Creatinine content detecting reagent and kit, and manufacturing and using methods of kit

Abstract

The invention relates to a creatinine content detecting reagent and kit and manufacturing and using methods of the kit. The creatinine content detecting reagent comprises creatininase, creatinase, creatine oxidase and horseradish peroxidase; and a fluorescent dye, i.e., 5(6)-carboxyl-2,7-dichlordihydro fluorescein ester dipivalate comprises a dye bottom layer, a protease fixing layer and a top layer protecting layer. The using method has high selectivity; a protease has high specificity to reactants; the creatininase can be only used for catalyzing the hydrolysis reaction of creatinine, and does not have catalysis activity on other reaction substrates; three enzymes with high specificity are related to the detection process, so that the selectivity of the method is increased exponentially; the response fed by the kit is fluorescent response; and compared with certain methods for detecting color variations, the invention has the advantages of high sensitivity and interference resistance under the action of the inherent characteristics of fluorescent response.

Description

technical field [0001] The invention belongs to the field of creatinine detection, in particular to a creatinine content detection reagent, a kit, and a method for making and using the kit. Background technique [0002] Creatinine is a product of creatine phosphate metabolism, and its chemical structure is a lactam formed by dehydration condensation of creatine. Creatinine is generally present in the blood, passes through the blood circulation to the kidneys and is filtered out. For people with normal kidney function, the concentration of creatinine in the blood varies from person to person. Generally speaking, in the case of no heavy physical burden, the blood creatinine concentration of women is 45-60 μM, and the blood creatinine concentration of men fluctuates within the range of 60-110 μM. When there is a problem with renal function, the ability of the kidney to filter and remove creatinine will decrease, resulting in an abnormal increase in the concentration of creati...

AI Technical Summary

Problems solved by technology

Mass spectrometry and liquid chromatography cannot be automated, the operation is complicated, and requires the analysis team to operate and maintain the instrument, while the ELISA method requires the use of an expensive microplate reader. Although it has high sensitivity, it has poor selectivity and often Overestimate the concentration of creatinine, leading to overdose or underdose of clinical medication

Images