Detection method and kit for transgenic soybean A2704-12 transformation event

Abstract

The invention discloses a method and a kit for rapidly detecting a transgenic soybean A2704-12 transformation event. The method comprises the following steps of: performing high-temperature denaturation on a sample DNA (deoxyribonucleic acid), and performing transcription by using a primer for identifying an A2704-12 specificity region under the action of RNA (ribonucleic acid) polymerase to produce RNA; taking the obtained RNA as a template, and performing NASBA (nucleic acid sequence based amplification) by using the primer; and finally detecting the amplified product. The method does not need to purchase a large instrument and thus reduces the detection cost; and the detected product is easy to degrade, thus reducing the problem of cross contamination among samples.

Description

technical field [0001] The invention relates to the field of transgenic plant detection, in particular to a method and a kit for detecting whether there is a transgenic soybean A2704-12 transformation event in a biological sample. Background technique [0002] The successful introduction of commercially interesting traits into plants by genetic manipulation in agriculture or industry is a complex process dependent on different factors. According to statistics from the International Agricultural Bioengineering Applied Technology Acquisition Authority (ISAAA), since the commercialization of genetically modified products, the planting area of ​​genetically modified crops has exceeded 100 million hectares. China has become the sixth largest growth country of GM crops. With the management of genetically modified products in various countries and people's attention to the safety of genetically modified products, countries around the world have issued relevant laws and policies to...

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Problems solved by technology

The above detection methods need to rely on expensive instruments, and because the amplification product is DNA, in order to prevent the mutual contamination of the experimental results caused by DNA, when designing the laboratory site, it is often necessary to combine sample extraction, sample amplification and sample detection. Distributed to different rooms and requires close and careful handling, which increases the complexity of the experiment

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