Fluorescent probe molecule for detecting explosive rdx and its preparation method and application
A technology of fluorescent probes and probe molecules, applied in the field of fluorescent probes, can solve problems such as difficult online detection, and achieve the effects of convenient post-processing, good fluorescence response, and simple synthesis route.
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Embodiment 1
[0040] The preparation of fluorescent probe molecules, the basic synthesis process is as follows:
[0041] (1) Synthesis of chlorofluoroboron dipyrrole derivatives: 0.5 mL of concentrated hydrochloric acid was dissolved in 100 mL of distilled water, and placed in a 250 mL flask. Add 57.8 mmol of pyrrole, stir until clear, and then add 9.63 mmol of p-tolualdehyde. After reacting at room temperature for 1 h, it was quenched with 2 mL of ammonia water and filtered. The obtained solid was washed three times with water, and then twice with petroleum ether to obtain a milky white crude product. Add 50mL of 9.63mmol NCS suspension in tetrahydrofuran dropwise within 1h, continue the reaction for 2h, add 50mL of water, extract with dichloromethane, and use anhydrous Na 2 SO 4 Drying yields compound 2. 2.4 mmol of compound 2 was dissolved in 25 mL of dichloromethane, and a dispersion system of 2.4 mmol of chloranil in 10 mL of dichloromethane was added dropwise to the reaction syste...
Embodiment 2
[0045] The preparation of fluorescent probe molecules, the basic synthesis process is as follows:
[0046] (1) Synthesis of chlorofluoroboron dipyrrole derivatives: 0.5 mL of concentrated hydrochloric acid was dissolved in 100 mL of distilled water, and placed in a 250 mL flask. Add 38.5 mmol of pyrrole, stir until clear, and then add 9.63 mmol of p-tolualdehyde. After reacting at room temperature for 1 h, it was quenched with 2 mL of ammonia water and filtered. The obtained solid was washed three times with water, and then twice with petroleum ether to obtain a milky white crude product. Add 50mL of 28.9mmol NCS suspension in tetrahydrofuran dropwise within 1h, continue the reaction for 2h, add 50mL of water, extract with dichloromethane, and use anhydrous Na 2 SO 4Drying yields compound 2. 2.4 mmol of compound 2 was dissolved in 25 mL of dichloromethane, and a dispersion system of 4.8 mmol of chloranil in 10 mL of dichloromethane was added dropwise to the reaction system...
Embodiment 3
[0049] Fluorescent probe molecules for the detection of explosives RDX:
[0050] Take 1000 μg / mL RDX standard solution and dilute it with acetonitrile-water to 5×10 -6 M, 1×10 -5 M, 2×10 -5 M,4×10 -5 M,6×10 -5 M,8×10 -5 M, 1×10 -4 M, 2×10 -4 M,4×10 -4 M, 6×10 -4 M, 8×10 -4 M, 1×10 -3 M; Take the above 10 μL RDX standard solution of different concentrations in a 2mL centrifuge tube, irradiate it under a 254nm portable ultraviolet lamp for 10min to decompose it; RDX solutions of different concentrations were mixed and placed for 10 minutes, then the above solution was transferred into a 1×1 cm quartz cuvette, placed in a fluorescence spectrometer for fluorescence spectrum scanning, and the excitation wavelength λ ex =365nm, emission wavelength λ em =542nm; the fluorescence spectrum after the reaction between the fluorescent probe molecule and the RDX photolysis product is shown in Figure 5 Shown; utilize fluorescence spectrometer to record the fluorescence intensit...
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