Organic framework material for evaluating jaundice level of human body as well as preparation method and application thereof

An organic framework and metal-organic framework technology, applied in the field of rare earth post-functionalized metal-organic framework materials and their preparation, can solve the problems of unclear biological toxicity, complex synthesis route, poor chemical stability, etc., and achieve a large change in emission peak intensity. , The preparation process is simple and the crystallinity is good.

Inactive Publication Date: 2020-11-27
EAST CHINA NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The fluorescent materials for detecting bilirubin in the prior art are mainly organic polymers and fluorescent molecularly modified proteins, but their large-scale production and use are limited due to their complex synthetic routes, poor chemical stability, and unclear biological toxicity.

Method used

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  • Organic framework material for evaluating jaundice level of human body as well as preparation method and application thereof
  • Organic framework material for evaluating jaundice level of human body as well as preparation method and application thereof
  • Organic framework material for evaluating jaundice level of human body as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1: UiO-66(COOH) 2 preparation of

[0025] 17mmol of 1,2,4,5-benzenetetracarboxylic acid and 10mmol of zirconium tetrachloride were dissolved in 50mL of deionized water, and the solution was ultrasonically treated for 20 minutes until clear, and then moved to a 100mL three-necked flask (the middle of the three-necked flask was Buckle and connect the flowing water condenser, and seal the other two ports with rubber stoppers). Put the three-necked flask in methyl silicone oil, and after 100°C oil bath treatment for 24 hours, the obtained product was centrifuged and washed with deionized water several times until the pH was neutral to remove unreacted 1,2,4,5-benzene Tetraformic acid continued to be dispersed in 50mL of deionized water, and moved to a 100mL three-necked flask (the middle of the three-necked flask was connected to a water condenser, and the other two ports were sealed with rubber stoppers). The three-necked flask was placed in methyl silicone oil, tre...

Embodiment 2

[0035] The metal organic framework material UiO-66 (COOH) that 30mg embodiment 1 prepares 2 : Eu was dispersed in 15mL of absolute ethanol, and ultrasonically treated for 20mins to make it completely dispersed. Then use a dropper to mix the dispersion evenly and drop-coat it on 2*4cm filter paper. After repeated drop-coating five times, put the filter paper in a ventilated place to dry in the shade, and you can get a fluorescent test paper for measuring the concentration of bilirubin in human serum. Under the excitation of ultraviolet light, the fluorescent test paper maintains the interaction with UiO-66(COOH) in solid and liquid phases. 2 : While Eu has the same optical properties, there is also a certain dependence on the bilirubin in human serum, that is, the fluorescence of the ligand 1,2,4,5-benzenetetracarboxylic acid and the obvious europium Ion characteristic fluorescence, and maintain the characteristics of fluorescence detection of bilirubin.

[0036] Human serum ...

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Abstract

The invention relates to an organic framework material for evaluating the jaundice level of a human body as well as a preparation method and application thereof. The method is characterized in that arare earth post-functionalized metal-organic framework material composed of zirconium tetrachloride and 1, 2, 4, 5-benzenetetracarboxylic acid Is adopted, and the preparation method comprises the following steps: preparing UiO-66(COOH)2 from the zirconium tetrachloride and the 1, 2, 4, 5-benzenetetracarboxylic acid by virtue of a heating reflux method, dispersing the UiO-66(COOH)2 in an aqueous solution of europium nitrate, stirring at room temperature to prepare an activated metal organic framework material UiO-66(COOH)2: Eu, and dropwise dispensing the metal-organic framework material on porous test paper through ethanol dispersion to obtain fluorescent test paper which can be used for measurement of bilirubin in human serum. Compared with the prior art, the organic framework material has the advantages that the content of bilirubin in human serum can be rapidly and effectively obtained through human eyes or color reading software, accurate and rapid detection of the content of bilirubin in human serum is achieved, operation is rapid, and the organic framework material has a potential application value in the field of material science.

Description

technical field [0001] The invention relates to the technical field of preparation of fluorescent recognition materials, in particular to a rare earth post-functionalized metal-organic framework material for evaluating human jaundice level and its preparation method and application. [0002] technical background [0003] Bilirubin is a kind of bile pigment, which is the main pigment in human bile and is orange-yellow. Bilirubin is the main metabolite of iron porphyrin compounds in the body. At the same time, bilirubin is an important basis for clinically judging jaundice and an important indicator of liver function. This is mainly because in the case of icteric hepatitis, the hepatitis virus destroys liver cells, destroys and remodels liver tissue, and blocks bile ducts, resulting in an increase in the level of bilirubin in serum. Specifically, it is clinically believed that the normal range of free bilirubin in human serum is between 0-17.7 μmol / L, and when the bilirubin c...

Claims

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Application Information

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IPC IPC(8): C08G83/00C09K11/06G01N21/64
CPCC08G83/008C09K11/06C09K2211/183G01N21/6402
Inventor 李会利夏超曹蒙蒙夏金峰蒋丹宇周国红
Owner EAST CHINA NORMAL UNIVERSITY
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