The invention provides a preparation method of a Ru nano-material with different morphologies and application of the preparation method. The preparation method of the Ru nano-material with the different morphologies comprises the steps that (1) a Ru source is added into ethylene glycol for sufficient and uniform stirring; (2) then different gas is introduced, and reaction, cooling, washing and drying are carried out under 120-200 DEG C; and (3) an obtained catalyst and carbon black are mixed uniformly, drying is carried out after uniform ultrasound, then thermal treatment is carried out to obtain Ru/C, and the Ru/C is used for an electrochemical performance test. Ru is uniformly distributed on the carbon black, and Ru presents a shape of a nanowire, a nano sheet clip and nano-particles; the OER performance of the Ru nanowire, the nano sheet clip and the nano-particles greatly exceeds industrial RuO2 and industrial IrO2 catalysts under the acidic condition, and voltages corresponding tothe electric current density of 10mA/cm<2> of the OER performance of the Ru nanowire, the nano sheet clip and the nano-particles separately reach 224mV, 286mV and 252mV (vs.RHE) under the acidic condition. The catalyst has good stability under the acidic condition. The preparation method of the Ru nano-material with the different morphologies is simple, the catalyst has higher activity and stability, and a better application prospect is achieved on the aspect of electrolysed water.