Method for amplifying nucleic acid sequence

A technology of nucleotide sequence and nucleic acid, applied in the field of synthetic DNA
CN100432237CInactive Publication Date: 2008-11-12TAKARA HOLDINGS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TAKARA HOLDINGS
Publication Date
2008-11-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
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Description

[0001] This application is a divisional application of an invention patent application with an application date of March 14, 2000, an application number of 00807534.4, and an invention title of "Method for Amplifying Nucleic Acid Sequence". technical field

[0002] The present invention relates to a method for synthesizing DNA, which can be used in the field of genetic engineering. It relates to a method of amplifying a nucleotide sequence used as a template and a method of detecting nucleic acids amplified by said method. Background technique

[0003] DNA synthesis is used for various purposes in research in the field of genetic engineering. With the exception of the synthesis of short strands of DNA such as oligonucleotides, most DNA synthesis is accomplished by enzymatic methods in which DNA polymerases are used. An example of an enzymatic method is the polymerase chain reaction (PCR) method, which is described in detail in US Pat. Nos. 4,683,195, 4,683,202, and 4,800,15...

Claims

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