NASBA (Nucleic Acid Sequence Based Amplification) detection primer, probe and detection method for GII type norovirus in sea foods

A technology for virus detection and food, applied in the technical field of rapid detection of GII type norovirus in marine food, achieving high specificity and sensitivity, simple operation steps, and short detection time
CN103525947APending Publication Date: 2014-01-22YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2014-01-22

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Abstract

The invention discloses an NASBA (Nucleic Acid Sequence Based Amplification) detection primer, a probe and a detection method for GII type norovirus in sea foods. According to the NASBA detection primer, a forward primer sequence is represented as SEQ ID NO.1 and a backward primer sequence is represented as SEQ ID NO.2; the primer sequence of a molecular beacon probe for detecting the GII type norovirus is represented as SEQ ID NO.3. The detection method comprises the following steps: (1) constructing a standard cRNA (complementary Ribonucleic Acid) of the GII type norovirus; (2) carrying out a real-time NASBA reaction; (3) qualitatively and quantitatively analyzing. The primer and the probe disclosed by the invention have high specificity on the detection of the GII type norovirus. The detection method is simple in operation steps and short in detection time, can be used for carrying out a qualitative and quantitative analysis and has high specificity and sensitivity.
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Description

technical field

[0001] The invention relates to a technology for rapid detection of GII type norovirus in marine food based on real-time nucleic acid sequence-based amplification (Nucleic acid sequence-based amplification, NASBA) technology. Background technique

[0002] Norovirus (NVs) is a virus that exists in marine food and can cause acute gastroenteritis in adults and children. The norovirus genome is a single-stranded positive-sense RNA with a full length of 7642 nt, including three open reading frames (open reading frames, ORFs) ORFs1, ORFs2 and ORFs3. ORFs1 encodes a conserved non-structural protein with RNA polymerase activity; ORFs2 encodes a capsid protein with a molecular weight of about 56Kda; ORFs3 encodes a strongly basic microstructural protein with a molecular weight of about 22.5Kda, and some regions interact with the capsid protein , together forming the capsid. People are infected with norovirus mainly through contact with contaminated food and water so...

Claims

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