bleomycin based on a double-ring hairpin probe and an enzyme-mediated cascade amplification strategy and a detection method of the kit. The double-ring hairpin probe can effectively silence a trigger sequence and inhibit non-specific hybridization, and therefore a background signal of the detection method is reduced; an enzyme-mediated cascade amplification reaction can achieve multiple amplification of a detection signal, therefore, the sensitivity of the detection method is improved, high-sensitivity detection of bleomycin is achieved, the linear range is 40 pM to 10 nM, and the detection limit is 35 pM. The sensitivity is improved by about two orders of magnitudes compared with a previously established label-free fluorescence detection method, and the requirements of clinical sample detection can be met.

The invention relates to a creatinine content detecting reagent and kit and manufacturing and using methods of the kit. The creatinine content detecting reagent comprises creatininase, creatinase, creatine oxidase and horseradish peroxidase; and a fluorescent dye, i.e., 5(6)-carboxyl-2,7-dichlordihydro fluorescein ester dipivalate comprises a dye bottom layer, a protease fixing layer and a top layer protecting layer. The using method has high selectivity; a protease has high specificity to reactants; the creatininase can be only used for catalyzing the hydrolysis reaction of creatinine, and does not have catalysis activity on other reaction substrates; three enzymes with high specificity are related to the detection process, so that the selectivity of the method is increased exponentially; the response fed by the kit is fluorescent response; and compared with certain methods for detecting color variations, the invention has the advantages of high sensitivity and interference resistance under the action of the inherent characteristics of fluorescent response.

The invention discloses a kit for detecting bleomycin based on a double-ring hairpin probe and an enzyme-mediated cascade amplification strategy and a detection method of the kit. The double-ring hairpin probe can effectively silence a trigger sequence and inhibit non-specific hybridization, and therefore a background signal of the detection method is reduced; an enzyme-mediated cascade amplification reaction can achieve multiple amplification of a detection signal, therefore, the sensitivity of the detection method is improved, high-sensitivity detection of bleomycin is achieved, the linear range is 40 pM to 10 nM, and the detection limit is 35 pM. The sensitivity is improved by about two orders of magnitudes compared with a previously established label-free fluorescence detection method, and the requirements of clinical sample detection can be met.

The invention relates to a fluorescent probe molecule for detecting explosive RDX and its preparation method and application. The method uses pyrrole and p-tolualdehyde as reactants to first obtain chlorofluoroboron dipyrrole derivatives, and then combine them with Hydrazine hydrate was reacted at room temperature for 12 hours, and then separated by column chromatography to obtain a fluorescent probe molecule substituted with a hydrazine group. Furthermore, RDX can be detected indirectly through the response of the fluorescent probe to the photolysis product of explosive RDX. The method has mild reaction conditions and simple test conditions, and the probe molecules have high sensitivity and good selectivity to explosives RDX, which is a feasible attempt in the application of fluorescent sensors to explosives detection.

The invention discloses an organic ligand for synthesizing metal organic frames. The chemical nomenclature of the organic ligand is 2-(3, 5-bis (4-pyridyl) phenyl) benzoglioxaline. The chemical formula of the organic ligand is represented in the description. The invention also discloses a synthetic method of the organic ligand and a Cu (I) ion-based bright yellow metal organic frame (MOF-1) and a Cu (I) ion-based dark red metal organic frame (MOF-2), and the chemical formulas are {[Cu(L)I].0.5CH3CN.0.5CH3OH.0.75H2O}n, (n=1,2,3...'8') and {[Cu(L)I].2H2O}n(n=1,2,3...'8'). The invention also provides a synthetic method for dark red Cu (I) ion metal organic frame (MOF-2) and a synthetic method for organic matters of the Cu (I) ion metal organic frame. The invention also provides application of the organic ligand and metal organic frames serving as alcohols small organic molecule fluorescence sensing materials. The application operation is simple, the cost is low, the respond is quick, and the obvious fluorescence response for methanol, ethanol, isopropanol , n-propanol under the liquid phase and the gas phase can be achieved.

The present invention relates to polymerisable fluorescent functional monomers in the structure as shown, and they have strong fluorescent reaction and part capable of reacting with biomacromolecular protein and non-covalent bond of DNA mutually. They may be polymerized with hydrophilic monomer to obtain fluorescent amphiphilic polymer with excellent biocompatibility, medicine carrying capacity and target administration capacity, and the polymer may find wide application foreground in high sensitivity fluorescent tracing detection technology, biomacromolecule separation and distinction, biosensor, medical diagnosis, medicine conveying, target material and other aspects. The present invention has reasonable synthesis path, simple synthesis process, easy-to-obtain material and high application value.

