Use method for creatinine content detection kit

Abstract

The invention relates to a use method for a creatinine content detection kit, which includes the following steps: adding creatinine of different concentrations to a buffer solution with the pH value of 7.3 and containing 100 mM of MHEPES, 150 mM of sodium chloride, 5 mM of potassium chloride and 1.3 mM of calcium chloride; obtaining and metering 150 muL sample solution through a capillary tube, inserting the tube in the kit and measuring through a capillary tube. The method provided by the invention has high selectivity; creatininase as proteinase has high specificity to a reactant, only catalyzes the hydrolysis reaction of creatinine, and has no catalytic activity to other oligomers; as three high selectivity enzymes are related during the detection, the selectivity in the method is increased exponentially, the final response of the kit is fluorescent response, and compared with other methods for detecting color change, the method adopting the fluorescent response has the advantage that the inherent characteristic of fluorescent response endows the method with higher sensitivity and anti-interference capability.

Description

[0001] This application is a creatinine content detection reagent and kit, and a divisional application for the production and use of the kit. The original application date was September 20, 2011, the original application number: 2011102799693, and the name of the invention: a Creatinine content detection reagent and kit, and methods for making and using the kit. technical field [0002] The invention belongs to the field of creatinine detection, in particular to a creatinine content detection reagent, a kit, and a method for making and using the kit. Background technique [0003] Creatinine is a product of creatine phosphate metabolism, and its chemical structure is a lactam formed by dehydration condensation of creatine. Creatinine is generally present in the blood, passes through the blood circulation to the kidneys and is filtered out. For people with normal kidney function, the concentration of creatinine in the blood varies from person to person. Generally speaking, ...

AI Technical Summary

Problems solved by technology

Mass spectrometry and liquid chromatography cannot be automated, the operation is complicated, and requires the analysis team to operate and maintain the instrument, while the ELISA method requires the use of an expensive microplate reader. Although it has high sensitivity, it has poor selectivity and often Overestimate the concentration of creatinine, leading to overdose or underdose of clinical medication

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