Rhodamine 6G hydrazide derivative, preparation method of derivative and application of derivative, and method for carrying out fluorescence analysis on hypochlorous acid by using derivative as fluorescence probe

A technology for hydrazide derivatives and fluorescence analysis, which is applied in the fields of fluorescence/phosphorescence, biological testing, material excitation analysis, etc., can solve problems such as cumbersome procedures, restrictions on practical applications, and complex technologies, and achieve broad application prospects and stable fluorescence detection signals , the effect of high sensitivity

Inactive Publication Date: 2014-06-25
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the synthesis process of these reagents is cumbersome and technically complex, whi

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  • Rhodamine 6G hydrazide derivative, preparation method of derivative and application of derivative, and method for carrying out fluorescence analysis on hypochlorous acid by using derivative as fluorescence probe
  • Rhodamine 6G hydrazide derivative, preparation method of derivative and application of derivative, and method for carrying out fluorescence analysis on hypochlorous acid by using derivative as fluorescence probe
  • Rhodamine 6G hydrazide derivative, preparation method of derivative and application of derivative, and method for carrying out fluorescence analysis on hypochlorous acid by using derivative as fluorescence probe

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Example Embodiment

[0031] Example one

[0032] The preparation method of rhodamine 6G hydrazide derivative of the present invention includes the following steps:

[0033] 1) Weigh a certain amount of rhodamine 6G, ethanol and hydrazine monohydrate with a mass concentration of 80%, and dissolve rhodamine 6G in ethanol, and then add hydrazine monohydrate dropwise, then reflux and filter to obtain After precipitation, washing and drying, a pink solid rhodamine 6G hydrazide is obtained, wherein the ratio of rhodamine 6G, ethanol and hydrazine monohydrate is 1.0g:20mL:3mL;

[0034] 2) Weigh a certain amount of 1-naphthoyl chloride and acetonitrile, and then dissolve 1-naphthoyl chloride in acetonitrile to obtain a solution of 1-naphthoyl chloride in acetonitrile. The ratio of formyl chloride to acetonitrile is 100μL: 15mL;

[0035] 3) Weigh a certain amount of rhodamine 6G hydrazide and acetonitrile, and then dissolve rhodamine 6G hydrazide in acetonitrile to obtain the acetonitrile solution of rhodamine 6G...

Example Embodiment

[0040] Example two

[0041] The preparation method of rhodamine 6G hydrazide derivative of the present invention includes the following steps:

[0042] 1) Weigh a certain amount of rhodamine 6G, ethanol and hydrazine monohydrate with a mass concentration of 80%, and dissolve rhodamine 6G in ethanol, and then add hydrazine monohydrate dropwise, then reflux and filter to obtain After precipitation, washing and drying, a pink solid rhodamine 6G hydrazide is obtained, wherein the ratio of rhodamine 6G, ethanol and hydrazine monohydrate is 1.0g:30mL:2mL;

[0043] 2) Weigh a certain amount of 1-naphthoyl chloride and acetonitrile, and then dissolve 1-naphthoyl chloride in acetonitrile to obtain a solution of 1-naphthoyl chloride in acetonitrile. The ratio of formyl chloride to acetonitrile is 90μL: 13mL;

[0044] 3) Weigh a certain amount of rhodamine 6G hydrazide and acetonitrile, and then dissolve rhodamine 6G hydrazide in acetonitrile to obtain the acetonitrile solution of rhodamine 6G ...

Example Embodiment

[0049] Example three

[0050] The preparation method of rhodamine 6G hydrazide derivative of the present invention includes the following steps:

[0051] 1) Weigh a certain amount of rhodamine 6G, ethanol and hydrazine monohydrate with a mass concentration of 80%, and dissolve rhodamine 6G in ethanol, and then add hydrazine monohydrate dropwise, then reflux and filter to obtain After precipitation, washing and drying, a pink solid rhodamine 6G hydrazide is obtained, wherein the ratio of rhodamine 6G, ethanol and hydrazine monohydrate is 1.0g:25mL:4mL;

[0052] 2) Weigh a certain amount of 1-naphthoyl chloride and acetonitrile, and then dissolve 1-naphthoyl chloride in acetonitrile to obtain a solution of 1-naphthoyl chloride in acetonitrile. The ratio of formyl chloride to acetonitrile is 115μL:11mL;

[0053] 3) Weigh a certain amount of rhodamine 6G hydrazide and acetonitrile, and then dissolve rhodamine 6G hydrazide in acetonitrile to obtain the acetonitrile solution of rhodamine 6...

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Abstract

The invention discloses a rhodamine 6G hydrazide derivative, a preparation method of the derivative and an application of the derivative, and a method for carrying out fluorescence analysis on hypochlorous acid by using the derivative as a fluorescence probe. The rhodamine 6G hydrazide derivative is selectively reacted with hypochlorous acid to rapidly generate an obvious fluorescence enhancement effect. The rhodamine 6G hydrazide derivative disclosed by the invention is capable of realizing high-sensitivity and high-selectivity quantitative detection on trace hypochlorous acid in water and cells, and wide in application prospect in the fields of environment, biology and the like.

Description

technical field [0001] The present invention relates to a derivative for detecting hypochlorous acid, its preparation method, application and detection method, in particular to a rhodamine 6G hydrazide derivative, its preparation method, application and its use as a fluorescent probe to perform fluorescence on hypochlorous acid method of analysis. Background technique [0002] Hypochlorous acid (HOCl) is a strong oxidizing agent that can play an antimicrobial role in environmental water treatment processes and living organisms. In daily life, hypochlorous acid has been widely used as a tap water disinfectant because of its good bactericidal effect, low price and convenient use. However, high-concentration hypochlorous acid solution is highly irritating, harmful to the respiratory system, and easily reacts with organic matter in water to generate serious carcinogens such as chloroform and carbon tetrachloride, which are potentially harmful to human health. In living organis...

Claims

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Application Information

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IPC IPC(8): C07D491/107C09K11/06G01N21/64
CPCC07D491/107C09K11/06C09K2211/1088G01N21/64G01N33/52
Inventor 张祯邓成权孟列素颜晓梅
Owner XI AN JIAOTONG UNIV
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