The invention discloses a rhodamine 6G hydrazide derivative, a preparation method of the derivative and an application of the derivative, and a method for carrying out fluorescence analysis on hypochlorous acid by using the derivative as a fluorescence probe. The rhodamine 6G hydrazide derivative is selectively reacted with hypochlorous acid to rapidly generate an obvious fluorescence enhancement effect. The rhodamine 6G hydrazide derivative disclosed by the invention is capable of realizing high-sensitivity and high-selectivity quantitative detection on trace hypochlorous acid in water and cells, and wide in application prospect in the fields of environment, biology and the like.

The invention discloses a rare earth organic framework material for fluorescence detection of trace water and a preparation method of the rare earth organic framework material. The rare earth organic framework material is an orderly microporous crystalline material, and the general structural formula of the material is [(CH3)2NH2]2[Ln6(mu3-OH)8(L-NH2)6(H2O)6]*(G)7, wherein Ln in the formula is Eu<3+>, Tb<3+> or Gd<3+>, L-NH2 is a chainlike phthalic acid organic ligand with an amino group function group and G represents a solvent in pores. The rare earth organic framework material is synthesized with a solvothermal method, the synthesis method adopts a simple process, the condition is mild, the yield is higher, and the product stability is good. The rare earth organic framework material has the properties of characteristic emission of rare earth and fluorescence of the ligand, the specific value of two emission peaks and the content of water in the solvent are in a better linear relation, and ratio type detection of trace water can be realized; the light color remarkably changes with the change of the water content, and the material can be used for colorimetric type detection of trace water. The sensitivity of a probe is high, the response speed is high, the detection limit is low, and the material can be recycled and is expected to be used practically in fields of detection and monitoring of trace water.

The invention discloses an 8-aminoquinoline amide derivative, a preparation method, an application and a method for fluorescence analysis thereof, wherein the preparation method of the 8-aminoquinoline amide derivative comprises the following steps: 1a) weighing N -Bromoacetyl-8-aminoquinoline, propynylamine and acetonitrile, then N-bromoacetyl-8-aminoquinoline and propynylamine are dissolved in acetonitrile, and then potassium carbonate is added to obtain a reaction mixture; 2a ) The reaction mixture obtained in step 1a) is stirred and reacted at room temperature, then filtered and removed from the solvent, and then eluted by column chromatography to obtain 8-aminoquinoline amide derivatives, the 8-aminoquinoline amide derivatives The substance can be applied to the field of fluorescent probes, and the derivative, preparation method, application, and method for fluorescence analysis based on the derivative can simultaneously detect Zn 2+ and Cd 2+ to test.

The invention discloses a fluorescent reagent and a preparation method for stepwise detection of trace amounts of trivalent aluminum ions and divalent lead ions, belonging to the field of fluorescent chemical sensors. The fluorescent reagent consists of PPPNa and a good solvent. 3,4-dibromo-1,2,5-three (methoxycarbonylphenyl) pyrrole is dissolved in good solvent, add 4-methoxycarbonylphenylboronic acid, catalyst and basic salt, react to obtain PPP; PPP Dissolve in a solvent, add alkali, and react to obtain PPPNa. The PPPNa is dissolved in a good solvent to obtain a fluorescent reagent; the fluorescent reagent can quantitatively detect aluminum ions or divalent lead ions in aqueous solution or mixed salt solution, the preparation method is simple, the operation is convenient, the response is fast, and it has high sensitivity It can completely break away from the dependence on high-precision instruments according to actual needs, and will largely meet people's detection requirements for aluminum ions and lead ions today.

The invention discloses N-(2,4-dinitrophenyl)-rhodamine B hydrazide and a preparation method and an application of the N-(2,4-dinitrophenyl)-rhodamine B hydrazide. The rhodamine B derivative provided by the invention is N-(2,4-dinitrophenyl)-rhodamine B hydrazide, the structural formula of which is I. The N-(2,4-dinitrophenyl)-rhodamine B hydrazide, is colorless and free from fluorescence, but can selectively react with Cu<2+> ions in chromogenic and fluorescent opening reaction. The colorless substance generates a system with excellent optical performance, so that the detection sensitivity and selectivity of Cu<2+> ions are greatly improved. Therefore, the N-(2,4-dinitrophenyl)-rhodamine B hydrazide is appropriate for high selectivity and high sensitivity detection for the Cu<2+> ions. Detection can be carried out by a light absorption spectrum and a fluorescence spectrum. (Formula I is shown in the specification).