Primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through RPA-lateral flow assay technology

A technology of foot-and-mouth disease virus and lateral flow chromatography, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of no primer and probe design software, etc., and achieve high-sensitivity results

Active Publication Date: 2015-08-26
DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

RPA technology is in the initial research stage, there is no special primer and probe design so

Method used

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  • Primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through RPA-lateral flow assay technology
  • Primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through RPA-lateral flow assay technology
  • Primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through RPA-lateral flow assay technology

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Embodiment Construction

[0064] The present invention will be further described below in conjunction with the examples. It should be noted that the following descriptions are only for explaining the present invention and not limiting its content.

[0065] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0066]TwistAmp nfo Kits were purchased from TwistDX Company, Genline Hybriddetect-1lateral flow strips were purchased from Milenia GmbH (Germany), reverse transcription kit (Code No.: D2639A) was purchased from Treasure Bioengineering (Dalian) Co., Ltd., virus was extracted by magnetic bead method The DNA / RNA kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; the general detection primers and probes were synthesized by Shanghai Sangon Biotechnology Co., Ltd.

[0067] The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition, for examp...

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Abstract

The invention discloses a primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through a RPA-lateral flow assay technology. A primer probe liquid, popular primer and probes are arranged in the kit, wherein the primer probe liquid consists of a popular subtype primer which is used for detecting the foot-and-mouth disease virus through an RPA technology, and the probes, and the popular primer and the probes are in the primer probe liquid. The primer, the probe and the kit, which are disclosed by the invention, adopt the RPA-Lateral flow assay technology to establish a method for rapidly detecting the FMDV of cattle for the first time, and through the evaluation of specificity and evaluation of sensitivity, the sensitive, reliable and new method can be used for a clinical detection on site of the FMDV. Through the combination of the primer and the probes, which are disclosed by the invention, the method for detecting the FMDV in the aerosol in a subtype manner through the RPA technology has the advantages of being higher in sensitivity, specificity and repeatability.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a general-purpose and aerosol-based method for typing and detecting foot-and-mouth disease virus in aerosols by applying Recombinase Polymerase Amplification (RPA) technology combined with Lateral Flow Assay (Lateral Flow Assay) technology. Typing primers, probes and their kits. Background technique [0002] Foot-and-mouth disease is an acute, febrile, and highly contagious infectious disease caused by foot-and-mouth disease virus (FMDV) that occurs on cloven-hoofed animals such as pigs, cattle, and sheep. It is listed as "Class A severe infectious disease" by the World Organization for Animal Health. In case of an outbreak, the infected animals must be culled and contacted with the infected animals, causing huge direct economic losses, and seriously endangering the healthy development of animal husbandry and the foreign trade of related products, which has a profound imp...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/6844C12Q1/70C12Q2521/507C12Q2522/101C12Q2537/1376
Inventor 王洪梅何洪彬刘文浩贺文琦
Owner DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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