Fluorescent reagent for detecting specificity of aluminium ions based on aggregation-induced emission principle

A technology for fluorescent reagents and aluminum ions, which is applied in the field of fluorescent reagents for the specific detection of aluminum ions based on the principle of aggregation-induced luminescence, can solve the problems that have not been popularized in practical applications, the synthesis of detection reagents is complicated, and the sensitivity is low, and the cost of raw materials is achieved. Inexpensive, good specific fluorescence recognition, less environmental and human pollution effects

Inactive Publication Date: 2012-01-11
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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Problems solved by technology

Due to the shortcomings of these detection methods such as low sensitivity, poor selectivity, and complex synthesis of detection reagents, they are still in the stage of experimentation and development, and have not been popularized in practical applications.
The commonly used ion detection methods today often rely on expensive instruments such as atomic absorption spectroscopy and X-ray fluorescence spectroscopy, which are too costly and time-consuming

Method used

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  • Fluorescent reagent for detecting specificity of aluminium ions based on aggregation-induced emission principle
  • Fluorescent reagent for detecting specificity of aluminium ions based on aggregation-induced emission principle
  • Fluorescent reagent for detecting specificity of aluminium ions based on aggregation-induced emission principle

Examples

Experimental program
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Effect test

example 1

[0019] Example 1: Selective recognition test

[0020] Take a 100ml volumetric flask, prepare 100μM TriPP-COONa solution according to the volume ratio of tetrahydrofuran and water 1:3, take 4ml solution, inject 50μM different ions respectively. The 14 kinds of ions selected in this experiment are as follows (in order to eliminate the interference of negative ions, the tested samples all use metal nitrate solution): Al 3+ 、K + , Mg 2+ , Ca 2+ 、Ce 2+ , Hg 2+ , Fe 3+ 、Ag + 、Cu 2+ 、Cr 2+ 、Ba 2+ 、Ni 2+ , Pb 2+ , Zn 2+ . In order to compare the intensity of the fluorescence detection signal of different ions conveniently, we draw the maximum emission intensity of the fluorescent reagent containing different ions into a histogram (excitation wavelength 326nm), see figure 1 .

example 2

[0021] Example 2: Detection time test

[0022] Take 4ml of the TriPP-COONa fluorescence reagent provided in Example 1, first measure the fluorescence intensity of the blank fluorescence reagent before adding aluminum ions, then inject 50 μM aluminum ions into it, measure the fluorescence intensity immediately, and then measure the fluorescence every 30 seconds Intensity until the fluorescence intensity of the fluorescent reagent no longer changes significantly. The trend graph of fluorescence measured in this example over time is shown in figure 2 .

example 3

[0023] Example 3: Quantitative detection test

[0024] Get the TriPP-COONa fluorescent reagent provided by example 1, gradually increase the concentration of aluminum ions (from 1 μ M to 28 μ M), and the fluorescence spectrum of a series of fluorescent reagents obtained by exciting at 326nm is shown in image 3 (a), the small picture embedded in the upper left corner is the trend chart of the fluorescence intensity change at the maximum emission wavelength of 460nm, and the linear relationship chart in the concentration range from 1 μM to 11 μM is shown in image 3 (b).

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Abstract

The invention discloses a fluorescent reagent for detecting specificity of aluminium ions based on an aggregation-induced emission principle. The fluorescent reagent is represented as sodium4-(2, 5-diphenyl-1H-pyrrole-1-yl)benzoate (TriPP-COONa). The fluorescent reagent has fluorescence enhancement specific response to the aluminium ions, has the detection limit of 1 mu M (27 mu g/ L) which is lower than the aluminium ion concentration (no higher than 200 mu g/ L) in drinking water stipulated by the World Health Organization, is free of the interference of other ions, such as K+, Mg2+, Ca2+, Ba2+, Ni2+, and has short response time. The fluorescence intensity of the fluorescent reagent has good linear relationship within the aluminium ion concentration range of 1 mu M to 11 mu M. According to the linear equation in the invention, the quantitive detection of the concentration of aluminium ions in the water sample can be realized conveniently and rapidly.

Description

technical field [0001] The invention is based on the principle of "aggregation-induced luminescence", and relates to a fluorescent reagent with high selectivity, high sensitivity, and rapid quantitative detection of aluminum ions and a detection method thereof. Background technique [0002] The content of aluminum in the earth's crust is second only to oxygen and silicon, ranking third. It is the most abundant metal element in the earth's crust. Its application is extremely extensive and is closely related to people's lives. If you often drink water purified with aluminum salts, eat foods containing aluminum salts, such as deep-fried dough sticks (about 0.33 grams of alum in 100 grams of deep-fried dough sticks), jelly, canned beverages, etc., or often use aluminum cookers, it is easy to Make the aluminum element accumulate slowly in the human body. The toxicity caused by aluminum ions is slow and difficult to detect. When accumulated to a certain extent, neurotoxicity wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06
Inventor 董宇平韩天宇石建兵佟斌冯霄支俊格
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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